Liquid cartilage for arthroscopy

关节镜用液体软骨

• 批准号: 10649312
• 负责人: Kyriacos A Athanasiou
• 金额: $ 19.77万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10649312
• 关键词: Address; Allogenic; American; Animal Model; Animals; Appearance; Arthroscopy; Atmospheric Pressure; Autopsy; Biochemical; Biochemistry; Biological Assay; Biomechanics; Cartilage; Cells; Characteristics; Chondrocytes; Collaborations; Collagen; Data; Defect; Dryness; Exhibits; Extracellular Matrix; Femur; Filler; Hemorrhage; Histologic; Human; Hyaline Cartilage; Iatrogenesis; Joints; Knee; Knee joint; Left; Lesion; Liquid substance; Mechanics; Miniature Swine; Modeling; Monitor; Operative Surgical Procedures; Orthopedics; Phase; Phenotype; Procedures; Production; Proteomics; Pump; Research; Safety; Science; Series; Surface; Surgeon; Surveys; Techniques; Technology; Testing; Thick; Time; Tissue Engineering; Tissues; Viscosity; Visualization; Weight-Bearing state; Width; Work; articular cartilage; cartilage repair; design; efficacy evaluation; healing; in vivo; lubricin; preservation; pressure; repaired; response; rib bone structure; tissue culture

PROJECT SUMMARY This multi-PI proposal is a collaboration among tissue engineers and an orthopaedic surgeon to develop a liquid cartilage filler, called chondrogel, that would be efficacious in repairing gouge and focal defects of the knee using an arthroscopy approach. Chondrogel is formed using extensively passaged, allogeneic rib chondrocytes that have retained their ability to form mechanically robust neocartilage that is phenotypically akin to articular cartilage (e.g., production of collagen II and lubricin, absence of collagen I and X). Preliminary data show that chondrogel fills gouge defects in explant culture and also in vivo after 16wks in the minipig knee. We have also developed arthroscopic techniques for the minipig. In this proposal, we will show that, in addition to gouge defects, chondrogel will be effective in healing focal defects as well when administered arthroscopically. This will be achieved in two aims. In Aim 1, we will examine chondrogel 1) to show that, in addition to 20x1mm gouge defects, chondrogel is capable of filling focal defects 5mm in dia. in explant culture (Aim 1, Phase I), and 2) to find the maximum arthroscopic intraarticular pressure where chondrogel is still retained in gouge and focal defects (Aim 1, Phase II). In Aim 2, we will determine if chondrogel heals gouge and focal articular cartilage defects in the minipig using arthroscopy. Because chondrogel has been designed based on surgeon surveys and because it is based on rigorous science demonstrating its ability to form cartilage, successful completion of this proposal would allow the use of arthroscopy to treat cartilage defects.

项目摘要 该多PI的建议是组织工程师和骨科医生之间的合作，以开发液体 软骨填充剂，称为软骨盖，可以有效地修复膝盖的腹膜和局灶性缺陷 关节镜检查方法。软骨盖是使用广泛传递的同种异体肋骨软骨细胞形成的 保留了它们形成机械稳健的新能力的能力，在表型上类似于关节软骨 （例如，产生胶原蛋白II和润滑剂，缺乏胶原蛋白I和X）。初步数据显示了软骨盖 在Minipig膝盖中16周后，填充了外植物文化中的宫颈缺陷，并在体内填充体内。我们也发展了 小型运动的关节镜技术。在此提案中，我们还将证明，除了腹腔缺陷外， 在关节镜下给药时，软骨盖也将有效地治愈局灶性缺陷。这将是 在两个目标中实现。在AIM 1中，我们将检查Chondrogel 1）表明，除了20x1mm gouge缺陷外， Chondrogel能够填补DIA的局灶性缺陷。在Epplant文化（目标1，I阶段）和2） 最大关节镜内压，其中软骨凝胶仍保留在宫颈和局灶性缺陷中（AIM 1，第二阶段）。在AIM 2中，我们将确定软骨盖是否愈合在 Minipig使用关节镜检查。因为Chondrogel是根据外科医生的调查而设计的，因为 基于严格的科学，证明了其形成软骨的能力，成功完成了该建议 将允许使用关节镜治疗软骨缺陷。