CONTROL OF DISEASE IN ZEBRAFISH RESEARCH FACILITIES

斑马鱼研究设施中的疾病控制

• 批准号: 6771841
• 负责人: Michael Lawrence Kent
• 金额: $ 17.69万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6771841
• 关键词: Microsporidia; Mycobacterium; animal colony; biological models; biomedical facility; comorbidity; diagnosis design /evaluation; disease /disorder prevention /control; epizootiology; fertility; health science research; microorganism classification; microorganism disease chemotherapy; parasite infection mechanism; polymerase chain reaction; stress; vertical transmission; virulence; zebrafish

DESCRIPTION (provided by applicant): The last several years have witnessed an explosion in the understanding of vertebrate development, largely based on work from a few model genetic organisms. The embryonic zebrafish is one of these new models, and many research grants are funded by National Institutes of Health and NIEHS to utilize this model. Studies of the embryology and genetics of zebrafish and the recent identification of thousands of genetic mutations have lead to this dramatic increase in the number of laboratories (now ca 250) using zebrafish to study the basic mechanisms of vertebrate developmental biology. Although zebrafish have become an extremely important research model, relatively little is known about the diseases that affect them when held in captivity. Indeed, severe health problems in zebrafish research colonies could seriously jeopardize many millions of dollars in research funding, and some research facilities have experienced devastating, acute mortalities in their zebrafish colonies. Moreover, persistent, but less severe, infections have plagued several facilities. As with other laboratory animals used in research, it is imperative to conduct studies with disease-free, healthy zebrafish. Unlike rodent models, there are no certified SPF stocks of zebrafish, and the investigators are just now developing sensitive diagnostic tests for the most serious pathogens. The major aim of the present study is to improve the health of zebrafish used in research facilities by elucidation of the pathogenesis and modes of transmission of the most important pathogens in this fish e.g., Pseudoloma (P.) neurophilia, a microsporidium of the central nervous system that is very prevalent in zebrafish, and Mycobacterium spp, the cause of fish tuberculosis. As P. neurophilia is extremely prevalent in zebrafish facilities, the investigators will also investigate its impact on fecundity. The investigators will improve polymerase chain reaction (PCR)-based diagnostic tests for these pathogens. With these tests in hand the applicants will elucidate epidemiological factors of these infections in zebrafish colonies. Results will be quickly distributed to the zebrafish community through Zebrafish International Network, and through peer-reviewed publications. In addition, the investigators have developed a Web-based fish health manual, and new information generated in the present study will be incorporated into this manual.

描述（由申请人提供）： 过去几年见证了对脊椎动物发育的理解爆炸，这在很大程度上基于一些模型遗传生物的工作。 胚胎斑马鱼是这些新模型之一，许多研究赠款都是由国立卫生研究院和NIEHS资助的。 斑马鱼的胚胎学和遗传学的研究以及最近对数千种遗传突变的鉴定导致了使用斑马鱼研究脊椎动物发育生物学的基本机制的实验室数量（现为CA 250）的急剧增加。 尽管斑马鱼已成为一个极为重要的研究模型，但对被囚禁时影响它们的疾病的了解相对较少。 确实，斑马鱼研究殖民地的严重健康问题可能会严重危害数百万美元的研究资金，一些研究机构在其斑马鱼殖民地中经历了毁灭性的急性死亡。 此外，持久但不太严重的感染困扰着几个设施。 与研究中使用的其他实验动物一样，必须使用无疾病，健康的斑马鱼进行研究。 与啮齿动物的模型不同，没有认证的斑马鱼库存，研究人员刚刚为最严重的病原体开发了敏感的诊断测试。 本研究的主要目的是通过阐明这种鱼类中最重要的病原体的发病机理和传播方式来改善研究设施中使用的斑马鱼的健康，例如，假神经虫（P.）神经嗜孢菌，这是中枢神经系统的微孢子虫，一种中枢神经系统的微孢子虫，在Zeprafish和mycobaccium and mycobactium s fisterium中非常普遍。 由于斑马鱼设施中的神经性疟原虫极为普遍，研究人员还将研究其对繁殖力的影响。 研究人员将改善这些病原体的基于聚合酶链反应（PCR）的诊断测试。 借助这些测试，申请人将阐明斑马鱼菌落中这些感染的流行病学因素。 结果将通过斑马鱼国际网络和同行评审的出版物快速分发给斑马鱼社区。 此外，研究人员已经开发了一份基于网络的鱼类健康手册，本研究中生成的新信息将纳入本手册中。