Radio Frequency Probe Development

射频探头开发

• 批准号: 7575463
• 负责人: William W Brey
• 金额: $ 14.45万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-20 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7575463
• 关键词: Affect; Area; Binding Proteins; Biological; Biological Assay; Cells; Chin; Detection; Development; Dialysis procedure; Frequencies; Health; Heating; Indium; Injection of therapeutic agent; Liquid substance; Medicine; Membrane; Membrane Proteins; Methods; Molecular Conformation; Mycobacterium tuberculosis; Performance; Pharmacologic Substance; Preparation; Proteins; Radio; Resolution; Sampling; Solutions; System; Techniques; Technology; Temperature; Testing; Tube; cell fixing; design and construction; electric field; improved; magnetic field; programs; protein structure; research study; solid state; solid state nuclear magnetic resonance

The improvement of RF sample probes is crucial to the development of high field biological solid state NMR. Unlike solution NMR, solid state NMR techniques require exposing the delicate biological sample to very high intensity radio frequency (RF) magnetic fields. Unless the electric field component E of these fields is minimized, the sample will very likely be heated to destruction before the experiment is completed. In Core B, we will apply the "low-E" technology that we have developed for double frequency widebore NMR to the important areas of triple resonance and more accessible standard bore magnets. We will also adapt these probes for the sample/flow applications that are a centerpiece of the proposal. The technology developed in this Core will not only be needed to carry out the scientific program described in the proposal, but it will broadly impact the ability of NMR spectroscopists to conduct protein structure and dynamics studies in the solid state. Any area of health and medicine that benefits from a better understanding of proteins may be affected.

射频样品探针的改进对于高场生物固态核磁共振的发展至关重要。 与溶液 NMR 不同，固态 NMR 技术需要将精致的生物样品暴露在非常强的环境中。 高强度射频 (RF) 磁场。除非这些场的电场分量E是 最小化，样品很可能在实验完成之前被加热至破坏。核心 B、我们将把我们为双频宽口径核磁共​​振开发的“低辐射”技术应用到 三重共振的重要领域和更容易接近的标准孔径磁铁。我们还将调整这些 用于样品/流量应用的探头是该提案的核心。 该核心开发的技术不仅需要执行所描述的科学计划 提案中，但它将广泛影响核磁共振波谱学家进行蛋白质结构和分析的能力 固态动力学研究。任何受益于更好的健康和医学领域 对蛋白质的理解可能会受到影响。