In Vivo Animal and Human Studies Core

体内动物和人类研究核心

• 批准号: 9978789
• 负责人: CHUNG OWYANG
• 金额: $ 16.55万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978789
• 关键词: 3-Dimensional; Acute; Animal Model; Animals; Basic Science; Biopsy; Cell Separation; Cellular biology; Chronic; Clinical; Clinical Research; Consultations; Data; Development; Digestive System Disorders; Disease; Education; Electroporation; Endoscopy; Ensure; Epithelial Cells; Equipment; Experimental Designs; Feedback; Funding; Future; Gastrointestinal tract structure; Growth; Health Services Accessibility; Homeostasis; Human; Human Resources; In Vitro; Individual; Infrastructure; Intervention; Investigation; Liquid substance; Measurement; Michigan; Molecular; Mucous Membrane; Mus; Online Systems; Organ; Organoids; Peer Review; Physiological; Preparation; Publications; Research; Research Design; Research Personnel; Resources; Sample Size; Sampling; Services; Small Interfering RNA; Specimen; Statistical Data Interpretation; Techniques; Technology; Time; Tissue Banks; Tissues; Translating; Translations; Universities; base; biobank; body system; cell dimension; cellular imaging; clinical application; data management; design; gastrointestinal; human model; human pluripotent stem cell; imaging study; in vitro Model; in vivo; induced pluripotent stem cell; instrument; member; molecular imaging; new technology; organizational structure; power analysis; programs; repository; statistics; therapeutic target; user-friendly

The objective of the In Vivo Animal and Human Studies core is to provide a venue for focusing human resources, facilities, new technology and equipment necessary for the in vivo study of humans and animals. The core serves to fully integrate investigations at the molecular, cellular, and organ system levels that are currently being performed within other cores of the Center and enhance rapid translation of basic discoveries into clinical application. The major services provided by the In Vivo Animal and Human Studies core are as follows:1. To provide a variety of acute and chronic animal models as well as state-of-the-art in vivo technologies to enhance rapid translation of basic discoveries to physiological and clinical applications; 2. To develop an organoid and enteroid program in which these preparations grown from mucosa biopsies and induced pluripotent stem cells (iPSCs) may be used to study epithelial cell biology and provide therapeutic targets for clinical intervention; 3. To support a clinical study program which will provide consultation and technical support to Center investigators on design and statistical data analysis; and 4. To provide a user friendly, one-step service to access digestive disease-related biospecimens for Center members. Since its inception in 1984, the In Vivo Animal and Human Studies core has evolved to meet the needs of Center investigators. Over time, older techniques which are readily available in clinical facilities have been discontinued by the Center. We have streamlined our animal studies to include ones that are frequently utilized by Center investigators. These include mice endoscopy, in vivo cellular and molecular imaging studies in small animals, fluorescent-activated cell sorting (FACS) analysis, and electroporation for direct transfer of siRNA into targeted neuro-tissue or GI organs. The recent development of the Enteroid and Organoid core enables Center investigators to conduct research on in vitro models of human iPSCs, directed differentiation of human pluripotent stem cells (hPSCs), and 3-dimensional growth of primary human and mouse tissue delivered from the GI tract. These preparations may be used to study human GI development, homeostasis and disease in vitro. To enhance the rapid translation of basic science findings to clinical applications, the In Vivo Studies core has spent much of its efforts and resources to upgrade its infrastructure. The Clinical Design Program provides consultation regarding clinical study design, subject specification and sampling techniques, measurements and instruments, sample size estimation, power analysis, as well as data management and statistical analysis. The Biospecimen Banking service will provide support to enhance existing individual GI- related biobanks and develop future repositories. This service also centralizes, expedites, and facilitates access to GI-related biobanks and utilization of GI biospecimens to translate basic research within the paradigm of human specimens. During this funding period, the In Vivo Animal and Human Studies core has been used by 60% of its Center members, has supported 166 publications, with 55 being collaborative.

体内动物和人类研究核心的目标是提供一个集中人类研究的场所 人类和动物体内研究所需的资源、设施、新技术和设备。 其核心是充分整合分子、细胞和器官系统水平的研究 目前正在该中心的其他核心内进行，并加强基本发现的快速转化 进入临床应用。体内动物和人类研究核心提供的主要服务有： 如下： 1．提供各种急性和慢性动物模型以及最先进的体内模型 促进基本发现快速转化为生理学和临床应用的技术； 2. 到 开发类器官和类肠程序，其中这些制剂是从粘膜活检中生长出来的 诱导多能干细胞（iPSC）可用于研究上皮细胞生物学并提供治疗 临床干预的目标； 3. 支持临床研究计划，提供咨询和 为中心研究人员提供设计和统计数据分析方面的技术支持； 4. 提供用户 为中心会员提供友好的一站式服务，获取消化系统疾病相关的生物样本。由于其 体内动物和人类研究核心成立于 1984 年，不断发展以满足中心的需求 调查人员。随着时间的推移，临床设施中易于使用的旧技术已被淘汰。 被中心终止。我们简化了动物研究，将经常出现的动物研究纳入其中 由中心调查员使用。其中包括小鼠内窥镜检查、体内细胞和分子成像研究 在小动物中，荧光激活细胞分选（FACS）分析和电穿孔直接转移 siRNA 进入目标神经组织或胃肠道器官。 Enteroid 和 Organoid 核心的最新进展 使中心研究人员能够对人类 iPSC 的体外模型进行研究，定向分化 人类多能干细胞 (hPSC)，以及原代人类和小鼠组织的 3 维生长 从胃肠道输送。这些制剂可用于研究人类胃肠道发育、体内平衡 和体外疾病。为了促进基础科学发现向临床应用的快速转化，In Vivo Studies core花费了大量的精力和资源来升级其基础设施。临床设计 计划提供有关临床研究设计、主题规范和采样技术的咨询， 测量和仪器、样本量估计、功率分析以及数据管理和 统计分析。生物样本银行服务将为增强现有的个人 GI 提供支持 相关生物库并开发未来的存储库。这项服务还集中、加速和促进 获取与胃肠道相关的生物库并利用胃肠道生物样本来转化该领域内的基础研究 人类标本的范例。在此资助期间，体内动物和人类研究核心已 被 60% 的中心成员使用，已支持 166 种出版物，其中 55 种是协作出版物。