Multi-Omics Core C

多组学核心 C

• 批准号: 10555124
• 负责人: Kelly Hyndman
• 金额: $ 34.63万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10555124
• 关键词: 16S ribosomal RNA sequencing; ATAC-seq; Acetylation; Address; Adult; Animals; Applications Grants; Basic Science; Bioinformatics; Biometry; Cell Nucleus; Cells; Chromatin; Chromatin Structure; Clinical; Cost Savings; DNA; DNA Methylation; DNA Sequencing Facility; Data; Data Analyses; Data Collection; Deposition; Ensure; Environment; Experimental Designs; Genes; Genomics; Goals; Histone Deacetylase; Human; Institution; Kidney; Life; Link; Liquid Chromatography; Lysine; Mass Spectrum Analysis; Mediating; Methods; Preparation; Proteins; Proteomics; Protocols documentation; Quality Control; RNA; Reagent; Regulation; Reproducibility; Resolution; Risk; Risk Factors; Rodent; Sampling; Standardization; Structure; Techniques; Technology; Testing; Tissues; Transcriptional Regulation; Volatile Fatty Acids; Work; bench to bedside; cardiovascular disorder risk; data management; data submission; early life stress; epigenetic regulation; epigenome; epigenomics; experience; experimental study; genome-wide analysis; gut microbiome; innovation; innovative technologies; member; metabolome; metabolomics; methylome; microbial; microbiome; microbiome research; multiple omics; new technology; repository; resilience; sample collection; single nucleus RNA-sequencing; synergism; tandem mass spectrometry; technology platform; transcriptome; transcriptome sequencing; transcriptomics

MULTI-OMICS CORE C SUMMARY This PPG is addressing an important hypothesis that early life stress (ELS) leads to reprogramming throughout the body resulting in an increased risk of developing CVD in adulthood. The mechanistic links between ELS and CVD and resilience in adulthood is not established. Epigenetic regulation of transcription or the gut microbiome and microbial-derived factors are hypothesized to be sensitive to ELS and may be modifiable to mediate resiliency. The primary goal and function of the Multi-omics Core (Core C) is to provide start-of-the-art processing of samples for determination of transcriptomes, DNA methylomes, DNA chromatin accessibility, metabolomes, and microbiomes required by all Projects. The objective of the Core is to provide consistency and rigor in the multi-omics experiments and provide innovative technologies to meet the goals of the Projects. The specific aims of Core C are: Aim 1 Transcriptomic and epigenomic determination. The epigenome is an important connection between the environment and the genes expressed in each cell (transcriptomes) and it is regulated by DNA methylation and chromatin structure (accessibility). Core C will facilitate determination of transcriptomes, and epigenome global DNA methylation and chromatin accessibility for the Projects. Aim 2: Microbiome and metabolite profiling. In this PPG, it is hypothesized that ELS results in gut microbial diversity changes that result in significant alterations to circulating short chain fatty acids (SCFA) or other metabolites. Core C will prepare samples for microbiome 16S sequencing for identifying and quantifying microbial diversity. The Core will also prepare samples for SCFA and untargeted metabolite determination by mass spectrometry. Aim 3: Evaluating new technologies, optimization while promoting fiscal responsibility. In this era of multi-omics, technologies are rapidly evolving. Thus, to provide the Projects with the latest technologies to test their hypotheses, Core C will evaluate new technological platforms. Core C will optimize all protocols for sample preparation for the multi-omics approaches listed above. By centralizing the mutli-omic sample preparation in Core C, we will purchase kits and reagents in bulk leading to substantial cost savings. Core C will interact directly with all Projects, receive clinical samples from Core B, and work directly with Core A for sample identification, curating, data deposition and analyses, and data management. Core A will complete all bioinformatics and biostatistical analyses for data generated in Core C. The approaches used in Core C ensure that the PPG is using the latest technologies for determining mechanisms of ELS induced cardiovascular disease risk with a bench to beside approach.

多词核心C摘要 该PPG正在解决一个重要的假设，即早期生活压力（ELS）导致整个过程中重新编程 身体导致成年后发生CVD的风险增加。 EL和EL之间的机械联系 CVD和成年期间的弹性尚未确定。转录或肠道微生物组的表观遗传调节 假设微生物衍生的因素对EL敏感，并且可以修改以介导 弹性。多摩斯核心（核心C）的主要目标和功能是提供启动处理 用于测定转录组，DNA甲基组，DNA染色质可及性，代谢组，代谢组的样品的样品 和所有项目都需要的微生物组。核心的目的是在 多派对实验并提供创新的技术来满足项目的目标。具体目标 核心的C为：AIM 1转录组和表观基因组学测定。表观基因组很重要 环境与每个细胞中表达的基因之间的连接（转录组），并受到调节 通过DNA甲基化和染色质结构（可访问性）。核心C将促进转录组的确定， 以及这些项目的表观基因组全球DNA甲基化和染色质的可及性。目标2：微生物组和 代谢物分析。在此PPG中，假设EL会导致肠道微生物多样性变化， 导致循环短链脂肪酸（SCFA）或其他代谢产物的重大改变。 Core C Will 准备微生物组16S测序的样品，以识别和量化微生物多样性。核心 还将通过质谱法制备用于SCFA和非靶向代谢物测定的样品。目标3： 评估新技术，优化，同时促进财政责任。在这个多词时代， 技术正在迅速发展。因此，为项目提供最新技术来测试他们的 假设Core C将评估新的技术平台。核心C将优化样本的所有协议 准备上面列出的多词方法的准备。通过将utli-omic样品制备集中在 Core C，我们将大量购买套件和试剂，从而节省大量成本。核心C将直接相互作用 在所有项目中，从Core B接收临床样本，并直接与Core A合作以进行样本识别， 策划，数据沉积和分析以及数据管理。核心A将完成所有生物信息学和 对核心C中生成的数据的生物统计分析。核心C中使用的方法确保PPG为 使用最新技术来确定EL诱导心血管疾病风险的机制 长凳在接近旁边。