ENHANCEMENT OF THE CPRC-SPF RHESUS MONKEY PROGRAM

加强 CPRC-SPF 恒河猴计划

• 批准号: 8693041
• 负责人: CARLOS A SARIOL
• 金额: $ 246.71万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8693041
• 关键词: Acquired Immunodeficiency Syndrome; Animal Housing; Animals; Area; Biological Assay; Biomedical Research; Birth; Breeding; Caribbean region; Characteristics; Communities; Country; Development; Funding; Genetic; Genetic Heterogeneity; Genetic Markers; Grant; Growth; Housing; Human; Inbreeding; Macaca; Macaca mulatta; Medical; Modeling; Monitor; Monkeys; National Center for Research Resources; Papovaviridae; Polyomavirus; Primates; Provider; Puerto Rico; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Science; Serologic tests; Serological; Simian T-lymphotropic virus 1; Simian virus 40; Specific Pathogen Frees; Structure; Surveys; Techniques; Testing; United States National Institutes of Health; Universities; Virus; cohort; germ free condition; meetings; offspring; programs; vector vaccine; virology

DESCRIPTION (provided by applicant): The objective of this proposal is to maintain and enhance the specific pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Caribbean Primate Research Center (CPRC). This program consists of two SPF colonies (one closed and the other open). While the closed colony growths only by births, the open colony increases its size not only by births, but also by incorporating SPF candidates to its cohort. The closed colony (referred as to the U24 SPF colony) will consist of a minimum of 652 animals, and the open colony (referred as to SPF-A) will start with 468 animals. Thus, the founding stock of these genetically-characterized Indian-origin monkey colonies will be donated by CPRC and will consist of approximately 1,120 SPF (Herpes B, STLV-1, SIV and SRV-free) rhesus macaques. About 82 (7%) of the U24 SPF animals will be also free of the papovavirus SV40. Thus, this program will consist of two U24 SPF sub colonies: one SV40-free and the other SV40 positive. Animals from these SPF sub colonies will be maintained in well-defined and separated areas, will be housed in reproduction corncribs, and will be quarterly tested for the presence of the above mentioned target viruses. All SPF colonies will be closely monitored by well-established genetic and virology techniques and assays. A panel of highly polymorphic genetic markers will be used to characterize the U24 SPF and SPF-A colonies and to optimize the structure of breeding groups, maximizing the level of their genetic heterogeneity. At the end of this four year grant, the SPF-A colony will be closed. The entire program will produce a total estimated offspring of more than 1,350 SPF rhesus macaques, of which 96 will be also SV40 negative. It is expected that the SPF colonies of this program will make available to PHS-funded grants about 300 animals/year and that it will attain self-sufficiency by the end of this grant. CPRC SPF macaques will help meet the increased research demand that exists for these animals. Our Center will continue to make significant contributions to AIDS and to other research programs by providing high quality, healthy, and genetically-characterized SPF rhesus monkeys to NIH-sponsored research initiatives.

描述（由申请人提供）：本提案的目的是维持和加强加勒比灵长类动物研究中心（CPRC）的无特定病原体（SPF）恒河猴供应和繁殖群计划。该程序由两个 SPF 菌落组成（一个封闭，另一个开放）。封闭群落仅通过出生来增长，而开放群落不仅通过出生来扩大规模，还通过将 SPF 候选者纳入其群体来扩大其规模。封闭群落（称为 U24 SPF 群落）将至少由 652 只动物组成，而开放群落（称为 SPF-A）将由 468 只动物组成。因此，这些具有遗传特征的印度猴群的创始种群将由 CPRC 捐赠，并将由大约 1,120 只 SPF（不含疱疹 B、STLV-1、SIV 和 SRV）的恒河猴组成。大约 82 只 (7%) 的 U24 SPF 动物也不会感染乳多空病毒 SV40。因此，该计划将由两个 U24 SPF 亚菌落组成：一个不含 SV40，另一个含有 SV40 阳性。来自这些 SPF 亚群的动物将被饲养在明确且分开的区域中，将被安置在繁殖玉米笼中，并且每季度进行一次上述目标病毒是否存在的测试。所有 SPF 菌落都将通过完善的遗传和病毒学技术及检测进行密切监测。一组高度多态性遗传标记将用于表征 U24 SPF 和 SPF-A 菌落，并优化育种群体的结构，最大限度地提高其遗传异质性水平。在这四年的拨款结束时，SPF-A 群体将被关闭。整个计划预计将产生总计超过 1,350 只 SPF 恒河猴的后代，其中 96 只也是 SV40 阴性。预计该计划的 SPF 群体每年将向 PHS 资助的赠款提供约 300 只动物，并在赠款结束时实现自给自足。 CPRC SPF 猕猴将有助于满足对这些动物日益增长的研究需求。我们中心将继续为 NIH 资助的研究项目提供高质量、健康且具有遗传特征的 SPF 恒河猴，为艾滋病和其他研究项目做出重大贡献。