FACTORS RPF2 & RRS1 RECRUIT 5S RRNA & RPL5 & RPL11 INTO NASCENT RIBS

因素 RPF2

• 批准号: 7722264
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.11万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722264
• 关键词: Binding; Cell Nucleolus; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; DNA Sequence Rearrangement; Eukaryota; Eukaryotic Cell; Funding; Grant; Institution; Neighborhoods; Nucleoplasm; Process; Proteins; RNA, Ribosomal, 5S; Recruitment Activity; Research; Research Personnel; Resources; Ribonucleoproteins; Ribosomal Proteins; Ribosomal RNA; Ribosomes; Source; United States National Institutes of Health; Yeasts; cofactor; in vitro Assay; particle; rRNA Precursor; rib bone structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. More than 170 proteins are necessary for assembly of ribosomes in eukaryotes. However, cofactors that function with each of these proteins, substrates on which they act, and the precise functions of assembly factors--e.g., recruiting other molecules into preribosomes or triggering structural rearrangements of pre-rRNPs--remain mostly unknown. Here we investigated the recruitment of two ribosomal proteins and 5S ribosomal RNA (rRNA) into nascent ribosomes. We identified a ribonucleoprotein neighborhood in preribosomes that contains two yeast ribosome assembly factors, Rpf2 and Rrs1, two ribosomal proteins, rpL5 and rpL11, and 5S rRNA. Interactions between each of these four proteins have been confirmed by binding assays in vitro. These molecules assemble into 90S preribosomal particles containing 35S rRNA precursor (pre-rRNA). Rpf2 and Rrs1 are required for recruiting rpL5, rpL11, and 5S rRNA into preribosomes. In the absence of association of these molecules with pre-rRNPs, processing of 27SB pre-rRNA is blocked. Consequently, the abortive 66S pre-rRNPs are prematurely released from the nucleolus to the nucleoplasm, and cannot be exported to the cytoplasm.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在真核生物中核糖体的组装需要170多种蛋白质。但是，与这些蛋白质中的每种蛋白质，其作用的底物以及装配因子的确切功能（例如），将其他分子募集到质子体中或触发前RRNPS-雷神 - 雷神 - 雷神 - 主要是未知的结构重排的辅助因素。在这里，我们研究了两种核糖体蛋白和5S核糖体RNA（RRNA）中的新生核糖体。我们确定了含有两个酵母核糖体组装因子RPF2和RRS1，两个核糖体蛋白，RPL5和RPL11，以及5S rRNA的核糖体蛋白邻域，其中包含两个酵母核糖体组装因子。这四种蛋白质中每一种之间的相互作用已通过体外结合测定得到证实。这些分子组装成90S的含有35S rRNA前体（前RRNA）的前颗粒。 RPF2和RRS1是将RPL5，RPL11和5S rRNA募集到质子体中所必需的。在没有这些分子与前RRNP的关联的情况下，处理27SB前RRNA的处理被阻断。因此，流产的66S前RRNP过早地从核仁释放到核质，不能导出到细胞质。