Control and Impacts of Diseases in Zebrafish Research Facilities

斑马鱼研究设施中疾病的控制和影响

• 批准号: 8721085
• 负责人: Michael Lawrence Kent
• 金额: $ 3.65万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8721085
• 关键词: Adult; Aging; Animal Model; Antibiotics; Back; Behavior; Biomedical Research; Bone Marrow Transplantation; Chronic; Clinical; Communities; Diagnosis; Diagnostic; Diagnostic Services; Diagnostic tests; Disease; Disease Outbreaks; Disinfectants; Drosophila genus; Duchenne muscular dystrophy; Embryo; Epidemiologic Studies; Female; Fertility; Fishes; Gamma Rays; Genus Mycobacterium; Grant; Hand; Health; Histology; Human; Immune; Impact evaluation; Individual; Infection; International; Laboratories; Laboratory Research; Lead; Longitudinal Studies; Malignant Neoplasms; Manuals; Methods; Microsporidia; Microsporidiosis; Microsporidium; Modeling; Molecular; Mycobacterium Infections; Neoplasm Transplantation; Online Systems; Organ; Ovary; Paraffin; Paraffin Tissue; Paramecium; Parasites; Pathogenesis; Patients; Pharmaceutical Preparations; Plague; PubMed; Publications; Reporting; Reproduction spores; Research; Research Personnel; Resources; Risk; Sentinel; Staining method; Stains; Surveys; Systemic infection; Taxonomy; Testing; Toxicology; United States National Institutes of Health; Variant; Virulent; Visceral; Work; Zebrafish; base; chemotherapy; cytokine; developmental genetics; egg; experience; feeding; immune function; improved; irradiation; killings; melanoma; mortality; mycobacterial; pathogen; research facility; tissue culture; tool; transmission process; ultraviolet irradiation; vector; web site

DESCRIPTION (provided by applicant): The benefit of using zebrafish (Danio rerio) models as part of an integrative approach to improve human health is being realized by the scientific community, with 800 laboratories, supported by some 735 grants from NIH using this fish model. As with any animal model, infections can compromise research by causing high mortalities and subclinical infections compromise research by inducing non-protocol induced variation. Third, Mycobacterium spp. that have been reported from zebrafish are all recognized human pathogens, and thus fish with these infections present human health concerns. Therefore, as with other models, it is imperative to conduct studies with disease-free, healthy zebrafish. Based on 12 years of diagnostics performed by the Zebrafish International Research Center (ZIRC) and research conducted in the last cycles of this grant, we can conclude the following: Mycobacterium spp. and infections by Pseudoloma neurophilia (Microsporidia) are very common in zebrafish research laboratories. Some research facilities have experienced devastating mortalities in their zebrafish colonies due to infections by virulent Mycobacterium spp. Persistent, but less severe, infections by other mycobacteria and Pseudoloma have also plagued several facilities. The modes of transmission of these pathogens have been elucidated, and PCR-based diagnostic tests are available. However, little is known about how subclinical infections impact research endpoints, which is important now as several researchers are now using zebrafish as models in long-term studies on human pathogens, toxicology, aging and behavior. The major aim of the present study is to improve the health of zebrafish used in research facilities by investigating efficacy of disinfectants and antibiotics to reduce or eliminae infections. Evaluation of impacts of chronic infections by M. chelonae and Pseudoloma on fecundity, behavior, and cytokine profiles is another aim. Zebrafish that are immunsuppressed by gamma radiation are important models for human cancer studies, and investigate the impacts of underlying Pseudoloma infections in these fish will be studied. Results will be quickly distributed to the zebrafish community through through publications and the ZIRC web based manual on zebrafish.

描述（由申请人提供）：使用斑马鱼（Danio Rerio）模型作为改善人类健康的综合方法的一部分的好处正在由科学界实现，有800个实验室，使用该鱼类模型从NIH提供了735个赠款。与任何动物模型一样，感染可以通过诱导非主体诱导的差异来损害高死亡率和亚临床感染来损害研究。第三，分枝杆菌属。斑马鱼报道的都是公认的人类病原体，因此带有这些感染的捕鱼引起了人类健康的关注。因此，与其他模型一样，必须使用无疾病，健康的斑马鱼进行研究。基于斑马鱼国际研究中心（ZIRC）进行的12年诊断和在本赠款的最后一个周期中进行的研究，我们可以得出以下结论：分枝杆菌属。斑马鱼研究实验室中，神经性神经瘤（微孢子虫）的感染非常常见。一些研究设施由于毒性分枝杆菌的感染而导致斑马鱼菌落的毁灭性死亡率。其他分枝杆菌和伪瘤的持久但不太严重的感染也困扰着几个设施。这些病原体的传播模式已经阐明，并且可以使用基于PCR的诊断测试。但是，对于亚临床感染如何影响研究终点，这一点很重要，因为现在一些研究人员现在使用斑马鱼作为人类病原体，毒理学，衰老和行为的长期研究的模型。本研究的主要目的是通过研究消毒剂和抗生素的功效来减少或消除感染，改善研究设施中使用的斑马鱼的健康。评估Chelonae M. chelonae和Pseudoloma对繁殖力，行为和细胞因子谱的影响的影响是另一个目的。伽马辐射免疫抑制的斑马鱼是人类癌症研究的重要模型，并研究了这些鱼类感染中潜在的伪瘤感染的影响。结果将通过出版物和基于Zirc Web的手册快速分发给斑马鱼社区。