Control and Impacts of Diseases in Zebrafish Research Facilities

斑马鱼研究设施中疾病的控制和影响

• 批准号: 8721085
• 负责人: Michael Lawrence Kent
• 金额: $ 3.65万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8721085
• 关键词: Adult; Aging; Animal Model; Antibiotics; Back; Behavior; Biomedical Research; Bone Marrow Transplantation; Chronic; Clinical; Communities; Diagnosis; Diagnostic; Diagnostic Services; Diagnostic tests; Disease; Disease Outbreaks; Disinfectants; Drosophila genus; Duchenne muscular dystrophy; Embryo; Epidemiologic Studies; Female; Fertility; Fishes; Gamma Rays; Genus Mycobacterium; Grant; Hand; Health; Histology; Human; Immune; Impact evaluation; Individual; Infection; International; Laboratories; Laboratory Research; Lead; Longitudinal Studies; Malignant Neoplasms; Manuals; Methods; Microsporidia; Microsporidiosis; Microsporidium; Modeling; Molecular; Mycobacterium Infections; Neoplasm Transplantation; Online Systems; Organ; Ovary; Paraffin; Paraffin Tissue; Paramecium; Parasites; Pathogenesis; Patients; Pharmaceutical Preparations; Plague; PubMed; Publications; Reporting; Reproduction spores; Research; Research Personnel; Resources; Risk; Sentinel; Staining method; Stains; Surveys; Systemic infection; Taxonomy; Testing; Toxicology; United States National Institutes of Health; Variant; Virulent; Visceral; Work; Zebrafish; base; chemotherapy; cytokine; developmental genetics; egg; experience; feeding; immune function; improved; irradiation; killings; melanoma; mortality; mycobacterial; pathogen; research facility; tissue culture; tool; transmission process; ultraviolet irradiation; vector; web site

DESCRIPTION (provided by applicant): The benefit of using zebrafish (Danio rerio) models as part of an integrative approach to improve human health is being realized by the scientific community, with 800 laboratories, supported by some 735 grants from NIH using this fish model. As with any animal model, infections can compromise research by causing high mortalities and subclinical infections compromise research by inducing non-protocol induced variation. Third, Mycobacterium spp. that have been reported from zebrafish are all recognized human pathogens, and thus fish with these infections present human health concerns. Therefore, as with other models, it is imperative to conduct studies with disease-free, healthy zebrafish. Based on 12 years of diagnostics performed by the Zebrafish International Research Center (ZIRC) and research conducted in the last cycles of this grant, we can conclude the following: Mycobacterium spp. and infections by Pseudoloma neurophilia (Microsporidia) are very common in zebrafish research laboratories. Some research facilities have experienced devastating mortalities in their zebrafish colonies due to infections by virulent Mycobacterium spp. Persistent, but less severe, infections by other mycobacteria and Pseudoloma have also plagued several facilities. The modes of transmission of these pathogens have been elucidated, and PCR-based diagnostic tests are available. However, little is known about how subclinical infections impact research endpoints, which is important now as several researchers are now using zebrafish as models in long-term studies on human pathogens, toxicology, aging and behavior. The major aim of the present study is to improve the health of zebrafish used in research facilities by investigating efficacy of disinfectants and antibiotics to reduce or eliminae infections. Evaluation of impacts of chronic infections by M. chelonae and Pseudoloma on fecundity, behavior, and cytokine profiles is another aim. Zebrafish that are immunsuppressed by gamma radiation are important models for human cancer studies, and investigate the impacts of underlying Pseudoloma infections in these fish will be studied. Results will be quickly distributed to the zebrafish community through through publications and the ZIRC web based manual on zebrafish.

描述（由申请人提供）：科学界正在认识到使用斑马鱼 (Danio rerio) 模型作为改善人类健康综合方法的一部分的好处，该实验室拥有 800 个实验室，并得到 NIH 约 735 项拨款的支持，使用该鱼模型。与任何动物模型一样，感染可能会导致高死亡率，从而影响研究，而亚临床感染则可能会引发非方案诱导变异，从而影响研究。第三，分枝杆菌属。据报道，斑马鱼感染的病毒都是公认的人类病原体，因此患有这些感染的鱼类会引起人类健康问题。因此，与其他模型一样，必须用无病、健康的斑马鱼进行研究。根据斑马鱼国际研究中心 (ZIRC) 12 年的诊断以及本次资助的最后一个周期中进行的研究，我们可以得出以下结论：分枝杆菌属。嗜神经假单胞菌（微孢子虫）感染在斑马鱼研究实验室中非常常见。由于有毒分枝杆菌的感染，一些研究机构的斑马鱼群体出现了毁灭性的死亡。其他分枝杆菌和假单胞菌的持续但不太严重的感染也困扰着一些设施。这些病原体的传播方式已被阐明，基于 PCR 的诊断测试也已可用。然而，人们对亚临床感染如何影响研究终点知之甚少，这一点现在很重要，因为一些研究人员现在使用斑马鱼作为人类病原体、毒理学、衰老和行为的长期研究模型。本研究的主要目的是通过研究消毒剂和抗生素减少或消除感染的功效来改善研究设施中使用的斑马鱼的健康。评估龟分枝杆菌和假单胞菌慢性感染对繁殖力、行为和细胞因子谱的影响是另一个目的。受到伽马辐射免疫抑制的斑马鱼是人类癌症研究的重要模型，我们将研究这些鱼潜在的假瘤感染的影响。结果将通过出版物和 ZIRC 基于斑马鱼的网络手册快速分发给斑马鱼社区。