Functional Microbiomics, Inflammation and Pathogenicity

功能微生物组学、炎症和致病性

• 批准号: 10797084
• 负责人: Richard J Lamont
• 金额: $ 25万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797084
• 关键词: Alzheimer' s Disease; Animals; Area; Award; Bioinformatics; Biological; Biological Assay; Biological Sciences; Cells; Cytometry; Dedications; Development; Disease; Faculty; Funding; Germ-Free; Goals; Image; Inflammation; Inflammatory; Interdisciplinary Study; Laboratories; Malignant Neoplasms; Mentors; Methods; Molecular; Pathogenesis; Pathogenicity; Periodontal Diseases; Phase; Process; Proteomics; Qualifying; Research; Research Personnel; Research Support; Resolution; Role; Technology; Tissues; United States National Institutes of Health; Whole Organism; animal facility; human disease; imaging capabilities; imaging modality; inflammatory marker; instrument; intravital microscopy; microbiome; microbiome research; microbiome sequencing; microbiota; multi-photon; novel; novel therapeutics; programs

ABSTRACT The Primary goal of the “Functional Microbiomics, Inflammation and Pathogenicity” CoBRE at the UofL is to establish a thematic multidisciplinary research center for the study of the interface between the microbiome, inflammation and pathogenicity. In the last five years, we assembled a cadre of junior investigators with complementary expertise and outstanding potential and have partnered these project leaders with well-qualified and dedicated mentors. An integrated set of complementary projects made novel connections between inflammatory processes and bacterial pathogenicity to establish the basis for the development of novel therapeutics and delivery methods. This has led to successful graduation of five junior faculty with NIH R01 awards and three of these investigators are still at UofL and are transitioning into mentoring roles while establishing their own strong scientific programs. Thus far, research in their laboratories has been supported by a Functional Microbiomics Core that provides germ free animal facilities, anaerobic culture capability, microbiome sequencing and bioinformatics, and assay of inflammatory markers. For many of our junior faculty and their mentors, there is now increasing demand for the ability to image inflammation deeper into tissues in live animals and spatial resolution within the tissues has become an important requirement for many projects. Thus, for the phase II of the FMIP CoBRE, we have made significant commitment to expand our imaging capabilities by adding Multiphoton Intravital Microscopy (MP-IVM), a well-established method for imaging of living, intact biological tissues through the whole organisms to our repertoire of technologies in FMC. We propose to further expand this area by purchasing a CellScape_Chip Cytometry Instrument from Canopy Biosciences. Chip Cytometry can multiplex the spatial proteomics at single cell resolution. This supplement application is requesting partial funding from the NIH to acquire and establish Chip Cytometry at the UofL that will support the research of large number of faculty working in the areas of microbiota, inflammation, and pathogenesis.

抽象的 伦敦大学“功能微生物组学、炎症和致病性”CoBRE 的主要目标是 建立一个专题多学科研究中心，研究微生物组之间的界面， 在过去的五年里，我们组建了一支初级研究人员队伍。 互补的专业知识和突出的潜力，并与这些项目领导者建立了良好的合作伙伴关系 和专门的导师，一套完整的互补项目在两者之间建立了新颖的联系。 炎症过程和细菌致病性，为新型药物的开发奠定基础 这使得 NIH R01 的五名初级教师成功毕业。 奖项和其中三名研究人员仍在伦敦大学，并正在过渡到指导角色，同时 到目前为止，他们实验室的研究得到了以下机构的支持： 功能性微生物组学核心，提供无菌动物设施、厌氧培养能力、 微生物组测序和生物信息学，以及炎症标记物的测定 对于我们许多初级教师来说。 和他们的导师，现在对活体组织中更深入的炎症成像的需求越来越大 动物和组织内的空间分辨率已成为许多项目的重要要求。 对于 FMIP CoBRE 的第二阶段，我们做出了重大承诺，通过以下方式扩展我们的成像能力： 添加多光子活体显微镜 (MP-IVM)，这是一种成熟的活体完整成像方法 生物组织到整个生物体，我们建议进一步发展 FMC 技术。 通过购买 Canopy Biosciences Chip 的 CellScape_Chip 细胞计数仪来扩展该领域。 细胞计数可以在单细胞分辨率下多重分析空间蛋白质组学。 美国国立卫生研究院 (NIH) 提供部分资金，用于在伦敦大学收购和建立芯片细胞术，以支持以下研究 大量教师从事微生物群、炎症和发病机制领域的工作。