Hybrid Rat Diversity Program

杂交大鼠多样性计划

基本信息

批准号：
10411190
负责人：
MELINDA R DWINELL
金额：
$ 77.94万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2026-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10411190
关键词：
Age Aging Alcohols Animal Model Animals Anxiety Arthritis Asthma Atlases Biological Breeding Colorado Communities Complex Controlled Environment Cost of Illness Cryopreservation Czech Republic DNA Sequence Data Data Analyses Development Diabetes Mellitus Discipline Disease Disease Progression Disease susceptibility Embryo Ensure Environment Environmental Impact Environmental Risk Factor Exercise Exposure to Female Foundations Funding Future Gene Targeting Genetic Genetic Heterogeneity Genetic Polymorphism Genetic Predisposition to Disease Genetic Transcription Genetic Variation Genetic study Genome Genomics Goals Grant Human Hybrids Hypertension Inbred Strain Inbred Strains Rats Inbreeding Infrastructure Japan Kidney Knowledge Laboratory Research Location Malignant Neoplasms Maps Missouri Modeling Molecular Monoclonal Antibody R24 Muscle Pharmaceutical Preparations Pharmacogenomics Phenotype Physiological Population Heterogeneity Positioning Attribute Proteome Publications Rat Genome Database Rattus Recombinant Inbred Strain Recombinants Recovery Reproducibility Research Research Personnel Resources Resuscitation Sample Size Sex Differences Site-Directed Mutagenesis Source Stable Populations Stroke System Therapeutic Thymus Gland Tissues Translating Translations Universities Update Variant Visualization software Wisconsin addiction animal resource biobank catalyst clinical translation clinically relevant cost data infrastructure data reuse disease mechanisms study disorder risk drug development embryo cryopreservation epigenome forward genetics genome browser genome sequencing genomic data genomic locus interest male medical schools metabolome molecular phenotype outreach phenotypic data potential biomarker precision medicine preference programs public repository reproductive system disorder stressor symposium therapeutic target tool trait transcriptome transcriptomics webinar whole genome

项目摘要

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. To achieve this goal, we propose the following four specific aims: 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). This resource will be used to study mechanisms of complex disease traits through systems genetics by providing investigators with access to a renewable panel of inbred rat strains and biobanked tissues, reusable phenotypic data, reusable genomic and transcriptomic data, and the infrastructure to explore, analyze and visualize these resources through the HRDP Portal at RGD. Experimental rats and biobanked tissues from the entire HRDP panel can be used for other omic studies, including the epigenome, proteome, and metabolome. The Rat Resource & Research Center (RRRC) is partnering with the HRDP to provide the rat models to the research community. The HRDP will have broad utility to the scientific community through the genetic and phenotypic diversity represented in the 96 strain panel used to study hypertension, stroke, diabetes, cancer, arthritis, reproductive disorders, asthma, aging, alcohol preference, anxiety, exercise and addiction.

项目摘要该程序的持续目标是扩展使用大鼠模型进行临床翻译通过建立动物资源和基因组数据集，以促进翻译实验室研究临床相关性。具体而言，混合大鼠多样性面板（HRDP）资源为研究社区提供了系统遗传学的基础，并为正向遗传学。为了实现这一目标，我们提出以下四个特定目标：1）继续建立由33个经典近交组成的96株混合大鼠多样性面板菌株，30 HXB/BXH重组近交菌株和33个FXLE/LEXF重组植物通过胚胎重新培养和进口，2）表征，维护和分发 HRDP，3）提供HRDP的基因组和转录组测序，并提供4）数据分析，集成和传播主要通过大鼠基因组数据库（RGD）。该资源将用于研究复杂疾病特征的机制系统遗传学通过为研究人员提供可再生大鼠的可再生面板菌株和生物群体组织，可重复使用的表型数据，可重复使用的基因组和转录组学数据以及通过HRDP探索，分析和可视化这些资源的基础架构 RGD的门户网站。来自整个HRDP面板的实验大鼠和生物群体可以是用于其他OMIC研究，包括表观基因组，蛋白质组和代谢组。大鼠资源与研究中心（RRRC）正在与HRDP合作，为老鼠模型提供研究界。 HRDP将通过用于研究的96个应变面板中表示的遗传和表型多样性高血压，中风，糖尿病，癌症，关节炎，生殖疾病，哮喘，衰老，酒精偏爱，焦虑，运动和成瘾。