Analyses of sodium bioenergetics in Vibrio cholerae

霍乱弧菌钠生物能学分析

• 批准号: 7103161
• 负责人: Claudia C Hase
• 金额: $ 34.95万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7103161
• 关键词: Vibrio cholerae; bacteria; bioenergetics; genes; physiology; role; sodium; virulence; Vibrio cholerae; bacteria; bioenergetics; genes; physiology; role; sodium; virulence

DESCRIPTION (provided by applicant): All living cells establish transmembrane electrochemical gradients with the help of primary ion pumps. Primary Na+ pumps have been discovered in many microorganisms of quite diverse phylogenic groups and a transmembrane circulation of Na+ ions may play a significant role in the physiology of several bacteria. The recent completion of many bacterial genome sequences revealed the presence of genes encoding a variety of sodium-dependent systems in many organisms, including some that were not known to have a primary sodium cycle of energy. This indicates that these bacteria can utilize Na+ as a coupling ion instead of, or in addition to, the H+ cycle. Although little is known about the role of the sodium cycle of energy in the physiology of Vibrio cholerae, the presence of a multitude of sodium-dependent systems encoded in the genome of this organism merits the investigation of its various Na+ pumps. In the present application we will construct and analyze defined mutants in Na+-extruding enzymes in V. cholerae as a model organism to gain further insights into this very complex and important part of bacterial physiology. We propose a comprehensive and systematic analysis of the components that contribute to bacterial sodium bioenergetics by using extensive genetic manipulations in combination with sophisticated bioenergetic measurements. Results of the proposed study will not only enhance our understanding of the general bioenergetic pathways in bacteria, but will form a solid basis for future investigations, by ourselves and others, of the molecular devices maintaining ion homeostasis in microorganisms. Sodium bioenergetics probably plays a role in both the environmental and pathogenic phases of the V. cholerae life cycle. Thus, our research has the potential for generating fundamental perspectives related to bacterial physiology and will be of value to understanding basic biological concepts related to V. cholerae ecology in the environment and in the lumen of hosts that will be applicable to a variety of bacterial species.

描述（由申请人提供）：所有活细胞在原代离子泵的帮助下建立了跨膜电化学梯度。在许多多样化的系统成立基团的微生物中发现了原发性Na+泵，Na+离子的跨膜循环可能在几种细菌的生理学中起重要作用。最近完成许多细菌基因组序列的完成揭示了许多生物体中编码各种钠依赖性系统的基因，其中包括一些未知的基因，这些系统具有原发性的能量钠周期。这表明这些细菌可以利用Na+作为耦合离子，而不是H+循环。尽管对能量钠周期在弧菌霍乱生理学中的作用知之甚少，但该生物基因组中编码的多种钠依赖性系统的存在值得研究其各种Na+泵。在本应用中，我们将构建和分析霍乱弧菌中Na+侵犯酶的定义突变体，作为模型生物体，以进一步了解细菌生理的这一非常复杂和重要的部分。我们通过使用广泛的遗传操作与复杂的生物能测量方法结合使用广泛的遗传操作来对成分进行全面的系统分析。拟议的研究的结果不仅将增强我们对细菌中一般生物能途径的理解，而且还将为我们自己和其他人，维持微生物中离子稳态的分子设备的未来研究构成可靠的基础。生物能钠可能在V.霍乱生命周期的环境和致病阶段起作用。因此，我们的研究具有产生与细菌生理学相关的基本观点的潜力，并将具有重要的价值，以了解与环境和宿主的腔内相关的基本生物学概念，这些概念适用于各种细菌物种。