Hybrid Rat Diversity Program

杂交大鼠多样性计划

基本信息

批准号：
9923779
负责人：
MELINDA R DWINELL
金额：
$ 76.95万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2022-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9923779
关键词：
Aging Alcohols Animal Model Animal Sources Animals Anxiety Arthritis Asthma Biological Breeding Characteristics Colorado Communities Complex Cryopreservation Czech Republic DNA Sequence Data Data Analyses Diabetes Mellitus Disease Disease susceptibility Drug usage Elements Embryo Ensure Environment Environmental Impact Environmental Risk Factor Europe Exercise Exposure to Female Future Genes Genetic Genetic Heterogeneity Genetic Models Genetic Polymorphism Genetic Predisposition to Disease Genetic Transcription Genetic Variation Genetic study Genome Genomics Goals Grant Human Hybrids Hypertension Inbred Strain Inbred Strains Rats Inbreeding Infrastructure International Japan Knowledge Laboratory Research Link Location Maintenance Malignant Neoplasms Maps Missouri Modeling Molecular Monoclonal Antibody R24 Nature Pharmacogenomics Phenotype Physiological Positioning Attribute Rat Genome Database Rattus Recombinant Inbred Strain Recombinants Recovery Reproducibility Research Research Personnel Resources Resuscitation Rodent Model Sample Size Site Source Stable Populations Stroke Study models System Techniques Technology Therapeutic Time Tissues Translations Treatment Efficacy Universities Variant Wisconsin animal resource behavioral phenotyping biobank catalyst clinical translation clinically relevant cost data reuse disease mechanisms study drug development drug discovery embryo cryopreservation experimental study genetic resource genome browser genome sequencing genomic data genomic locus male medical schools molecular phenotype mortality phenotypic data potential biomarker precision medicine preference programs reproductive therapeutic target tool trait transcriptome transcriptomics translational medicine whole genome

项目摘要

The overall goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, this project will develop the Hybrid Rat Diversity Panel (HRDP) as the source of the animal models and baseline molecular and physiological phenotypes underlying the National Rat Genetics Resource Program. To achieve this goal, we propose the following four specific aims: 1) to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FEXL/LXFE recombinant inbred strains, 2) to phenotypically characterize, maintain and distribute the HRDP, 3) to complete genomic sequencing on the full HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). This resource will be used to study mechanisms of complex disease traits through systems genetics by providing access to a renewable panel of inbred rat strains and biobanked tissues, reusable phenotypic data, reusable genomic sequence and strain specific variants, and the infrastructure to explore, analyze and visualize these resources through the HRDP Portal at RGD. The HRDP will have broad utility to the scientific community through the genetic and phenotypic diversity represented in the 96 strain panel used to study hypertension, stroke, diabetes, cancer, arthritis, reproductive disorders, asthma, aging, alcohol preference, anxiety, and exercise.

该程序的总体目标是扩展使用大鼠模型的临床翻译的使用建立动物资源和基因组数据集，以促进实验室的翻译研究临床相关性。具体而言，该项目将发展混合大鼠多样性面板（HRDP）作为动物模型的来源以及基线分子和生理国家大鼠遗传学资源计划的基础表型。为了实现这一目标，我们提出以下四个特定目的：1）建立96应变混合大鼠多样性面板由33种经典近交菌株，30 hxb/bxh重组菌株和33个组成 fexl/lxfe重组近交菌株，2）表型表征，维护和分发HRDP，3）在完整的HRDP上完成基因组测序，并提供4）数据分析，集成和传播主要通过大鼠基因组数据库（RGD）。该资源将用于研究复杂疾病特征的机制系统遗传学通过提供对近交大鼠菌株的可再生面板的访问和生物群组织，可重复使用的表型数据，可重复使用的基因组序列和应变特异性变体，以及通过HRDP门户网站探索，分析和可视化这些资源的基础架构 RGD。 HRDP将通过遗传和在研究高血压，中风，糖尿病，癌症，关节炎，生殖疾病，哮喘，衰老，酒精偏爱，焦虑，焦虑，和运动。