National Resource For Advanced NMR Technology

国家先进核磁共振技术资源

• 批准号: 9280017
• 负责人: William W Brey
• 金额: $ 166.39万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9280017
• 关键词: 2,4-Dinitrophenol; Address; Alzheimer' s Disease; Applied Research; Automobile Driving; Bacterial Drug Resistance; Basic Science; Biological; Biology; Biomedical Research; Biomedical Technology; Chemistry; Communities; Complement; Complex; Complex Mixtures; Crystallography; Diabetes Mellitus; Disease; Drug Targeting; Educational workshop; Electrons; Funding; Generations; Goals; HIV; High temperature of physical object; Hybrids; Insulin Resistance; International; Kinetics; Lead; Liquid substance; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Metabolic; Microbial Biofilms; Modeling; NMR Spectroscopy; Nuclear; Nuclear Magnetic Resonance; Proteins; Protons; Publications; RF coil; Research; Resolution; Resources; Roentgen Rays; Science; Series; Services; Signal Transduction; Structure; Techniques; Technology; Temperature; Time; Training; Training Activity; Tuberculosis; United States National Institutes of Health; Virus Replication; X-Ray Crystallography; design and construction; drug development; frontier; insight; instrument; instrumentation; interest; lectures; macromolecule; meetings; metabolomics; new technology; news; novel; open data; programs; protein complex; scientific organization; solid state nuclear magnetic resonance; structural biology; tool; web site

National Resource for Advanced NMR Technology OVERALL - Project Summary/Abstract Nuclear magnetic resonance (NMR) spectroscopy is a unique set of experimental tools for understanding the intricacies of biology from macromolecular complexes to complex mixtures, from atomic resolution structure to dynamics on timescales of picoseconds to kiloseconds, from chemistry to functional mechanisms and kinetic rates. No other technology has such breadth and potential for basic and applied research and for interfacing with other technologies, such as X-ray crystallography, small angle X-ray scattering, Cryo-EM, and many other spectroscopic tools. Structural characterization serves as the starting point, providing a framework for understanding biological activities, functional mechanisms and kinetic models that can be added by NMR. Dynamics can be exceptionally well characterized by NMR and this can lead to detailed understanding about how proteins and other macromolecules function, how complexes are formed, and sometimes how kinetic rates are achieved. The solution NMR spectroscopy of complex mixtures has been shown to be particularly useful in combination with mass spectrometry for metabolomics and other complex mixtures. Here, we focus on the frontiers of NMR technology made possible by recent breakthroughs in materials research and instrumentation, and their implementation for a broad user community interested in pursuing fundamental questions at atomic resolution at the forefront of biomedical research. Three TR&Ds advance the sensitivity of NMR each with novel technology – the first through use of high temperature superconductors for RF coils leading to unique sensitivity for solution NMR spectroscopy. TR&D2 takes advantage of a 600 MHz DNP instrument recently installed at the Magnet Lab that will provide enhanced sensitivity through the transfer of magnetization from electrons to protons. New and much more robust DNP probes with an expanded temperature range will be developed. TR&D3 takes advantage of the 36T Series Connected Hybrid for NMR spectroscopy – a jump in field strength of more that 50% equivalent to a jump in field strength from 17T (1990) to 23.5T (2016) that occurred over the past 26 years! This will lead to dramatic enhancements in sensitivity and even more spectacular reductions in signal averaging time. The science will be driven by an excellent team of DBPs and even more C&Ss that span a very broad range of science. A major team effort will be placed on training a new generation of NMR users through an annual pair of workshops and dissemination through publications and presentations at meetings, through a wide variety of scientific organizations and the news media; through a dedicated website for this Resource and through our training activities; as well as posting of our training lectures and video of demonstrations.

国家先进核磁共振技术资源 总体 - 项目摘要/摘要 核磁共振 (NMR) 波谱是一套独特的实验工具，用于了解 从大分子复合物到复杂混合物，从原子分辨率，生物学的复杂性 从皮秒到千秒时间尺度上的结构到动力学，从化学到功能机制 没有其他技术具有如此广泛的基础和应用研究潜力。 与其他技术连接，例如 X 射线晶体学、小角度 X 射线散射、Cryo-EM 和 许多其他光谱工具作为起点，提供了一个框架。 用于了解可通过 NMR 添加的生物活性、功能机制和动力学模型。 NMR 可以很好地表征动力学，这可以帮助我们详细了解 蛋白质和其他大分子如何发挥作用，复合物如何形成，有时还包括动力学如何 复杂混合物的溶液核磁共振光谱已被证明是特别有效的。 与代谢组学和其他复杂混合物的质谱结合使用非常有用。 材料研究和领域的最新突破使核磁共振技术的前沿成为可能 仪器，以及它们对有兴趣追求基本原理的广泛用户社区的实施 生物医学研究前沿的原子分辨率问题。 三个 TR&D 均采用新技术提高了 NMR 的灵敏度——第一个通过使用高 RF 线圈的温度超导体为溶液 NMR 光谱带来独特的灵敏度。 利用 Magnet 实验室最近安装的 600 MHz DNP 仪器，该仪器将提供增强的 通过将磁化强度从电子转移到质子来提高灵敏度 新的、更强大的 DNP。 TR&D3 将利用 36T 系列的优势开发温度范围更广的探头。 核磁共振波谱的互联混合 – 场强跃升超过 50%，相当于场强跃升 过去26年发生的场强从17T（1990年）到23.5T（2016年）这将导致戏剧性的变化！ 科学将提高灵敏度，甚至更显着地缩短信号平均时间。 由优秀的 DBP 团队以及涵盖非常广泛的科学领域的 C&S 推动。 团队的主要工作将集中在通过每年一对培训新一代 NMR 用户 讲习班并通过出版物和会议上的演讲、通过各种形式进行传播 科学组织和新闻媒体；通过该资源的专门网站以及我们的 培训活动；以及发布我们的培训讲座和演示视频。