A global evolutionary analysis of eukaryotic duplication processes

真核复制过程的全球进化分析

基本信息

批准号：
7408972
负责人：
Xiang Gao
金额：
$ 5.67万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-01-01 至 2009-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7408972
关键词：
Affect Age Age Distribution Animal Model Architecture Area Attention Bioinformatics Birth Birth Rate Blast Cell Caenorhabditis elegans Chromatin Structure Classification Code DNA Insertion Elements DNA Sequence Rearrangement DNA Transposable Elements Death Rate Demography Disease Drosophila melanogaster Eukaryota Eukaryotic Cell Event Evolution Exons Functional RNA Fungi Model Gene Duplication Gene Structure Genes Genetic Genome Genomics Goals Growth Hereditary Disease Human Indium Intercistronic Region Intergenic DNA Introns Invertebrates Investigation Lead Link Mammals Messenger RNA Methods Minor Molecular Evolution Mouse-ear Cress Mus Nucleic Acid Regulatory Sequences Open Reading Frames Organism Oryza sativa Plants Population Genetics Positioning Attribute Process Proteins Publishing RNA Splicing Rate Regulator Genes Relative (related person)Saccharomyces cerevisiae Site Source Standards of Weights and Measures Structure Surveys Techniques Variant Work base duplicate genes genome sequencing human disease insight novel paralogous gene physical process sample fixation size transposon/insertion element

项目摘要

DESCRIPTION (provided by applicant): Gene duplication is considered as the primary source of creating novel materials for genome evolution. It is also associated with many diseases of human genetic disorders. However, previous studies on gene duplication have been biased toward the events that involve the duplication of entire protein-coding genes (e.g., divergence of paralogs). Since duplication break-points arise completely independently of gene boundaries, many small duplication segments may happen within genes, i.e., internal gene fragment duplication. In this proposal, we plan to carry out genome-wide analyses to decipher whether/how gene architectures (e.g., intron birth) are affected duplication events that took place within genes during the evolution process (specific aim 1). In addition, no published studies have investigated the evolutionary process and effects on intergenic DNA regions through population genetics approach. Therefore, we propose to extend our analyses to intergenic DNAs to study their duplication dynamics and fixation process (specific aim 2). Through the proposed global analysis on the duplication processes in internal gene and intergenic region, both of which are poorly understood before, we will provide an unprecedented and unbiased spectrum of genome duplications in eukaryotes. We will carry out the global evolutionary analysis on duplication processes in eukaryotic model organisms with available whole genome sequences. For each organism, we will study the evolutionary demography of internal gene duplications, effects of internal duplication on gene structure variations, and the determinants of the fixation process on duplication products. Through characterization of the duplicated intergenic regions, we propose to identify the sequence with low divergence rate compare to their linked duplication regions as the potential functional elements in intergenic regions. Bioinformatics techniques, population genetics approaches and molecular evolution methods will be applied to achieve above goals. The results will be critical to understand a broad array of unsolved issues in evolutionary genetics, including the extent to which: duplication causes genomic expansion vs. contraction, internal duplication creates immediate changes in structure and function of duplicated genes, and the relative conserved non-coding region under selection, in addition to understanding the mutational basis of genetic disorders caused by duplication,