Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

使用新型高温 Superco 在 24 T 场进行溶液相生物分子 NMR

基本信息

批准号：
8752012
负责人：
William W Brey
金额：
$ 17万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8752012
关键词：
Awareness Biomedical Research Biomolecular Nuclear Magnetic Resonance Calculi Cells Communities Community Healthcare Complex Crystallization Data Dependence Development Drug Industry Environmental Wind Face Feedback Filament Future Goals Heating High temperature of physical object Joints Lead Length Location Magnetic Resonance Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Membrane Proteins Nucleic Acids Operating System Pharmacologic Substance Phase Physiological Proteins Radiology Specialty Recording of previous events Research Research Personnel Resolution Resources Rest Role Running Sampling Science Signal Transduction Societies Solutions Spottings Structure System Techniques Technology Temperature Time United States National Institutes of Health base clinical Diagnosis cold temperature density design drug development improved insight instrument magnetic field next generation prevent prototype public health relevance research study screening tool wound

项目摘要

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 ¿L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.

描述（由申请人提供）：NMR 是学术界和工业生物医学界的核心。自 20 世纪 70 年代以来，NMR/MRI 磁体中使用的低温超导 (LTS) 线的最终限制阻碍了 NMR 的进一步发展。该提案建立在最近基于 NHMFL 的开发之上，该开发已交付第一个在稳定超导状态下运行的线圈。在 e34 T 领域，并提议利用这一突破来构建运行在 1 GHz 以上的全超导溶液核磁共振系统，以实现这一目标，并证明利用这些想法在该频率和更高频率下进行生物分子溶液态核磁共振的可行性。 (H30T) 磁场，我们的目标是：(i) 开发一种高均匀性圆形多丝 Bi-2212 线圈，一种新型高温超导体(HTS) 由 Larbalestier 等人于 2012 年开发，与之前提出的 HTS 不同，HTS 可以承载超过 30 T 的高电流密度；(ii) 在以持续模式运行的现有牛津仪器 LTS 外插入磁体中操作该线圈，以提供房间- 10 mm DSV 上的温度磁场最佳点超过 23.5 T，均匀性和相关场不稳定性为 H1- 2 ppm；(iii) 利用过去的专业知识十年来，NHMFL 在场稳定和探头构造方面积累了丰富的经验，以证明在室温下使用这种装置的可行性，其 H30 ppb 稳定性和均匀性超过 150 ¿ (iv) 作为 NHMFL 设施角色的一部分，将此系统置于整个 NMR 界的配置中，以便将其用于顺磁性、膜蛋白、细胞和蛋白质生物 NMR 研究，并获得有关方法的关键反馈进一步追求这一突破。