Analyses of sodium bioenergetics in Vibrio cholerae

霍乱弧菌钠生物能学分析

基本信息

批准号：
7192413
负责人：
Claudia C Hase
金额：
$ 33.9万
依托单位：
OREGON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-03-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7192413
关键词：
ATP Synthesis Pathway Affect Animal Model Bacteria Bacterial Physiology Bioenergetics Biological Blood Circulation Cell Survival Cell physiology Cells Characteristics Chromosomes Classification Collection Communicable Diseases Complement Complex Coupling Data Development Devices Ecology Environment Enzymes Flagella Future Gene Expression Regulation Generations Genes Genetic Genome Goals Growth Homeostasis Human Individual Infection Investigation Ion Pumps Ions Lead Life Life Cycle Stages Measurement Membrane Microbe Molecular Molecular Profiling Na(+)-K(+)-Exchanging ATPase Numbers Organism Outcome Pathogenesis Pathway interactions Phase Phenotype Physiology Plasmids Play Property Protein Overexpression Proteins Proton-Motive Force Pump Records Regulation Research Research Personnel Resistance Role Rotation Sodium Solid System Testing Therapeutic Intervention Vibrio cholerae Virulence Virulence Factors Virulent Work antimicrobial drug base concept defined contribution genetic manipulation genome sequencing innovation insight microbial microorganism mutant novel pH Homeostasis pathogen pathogenic bacteria programs research study response sodium ion solute uptake

项目摘要

DESCRIPTION (provided by applicant): All living cells establish transmembrane electrochemical gradients with the help of primary ion pumps. Primary Na+ pumps have been discovered in many microorganisms of quite diverse phylogenic groups and a transmembrane circulation of Na+ ions may play a significant role in the physiology of several bacteria. The recent completion of many bacterial genome sequences revealed the presence of genes encoding a variety of sodium-dependent systems in many organisms, including some that were not known to have a primary sodium cycle of energy. This indicates that these bacteria can utilize Na+ as a coupling ion instead of, or in addition to, the H+ cycle. Although little is known about the role of the sodium cycle of energy in the physiology of Vibrio cholerae, the presence of a multitude of sodium-dependent systems encoded in the genome of this organism merits the investigation of its various Na+ pumps. In the present application we will construct and analyze defined mutants in Na+-extruding enzymes in V. cholerae as a model organism to gain further insights into this very complex and important part of bacterial physiology. We propose a comprehensive and systematic analysis of the components that contribute to bacterial sodium bioenergetics by using extensive genetic manipulations in combination with sophisticated bioenergetic measurements. Results of the proposed study will not only enhance our understanding of the general bioenergetic pathways in bacteria, but will form a solid basis for future investigations, by ourselves and others, of the molecular devices maintaining ion homeostasis in microorganisms. Sodium bioenergetics probably plays a role in both the environmental and pathogenic phases of the V. cholerae life cycle. Thus, our research has the potential for generating fundamental perspectives related to bacterial physiology and will be of value to understanding basic biological concepts related to V. cholerae ecology in the environment and in the lumen of hosts that will be applicable to a variety of bacterial species.

描述（由申请人提供）：所有活细胞在初级离子泵的帮助下建立跨膜电化学梯度。在许多不同系统发育群的微生物中都发现了初级Na+泵，Na+离子的跨膜循环可能在几种细菌的生理学中发挥重要作用。最近完成的许多细菌基因组序列揭示了许多生物体中存在编码多种钠依赖性系统的基因，其中包括一些尚不知道具有初级钠能量循环的生物体。这表明这些细菌可以利用 Na+ 作为偶联离子来代替 H+ 循环，或者作为 H+ 循环的补充。尽管人们对钠能量循环在霍乱弧菌生理学中的作用知之甚少，但该生物体基因组中编码的多种钠依赖性系统的存在值得对其各种钠泵进行研究。在本申请中，我们将构建并分析霍乱弧菌中 Na+ 挤出酶的确定突变体作为模式生物，以进一步了解细菌生理学中这一非常复杂且重要的部分。我们建议通过使用广泛的遗传操作与复杂的生物能测量相结合，对有助于细菌钠生物能的成分进行全面和系统的分析。拟议研究的结果不仅将增强我们对细菌中一般生物能途径的理解，而且将为我们自己和其他人未来研究维持微生物中离子稳态的分子装置奠定坚实的基础。钠生物能学可能在霍乱弧菌生命周期的环境阶段和致病阶段都发挥作用。因此，我们的研究有可能产生与细菌生理学相关的基本观点，并且对于理解与环境和宿主内腔中的霍乱弧菌生态学相关的基本生物学概念具有价值，这些概念将适用于多种细菌物种。