Analyses of sodium bioenergetics in Vibrio cholerae

霍乱弧菌钠生物能学分析

基本信息

批准号：
7367043
负责人：
Claudia C Hase
金额：
$ 33.22万
依托单位：
OREGON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-03-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7367043
关键词：
ATP Synthesis Pathway Affect Animal Model Bacteria Bacterial Physiology Bioenergetics Biological Blood Circulation Cell Survival Cell physiology Cells Characteristics Chromosomes Classification Collection Communicable Diseases Complement Complex Coupling Data Development Devices Ecology Environment Enzymes Flagella Future Gene Expression Regulation Generations Genes Genetic Genome Goals Growth Homeostasis Human Individual Infection Investigation Ion Pumps Ions Lead Life Life Cycle Stages Measurement Membrane Microbe Molecular Molecular Profiling Na(+)-K(+)-Exchanging ATPase Numbers Organism Outcome Pathogenesis Pathway interactions Phase Phenotype Physiology Plasmids Play Property Protein Overexpression Proteins Proton-Motive Force Pump Records Regulation Research Research Personnel Resistance Role Rotation Sodium Solid System Testing Therapeutic Intervention Vibrio cholerae Virulence Virulence Factors Virulent Work antimicrobial drug base concept defined contribution genetic manipulation genome sequencing innovation insight microbial microorganism mutant novel pH Homeostasis pathogen pathogenic bacteria programs research study response sodium ion solute uptake

项目摘要

All living cells establish transmembrane electrochemical gradients with the help of primary ion pumps. Primary Na+ pumps have been discovered in many microorganisms of quite diverse phylogenic groups and a transmembrane circulation of Na+ ions may play a significant role in the physiology of several bacteria. The recent completion of many bacterial genome sequences revealed the presence of genes encoding a variety of sodium-dependent systems in many organisms, including some that were not known to have a primary sodium cycle of energy. This indicates that these bacteria can utilize Na+ as a coupling ion instead of, or in addition to, the H+ cycle. Although little is known about the role of the sodium cycle of energy in the physiology of Vibrio cholerae, the presence of a multitude of sodium-dependent systems encoded in the genome of this organism merits the investigation of its various Na+ pumps. In the present application we will construct and analyze defined mutants in Na+-extruding enzymes in V. cholerae as a model organism to gain further insights into this very complex and important part of bacterial physiology. We propose a comprehensive and systematic analysis of the components that contribute to bacterial sodium bioenergetics by using extensive genetic manipulations in combination with sophisticated bioenergetic measurements. Results of the proposed study will not only enhance our understanding of the general bioenergetic pathways in bacteria, but will form a solid basis for future investigations, by ourselves and others, of the molecular devices maintaining ion homeostasis in microorganisms. Sodium bioenergetics probably plays a role in both the environmental and pathogenic phases of the V. cholerae life cycle. Thus, our research has the potential for generating fundamental perspectives related to bacterial physiology and will be of value to understanding basic biological concepts related to V. cholerae ecology in the environment and in the lumen of hosts that will be applicable to a variety of bacterial species.

所有活细胞都在初级离子泵的帮助下建立跨膜电化学梯度。初级Na+泵已在许多不同系统发育群体的微生物中被发现，并且 Na+离子的跨膜循环可能在几种细菌的生理学中发挥重要作用。最近完成的许多细菌基因组序列揭示了编码a的基因的存在许多生物体中存在多种钠依赖性系统，包括一些未知的钠依赖性系统初级钠能量循环。这表明这些细菌可以利用Na+作为偶联离子 H+循环的或补充。尽管人们对钠能量循环在能量循环中的作用知之甚少霍乱弧菌的生理学，编码的多种钠依赖性系统的存在该生物体的基因组值得对其各种 Na+ 泵进行研究。在本申请中，我们将构建并分析霍乱弧菌中 Na+ 挤出酶的确定突变体作为模型生物进一步了解细菌生理学这一非常复杂且重要的部分。我们提出一个全面系统地分析有助于细菌钠生物能的成分通过使用广泛的基因操作结合复杂的生物能测量。拟议研究的结果不仅将增强我们对一般生物能量途径的理解在细菌中，但将为我们自己和其他人未来的分子研究奠定坚实的基础维持微生物离子稳态的装置。钠生物能学可能在以下两个方面都发挥作用霍乱弧菌生命周期的环境和致病阶段。因此，我们的研究有潜力产生与细菌生理学相关的基本观点，并且对于理解具有价值与环境和宿主内腔中霍乱弧菌生态相关的基本生物学概念适用于多种细菌种类。