Mechanism and clinical pilot tst of volar fibroblast to create ectopic volar skin

掌侧成纤维细胞形成异位掌侧皮肤的机制及临床试验

• 批准号: 9322010
• 负责人: Luis Andres Garza
• 金额: $ 35.82万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9322010
• 关键词: 3-Dimensional; Abdomen; Adult; Amputees; Animal Model; Area; Autologous; Back; Biopsy; Buttocks; Cells; Cheek structure; Clinical; Coculture Techniques; Data; Dermatologic; Dermatology; Dose; Down-Regulation; Ear; Epidermis; Fibroblasts; Frequencies; Friction; Future; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Transcription; Goals; Grant; Hand; Histology; Homeobox Genes; Human; In Vitro; Injectable; Investigational New Drug Application; Irritants; Keratin; Learning; Lip structure; Location; Longevity; Maps; Medicine; Messenger RNA; Minority; Monitor; Overlapping Genes; Pain; Phenotype; Process; Prosthesis; Recombinant Proteins; Regenerative Medicine; Research; Resistance; Reverse Transcriptase Polymerase Chain Reaction; Series; Site; Skin; Small Interfering RNA; Stratum corneum; Testing; Thick; Tissues; Transfection; Up-Regulation; experimental study; foot; human subject; in vivo; keratinocyte; knock-down; limb amputation; mutant; neutralizing antibody; non-invasive imaging; penis foreskin; public health relevance; transcription factor; volunteer

DESCRIPTION (provided by applicant): The long term objective of the proposed research is to learn how to effectively use cellular therapy in regenerative medicine, especially as pertains to dermatology. In particular, our goal is to convert the identity of skin at the stump site of amputees to thick palmo/plantar (volar) type skin to enhance their use of prosthetics. Towards this clinical goal we first will define the basic genetic control of volar fibroblast skin identity Then we will define homeostatic mechanisms whereby non-volar tissue retains its identity. In so doing, we will optimize conditions for our final effort to use autologous fibroblasts to induce ectopic volar skin in volunteers. We will fully excise this ectopic acral skin as a proof-of-concep trial and a prelude to clinical optimization and use in amputees.

描述（由申请人提供）：拟议研究的长期目标是了解如何在再生医学中有效地使用细胞疗法，特别是在皮肤病学方面。特别是，我们的目标是将截肢者残肢部位的皮肤转变为厚的掌/足底（掌）型皮肤，以增强他们对假肢的使用。为了实现这一临床目标，我们首先将定义掌侧成纤维细胞皮肤特性的基本遗传控制，然后我们将定义非掌侧组织保持其特性的稳态机制。在此过程中，我们将优化最终努力的条件，以使用自体成纤维细胞在志愿者中诱导异位掌侧皮肤。我们将完全切除这种异位肢端皮肤，作为概念验证试验以及临床优化和在截肢者中使用的前奏。

项目成果

Does prostaglandin D2 hold the cure to male pattern baldness?

• DOI: 10.1111/exd.12348
• 发表时间: 2014-04
• 期刊: Experimental dermatology
• 影响因子: 3.6
• 作者: Nieves A;Garza LA
• 通讯作者: Garza LA

Improving acne keloidalis nuchae with targeted ultraviolet B treatment: a prospective, randomized, split-scalp comparison study.

• DOI: 10.1111/bjd.13119
• 发表时间: 2014-11
• 期刊: The British journal of dermatology
• 作者: Okoye GA;Rainer BM;Leung SG;Suh HS;Kim JH;Nelson AM;Garza LA;Chien AL;Kang S
• 通讯作者: Kang S

Hairy math: addition of Wnt-3a to multiply bulge cells.

毛茸茸的数学：添加 Wnt-3a 来繁殖凸出细胞。

• DOI: 10.1038/jid.2014.545
• 发表时间: 2015
• 期刊: The Journal of investigative dermatology
• 作者: ZulfiquerHossain,M;Garza,LuisA
• 通讯作者: Garza,LuisA

After Skin Wounding, Noncoding dsRNA Coordinates Prostaglandins and Wnts to Promote Regeneration.

• DOI: 10.1016/j.jid.2017.03.023
• 发表时间: 2017-07
• 期刊: The Journal of investigative dermatology
• 作者: Zhu AS;Li A;Ratliff TS;Melsom M;Garza LA
• 通讯作者: Garza LA

Efficacy and Tolerability of a Novel Biopsy Device for Removing Benign Epidermal Skin Lesions.

用于去除良性表皮皮肤病变的新型活检装置的功效和耐受性。

• DOI: 10.1097/dss.0000000000000530
• 发表时间: 2015
• 期刊: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]
• 作者: Yang,StevenHoseong;Qi,Ji;Esandrio,Jessica;Leung,Sherry;Taube,JanisM;Garza,LuisA;Allen,Robert;Kang,Sewon;Chien,AnnaL
• 通讯作者: Chien,AnnaL