MHC Genetic Typing

MHC基因分型

• 批准号: 10447032
• 负责人: CARLOS A SARIOL
• 金额: $ 28.18万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10447032
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Address; Alleles; Animal Husbandry; Animals; Arizona; Back; Biochemistry; Biological Models; Biomedical Research; Breeding; Caribbean region; Caring; Cellular Immunity; Collaborations; Communities; DNA laboratory; Databases; Development; Disease; Disease Progression; Ensure; Excision; Foundations; Funding; Generations; Genetic; Genetic Heterogeneity; Genetic Variation; Genome; Goals; HIV; Health; Heterozygote; Human; Immune; Immune response; Inbreeding; Individual; Institution; International; Island; Macaca; Macaca mulatta; Maintenance; Major Histocompatibility Complex; Modeling; Monkeys; Natural Sciences; Physiological; Physiology; Population; Population Genetics; Primates; Production; Proteins; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Roundup; SIV; Safety; Schools; Shapes; Stratification; Testing; Time; United States National Institutes of Health; Universities; Virus; animal colony; base; computerized; design; driving force; genetic analysis; genetic pedigree; germ free condition; human disease; human subject; improved; intergenerational; mathematical sciences; microbicide; nonhuman primate; operation; programs; relational database; research and development; research facility; therapeutic development

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. This specific core is designed to continue our current efforts to maintain a genetically diverse, MHC-defined SPF Indian-origin rhesus macaque colony for use in AIDS-related research. In 2015, the KDL began its collaboration with the current U42 to design and improve the SPF breeding colony to enhance the efficiency of colony maintenance as well as to maximize genetic heterogeneity of the colony animals. Since then we have been constructing multigenerational SPF pedigrees at the CPRC which can be traced back to founders in the Cayo Santiago island population.

项目概要/摘要： 此应用程序的主要目标是维持加勒比海地区的无特定病原体 (SPF) 计划 灵长类动物研究中心 (CPRC) 通过运营、管理、兽医护理、病毒学支持 MHC 分型以及我们综合遗传管理计划的改进。一个独特的特点是 CPRC 动物有利于 SPF 群体的成功建立和可持续发展，因为这些动物是 天然不含 SIV 和 SRV-D（待检测的四种病毒中的两种）。尽管如此，为了安全并根据需要 对于 SPF 计划，我们继续对所有四种病毒（B 病毒、STLV、SIV 和 SRV-D）进行系统测试。这 该计划提供具有明确遗传和病毒学背景的纯印度产恒河猴。 群体遗传分析显示，SPF 群体随着时间的推移一直保持遗传同质性 与其非 SPF 创始人相比，具有足够的杂合性和最小的分层。遗传 使用本文中描述的遗传管理策略将在 SPF 群体中维持变异 应用。遗传管理方法将允许富集所需的 MHC 标记 用于生物医学研究，同时保持遗传多样性。这个特定的核心旨在延续我们当前的 努力维持遗传多样性、MHC 定义的 SPF 印度原产恒河猴群体，用于 艾滋病相关研究。 2015年，KDL开始与现役U42合作，设计和改进 SPF繁殖群体，提高群体维持效率并最大化遗传 群体动物的异质性。从那时起，我们一直在构建多代 SPF 谱系 CPRC 的创始人可以追溯到圣地亚哥岛的居民。