Functional Microbiomics, Inflammation and Pathogenicity

功能微生物组学、炎症和致病性

• 批准号: 10797084
• 负责人: Richard J Lamont
• 金额: $ 25万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797084
• 关键词: Alzheimer' s Disease; Animals; Area; Award; Bioinformatics; Biological; Biological Assay; Biological Sciences; Cells; Cytometry; Dedications; Development; Disease; Faculty; Funding; Germ-Free; Goals; Image; Inflammation; Inflammatory; Interdisciplinary Study; Laboratories; Malignant Neoplasms; Mentors; Methods; Molecular; Pathogenesis; Pathogenicity; Periodontal Diseases; Phase; Process; Proteomics; Qualifying; Research; Research Personnel; Research Support; Resolution; Role; Technology; Tissues; United States National Institutes of Health; Whole Organism; animal facility; human disease; imaging capabilities; imaging modality; inflammatory marker; instrument; intravital microscopy; microbiome; microbiome research; microbiome sequencing; microbiota; multi-photon; novel; novel therapeutics; programs

ABSTRACT The Primary goal of the “Functional Microbiomics, Inflammation and Pathogenicity” CoBRE at the UofL is to establish a thematic multidisciplinary research center for the study of the interface between the microbiome, inflammation and pathogenicity. In the last five years, we assembled a cadre of junior investigators with complementary expertise and outstanding potential and have partnered these project leaders with well-qualified and dedicated mentors. An integrated set of complementary projects made novel connections between inflammatory processes and bacterial pathogenicity to establish the basis for the development of novel therapeutics and delivery methods. This has led to successful graduation of five junior faculty with NIH R01 awards and three of these investigators are still at UofL and are transitioning into mentoring roles while establishing their own strong scientific programs. Thus far, research in their laboratories has been supported by a Functional Microbiomics Core that provides germ free animal facilities, anaerobic culture capability, microbiome sequencing and bioinformatics, and assay of inflammatory markers. For many of our junior faculty and their mentors, there is now increasing demand for the ability to image inflammation deeper into tissues in live animals and spatial resolution within the tissues has become an important requirement for many projects. Thus, for the phase II of the FMIP CoBRE, we have made significant commitment to expand our imaging capabilities by adding Multiphoton Intravital Microscopy (MP-IVM), a well-established method for imaging of living, intact biological tissues through the whole organisms to our repertoire of technologies in FMC. We propose to further expand this area by purchasing a CellScape_Chip Cytometry Instrument from Canopy Biosciences. Chip Cytometry can multiplex the spatial proteomics at single cell resolution. This supplement application is requesting partial funding from the NIH to acquire and establish Chip Cytometry at the UofL that will support the research of large number of faculty working in the areas of microbiota, inflammation, and pathogenesis.

抽象的 UOFL的“功能性微生物学，炎症和致病性”的主要目标是 建立一个主题多学科研究中心，以研究微生物组之间的界面研究中心 炎症和致病性。在过去的五年中，我们与 完全专业知识和杰出的潜力，并将这些项目负责人与合格的合伙人合作 和敬业的导师。一组集成的互补项目在之间建立了新的联系 炎症过程和细菌的致病性，以建立发展的基础 治疗和交付方法。这导致了NIH R01的五名初级教师的成功毕业 奖项和其中三名调查人员仍在UOFL，并且正在转变为心理角色，而 建立自己的强大科学计划。远处的实验室研究得到了支持 提供无菌动物设施的功能性微生物核心，厌氧培养能力， 微生物组测序和生物信息学，以及炎症标记的测定。对于我们的许多初级教师 和他们的导师，现在对成像炎症更深入组织的能力的需求不断增加 动物和组织内的空间分辨率已成为许多项目的重要要求。那， 对于FMIP COBRE的第二阶段，我们做出了重大承诺，以扩大成像功能 添加多光子插入显微镜（MP-IVM），这是一种完善的成像，完整的方法 通过整个组织的生物组织到我们在FMC中的技术曲目。我们建议进一步 通过从顶篷生物科学购买CellScape_Chip细胞仪仪器来扩展该区域。芯片 细胞仪可以在单细胞分辨率下多重多重空间蛋白质组学。此补充申请要求 NIH的部分资金以获取并在UOFL上建立芯片细胞仪，这将支持研究 在微生物群，感染和发病机理领域工作的大量教师。