UCSF DVS CyTOF Mass Cytometer

UCSF DVS CyTOF 质谱流式细胞仪

基本信息

批准号：
8639996
负责人：
LEWIS Lee LANIER
金额：
$ 60万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2016-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The University of California, San Francisco (UCSF) is requesting funds for the purchase of a CyTOF C5 Mass Cytometer by DVS Sciences with Cytobank advanced data management and analytical software. Mass cytometry is a transformational new technology unavailable at UCSF that permits the identification and investigation of rare, diseased cells. The CyTOF mass cytometer marries the resolution, specificity, and sensitivity of atomic mass spectrometry to the high-throughput, single-cell analytical advantages currently provided by flow cytometry. Using stable isotopically pure (non-radioactive) metal tags, as many as forty parameters per single cell can be identified and quantified simultaneously, yielding unique biomarker signatures that distinguish a more refined analysis of cell populations. DVS Sciences is the only mass cytometer manufacturer. The impetus for this application is UCSF's missing mass cytometer capability. There are 43 scientific projects by 21 investigators from 12 different UCSF departments providing justification for funding. Acquisition of the CyTOF mass cytometer will create a shared resource with a projected usage over 2500 hours per year, demonstrating a real need for this technology. Four investigators are currently using the technology through inefficient long-distance collaborations. Clinical biopsies and human samples represent over 80% of the estimated usage of this system, potential biohazards not easily accommodated at other institutions. In response to this technology need, UCSF in a $2M renovation project is building a designated BSL2 (biosafety level) room for a mass cytometry resource. This resource will be part of an existing core facility and includes the ability to construct novel metal-tagged probes, manage CyTOF instrument operation, employ sophisticated software tools for managing and analyzing multi-parametric mass cytometer data, offer continued training and support to the user base, and educational courses to UCSF students. Institutional support will total 17% of the final cost for the CyTOF M5 Mass Cytometer.

描述（由申请人提供）：加州大学旧金山分校 (UCSF) 正在申请资金购买 DVS Sciences 的 CyTOF C5 质谱细胞仪，并配备 Cytobank 先进的数据管理和分析软件。质谱流式细胞术是加州大学旧金山分校不具备的一项变革性新技术，可以识别和研究罕见的患病细胞。 CyTOF 质谱流式细胞仪将原子质谱分析的分辨率、特异性和灵敏度与流式细胞术目前提供的高通量、单细胞分析优势结合起来。使用稳定的同位素纯（非放射性）金属标签，可以同时识别和量化每个单细胞多达四十个参数，产生独特的生物标志物特征，可以对细胞群进行更精细的分析。 DVS Sciences 是唯一的质谱流式细胞仪制造商。该应用的推动力是加州大学旧金山分校缺少的质谱流式细胞仪功能。来自加州大学旧金山分校 12 个不同部门的 21 名研究人员提出了 43 个科学项目，为资助提供了理由。收购 CyTOF 质谱流式细胞仪将创建一个共享资源，预计每年使用时间超过 2500 小时，这表明了对该技术的真正需求。目前，四名研究人员正在通过低效的远程协作来使用该技术。临床活检和人体样本占该系统估计使用量的 80% 以上，其他机构不易应对潜在的生物危害。为了满足这一技术需求，加州大学旧金山分校 (UCSF) 投入了 200 万美元的改造项目，正在为质谱流式细胞术资源建造一个指定的 BSL2（生物安全级别）室。该资源将成为现有核心设施的一部分，包括构建新型金属标记探针、管理 CyTOF 仪器操作、采用复杂的软件工具来管理和分析多参数质谱流式细胞仪数据、为用户提供持续培训和支持的能力为 UCSF 学生提供基础和教育课程。机构支持将占 CyTOF M5 质谱细胞仪最终成本的 17%。