Control and Impacts of Disease of Zebrafish in Research Facilities

研究设施中斑马鱼疾病的控制和影响

• 批准号: 10451091
• 负责人: Michael Lawrence Kent
• 金额: $ 11.78万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10451091
• 关键词: Administrative Supplement; Affect; Bacteria; Common Neoplasm; Communicable Diseases; Communities; Diagnosis; Diet; Disease; Etiology; Family Picornaviridae; Fishes; Funding; Genus Mycobacterium; Grant; Health; Human; Infection; International; Intestinal Cancer; Intestinal Neoplasms; Intestines; Laboratory Research; Link; Microbe; Microsporidiosis; Modeling; Monoclonal Antibody R24; Mus; Mycobacterium chelonae; Mycoplasma; Mycoplasma penetrans; Nematoda; Pathologic; Population; Research; Research Support; Resources; Role; Texas; United States National Institutes of Health; Universities; Work; Zebrafish; enteritis; experimental study; falls; graduate student; improved; microorganism; novel; pathogen; public health relevance; research facility; tool; transmission process

Project Summary/Abstract - Bridge The use of zebrafish (Danio rerio) as part of an integrative approach to improving human health is now second to mice in vertebrate models, with over 1,000 NIH grants using the model. Certain pathogens are very common in zebrafish facilities. The three most common diseases/infections of zebrafish in research laboratories are microsporidiosis, caused by Pseudoloma neurophilia, mycobacteriosis (caused by multiple species of Mycobacterium), and parasitic enteritis (caused by the nematode Pseudocapillaria tomentosa), and now we add Mycoplasma penetrans, the picornavirus ZPV to this list. Moreover, a transmissible intestinal cancer is also very common, and may be caused by Mycoplasma penetrans. Our proposed research investigate the role of Mycoplasma and other agents as causes of the intestinal tumors. We will also study pathologic effects of ZPV. We continue our work with Mycobacterium chelonae, focusing on maternal transmission and the effects of diet. We are not asking for support to study continue research on Pseudoloma and Pseudocapillaria as we believe we have accomplished the appropriate aims put forth in our previous grants funded by NIH ORIP. Proposed here is Bridge Funding to maintain our unique zebrafish vivarium so that we can soon initiate the above proposed research.

项目总结/摘要 - 桥梁 使用斑马鱼（Danio rerio）作为改善人类健康综合方法的一部分现已成为第二位 脊椎动物模型中的小鼠，有超过 1,000 项 NIH 拨款使用该模型。某些病原体非常 常见于斑马鱼设施中。研究中斑马鱼三种最常见的疾病/感染 实验室检查是微孢子虫病，由假性神经病引起，分枝杆菌病（由多种原因引起） 分枝杆菌属）和寄生性肠炎（由毛毛细毛线虫引起），以及 现在我们将穿透支原体、小核糖核酸病毒 ZPV 添加到此列表中。此外，一种可传播的肠道 癌症也很常见，可能是由穿透支原体引起的。我们提出的研究 研究支原体和其他病原体作为肠道肿瘤原因的作用。我们也会学习 ZPV 的病理作用。我们继续与龟分枝杆菌合作，重点关注母体 传播和饮食的影响。我们不寻求支持继续研究假瘤 和假毛细血管，因为我们相信我们已经实现了之前提出的适当目标 由 NIH ORIP 资助的赠款。这里建议提供桥梁资金来维持我们独特的斑马鱼饲养场 以便我们能够尽快启动上述建议的研究。

项目成果

Unusual fluorescent granulomas and myonecrosis in Danio rerio infected by the microsporidian pathogen Pseudoloma neurophilia.

被微孢子虫病原体假神经嗜神经菌感染的斑马鱼出现异常的荧光肉芽肿和肌肉坏死。

• DOI: 10.1089/zeb.2013.0933
• 发表时间: 2014
• 期刊: Zebrafish
• 影响因子: 2
• 作者: West,Kylie;Miles,Rodney;Kent,MichaelL;Frazer,JKimble
• 通讯作者: Frazer,JKimble

Viability of Pseudocapillaria tomentosa Eggs Exposed to Heat, Ultraviolet Light, Chlorine, Iodine, and Desiccation.

毛毛细线虫卵暴露于热、紫外线、氯、碘和干燥条件下的活力。

• DOI: 10.1089/zeb.2019.1736
• 发表时间: 2019
• 期刊: Zebrafish
• 影响因子: 2
• 作者: Kent,MichaelL;Watral,Virginia;Villegas,EricN;Gaulke,ChristopherA
• 通讯作者: Gaulke,ChristopherA

Detection of the parasitic nematode, Pseudocapillaria tomentosa, in zebrafish tissues and environmental DNA in research aquaria.

检测研究型水族箱中斑马鱼组织和环境 DNA 中的寄生线虫 Pseudocapillaria tomentosa。

• DOI: 10.1111/jfd.13220
• 发表时间: 2020
• 期刊: Journal of fish diseases
• 影响因子: 2.5
• 作者: Norris,Lauren;Lawler,Noah;Hunkapiller,Andree;Mulrooney,DonnaM;Kent,MichaelL;Sanders,JustinL
• 通讯作者: Sanders,JustinL

Luna stain, an improved selective stain for detection of microsporidian spores in histologic sections.

• DOI: 10.3354/dao02346
• 发表时间: 2011-06-16
• 期刊: Diseases of aquatic organisms
• 影响因子: 1.4
• 作者: Peterson TS;Spitsbergen JM;Feist SW;Kent ML
• 通讯作者: Kent ML

Pseudoloma neurophilia Infection Combined with Gamma Irradiation Causes Increased Mortality in Adult Zebrafish (Danio rerio) Compared to Infection or Irradiation Alone: New Implications for Studies Involving Immunosuppression.

与单独感染或辐照相比，假性神经细胞增多症感染与伽马辐照相结合会导致成年斑马鱼（斑马鱼）死亡率增加：涉及免疫抑制研究的新含义。

• DOI: 10.1089/zeb.2015.1223
• 发表时间: 2016
• 期刊: Zebrafish
• 影响因子: 2
• 作者: Spagnoli,SeanT;Sanders,JustinL;Watral,Virginia;Kent,MichaelL
• 通讯作者: Kent,MichaelL