Retinoic acid synthesis induced by noncoding dsRNA controls Regeneration

非编码 dsRNA 诱导的视黄酸合成控制再生

• 批准号: 10684735
• 负责人: Luis Andres Garza
• 金额: $ 46.46万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-25 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10684735
• 关键词: Affect; Apocrine Glands; Biological Models; Biology; Blood Vessels; Chemicals; Cicatrix; Combined Modality Therapy; Data; Dermatology; Development; Diagnostic; Double-Stranded RNA; Eccrine Glands; Embryonic Development; Enzymes; Excision; Fascia; Feedback; Fibrosis; Frequencies; Future; Gene Expression; Genes; Genetic Transcription; Geography; Goals; Grant; Hair; Hair follicle structure; Health; Heart; Homeostasis; Human; Injury; Knockout Mice; Lasers; Learning; Ligands; Location; Lung; Mass Spectrum Analysis; Molecular Chaperones; Mus; Myocardial Infarction; Natural regeneration; Neomycin resistance gene; Nerve; Pathology; Pathway interactions; Production; Proteomics; Pulmonary Fibrosis; Recurrence; Research; Retina; Retinoic Acid Receptor; Role; Signal Induction; Signal Transduction; Skin; Skin injury; Spottings; Structure; TLR3 gene; Testing; Therapeutic; Tissues; Topical application; Translating; Tretinoin; Untranslated RNA; Wild Type Mouse; appendage; cell type; chronic wound; hair regeneration; healing; heart function; human subject; in vivo; inhibitor; injured; insight; keratinocyte; novel therapeutics; prevent; protein expression; receptor; response; retinoic acid receptor alpha; skin regeneration; skin wound; synergism; transcriptomics; wound; wound healing

Project Summary Abstract The long term objective of the proposed research is to learn what controls whether injuries heal with scarring/fibrosis versus full regeneration of lost structures. The human health burden of fibrosis and scarring is enormous and affects every tissue from heart (lost heart function after a myocardial infarction) to lung (idiopathic lung fibrosis). Enhancing regeneration could prevent for example the almost universal recurrence of chronic wounds in the exact location where they previously appeared. We use the Wound Induced Hair Neogenesis (WIHN) model system where in the center of excisional wounds in mice a variable amount of regeneration occurs and de novo hair follicles form in a recapitulation of embryogenesis. The goal of this grant is to understand the factors which control the frequency of regeneration. We will directly test the mechanism and the ability for candidate molecules which enhance regeneration in mice and in human subjects. The results of this grant promise to help define new treatments and diagnostics to enhance regeneration and wound healing.

项目概要 摘要 拟议研究的长期目标是了解是什么控制着损伤是否会随着时间的推移而愈合 疤痕/纤维化与丢失结构的完全再生。纤维化和疤痕对人类健康造成的负担 巨大，影响从心脏（心肌梗死后失去心脏功能）到肺的每个组织 （特发性肺纤维化）。增强再生可以防止几乎普遍的复发 慢性伤口位于先前出现的确切位置。我们使用伤口诱导头发 新生（WIHN）模型系统，其中在小鼠切除伤口的中心有不同数量的 再生发生并从头形成毛囊，重现胚胎发生过程。本次赠款的目标 就是要了解控制再生频率的因素。我们将直接测试该机制 以及候选分子增强小鼠和人类受试者再生的能力。结果 这笔赠款的一部分承诺帮助确定新的治疗和诊断方法，以增强再生和伤口 康复。

项目成果

Clinical and molecular change induced by repeated low-dose visible light exposure in both light-skinned and dark-skinned individuals.

浅色皮肤和深色皮肤个体重复低剂量可见光照射引起的临床和分子变化。

• DOI: 10.1111/phpp.12819
• 发表时间: 2023
• 期刊: Photodermatology, photoimmunology & photomedicine
• 作者: Kim,Sooyoung;Rainer,BarbaraM;Qi,Ji;Brown,Isabelle;Ogurtsova,Aleksandra;Leung,Sherry;Garza,LuisA;Kang,Sewon;Chien,AnnaL
• 通讯作者: Chien,AnnaL

Toward Understanding Wound Immunology for High-Fidelity Skin Regeneration.

了解伤口免疫学以实现高保真皮肤再生。

• DOI: 10.1101/cshperspect.a041241
• 发表时间: 2022
• 期刊: Cold Spring Harbor perspectives in biology
• 影响因子: 7.2
• 作者: Xue,Yingchao;Reddy,SashankK;Garza,LuisA
• 通讯作者: Garza,LuisA

Topical timolol 0.5% gel-forming solution for erythema in rosacea: A quantitative, split-face, randomized, and rater-masked pilot clinical trial.

• DOI: 10.1016/j.jaad.2021.01.098
• 发表时间: 2021-10
• 期刊: Journal of the American Academy of Dermatology
• 影响因子: 13.8
• 作者: Tsai J;Chien AL;Kim N;Rachidi S;Connolly BM;Lim H;Alessi César SS;Kang S;Garza LA
• 通讯作者: Garza LA

Hyperspectral measurement of skin reflectance detects differences in the visible and near-infrared regions according to race, gender and body site.

• DOI: 10.1111/jdv.17076
• 发表时间: 2021-05
• 期刊: Journal of the European Academy of Dermatology and Venereology : JEADV
• 作者: Tsai J;Chien AL;Kang JU;Leung S;Kang S;Garza LA
• 通讯作者: Garza LA

Association of Early Clinical Response to Laser Rejuvenation of Photoaged Skin with Increased Lipid Metabolism and Restoration of Skin Barrier Function.

• DOI: 10.1016/j.jid.2022.07.024
• 发表时间: 2023-03
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Garza, Luis A.;Sheu, Mary;Kim, Noori;Tsai, Jerry;Cesar, Sabrina S. Alessi;Lee, Jianming;Hawkins, Stacy S.;Chien, Anna L.;Kang, Sewon
• 通讯作者: Kang, Sewon