Tai Chi and Gut Microbiota in Metabolic Osteoarthritis

太极拳和代谢性骨关节炎中的肠道微生物群

• 批准号: 10354621
• 负责人: CHENCHEN WANG
• 金额: $ 26.62万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10354621
• 关键词: 16S ribosomal RNA sequencing; Address; Adult; Affect; Age; American; Analysis of Variance; Arthritis; Behavioral; Biology; Body mass index; Clinical; Clinical Trials; Cohort Studies; Complex; DNA; Degenerative polyarthritis; Discriminant Analysis; Disease; Ecology; Education; Educational Intervention; Exercise; Exhibits; Future; Gender; Goals; Health Care Costs; Immune system; Individual; Intervention; Joints; Knee Osteoarthritis; Knowledge; Link; Measures; Mediating; Medical Care Costs; Meditation; Metabolic; Metabolic syndrome; Mind-Body Intervention; Moderate Exercise; Moods; Movement; Multivariate Analysis; Natural History; Neuraxis; Outcome; Pain; Participant; Pathogenesis; Pathway interactions; Patients; Persistent pain; Procedures; Public Health; Quality of life; Randomized; Relaxation; Research; Rheumatology; Role; Sampling; Severities; Streptococcus; Tai Ji; Testing; Therapeutic; Vegan Diet; Western Ontario and McMaster Universities Arthritis Index; attentional control; beta diversity; body-mind; clinical efficacy; clinical implementation; college; design; disability; disease phenotype; effective intervention; experience; follow-up; gut microbiome; gut microbiota; gut-brain axis; hypothalamic-pituitary-adrenal axis; illness length; immune activation; improved; innate immune pathways; innovation; insight; knee pain; metabolic phenotype; metagenomic sequencing; microbial; microbiome; microbiota; microbiota profiles; mind body interaction; osteoarthritis pain; pain processing; population based; psychosocial; randomized trial; response; stress reduction; systemic inflammatory response; theories

PROJECT SUMMARY Osteoarthritis (OA) is a major public health problem and leads to long-term pain, disability, and huge healthcare costs, for which there is a substantial therapeutic gap. The closely linked manifestations of OA with metabolic syndrome and its shared pathophysiological basis in activation of systemic inflammation through innate immune pathways, have predicated recognition of a highly prevalent ‘metabolic’ phenotype of OA that is associated with persistent pain. Consonant with metabolic syndrome, there is evidence for the interaction of the gut microbiome with occurrence and severity of OA pain that is mediated through innate immune activation by gut microbial flora. A large population-based cohort study further demonstrates an association of alterations in gut microbiota, specifically an increase in the relative abundance and quantity of Streptococcus species with osteoarthritis-related knee pain. These observations suggest that opportunities may exist to influence the natural history of OA by targeting the microbiome; and to better understand the biology of effective interventions of OA by studying their effects on the microbiome in the context of pain pathways. Randomized trials have demonstrated that Tai Chi, a multi-dimensional mind-body exercise, shows benefits for OA for 3 to 12 months or longer. However, limited knowledge of the underlying mechanisms has restricted further optimization of the clinical efficacy and implementation of this promising mind-body therapy. Our long-term goal is to elucidate the mechanistic basis of the effect of Tai Chi in treating OA pain. The aim of this exploratory study is to determine the effect of Tai Chi mind-body exercise on gut microbiota in patients with metabolic OA and estimate the effect size. We will randomize 80 eligible individuals who meet the American College of Rheumatology criteria for knee OA into Tai Chi or wellness education interventions for 12 weeks. Using 16S rRNA amplicon sequencing of fecal DNA samples we will compare the changes in gut microbiota profiles (composition, relative and differential abundance, alpha and beta diversity) using 16S rRNA gene amplicon sequencing, and quantity of Streptococcus species using quantitative PCR10, as well as their association with clinical outcomes. This proposal will provide crucial preliminary knowledge of the mechanism underlying Tai Chi mind-body therapy for metabolic OA. These results are necessary to guide the design of future studies to elucidate mechanistic interactions between Tai Chi, gut microbiota, pain and behavioral pathways underlying the potential disease-modifying role of mind-body approaches, ultimately advancing arthritis treatment.

项目摘要 骨关节炎（OA）是一个主要的公共卫生问题，导致长期疼痛，残疾和巨大的医疗保健费用l terapeutic GAP与代谢综合征紧密相关的表现。 ，具有与代谢综合征辅音相关的SSSSSSSSSSSSSSSSS，有证据表明发生了ICROBIOME，而OA疼痛是伴随肠道微生物菌群的介导的雷亚疼痛。这些观察结果的丰度和数量表明，这些观察结果可以通过靶向微生物组来实现OA的自然历史； 随机试验证明了泰国人的心理练习，这表明了OA的好处3至12个月或更长时间。治疗。 Tai Chi或健康的肠道微生物剖面（相对和差异丰度，Alpha和Beta多样性）的变化，使用16S g，以及使用定量PCR10的链球菌物种的数量，以及与临床临床效果的关联。 Tai Chi的机制用于代谢OA的思维体疗法。