Injection of autologous volar fibroblasts to the stump site to allow pressure adaptation and enhanced prosthetic use in amputees

将自体掌侧成纤维细胞注射到残肢部位，以适应截肢者的压力并增强假肢的使用

• 批准号: 10492492
• 负责人: Luis Andres Garza
• 金额: $ 53.22万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-22 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10492492
• 关键词: Address; Affect; Amputation; Amputees; Autologous; Biological Models; Biology; Biopsy; Categories; Cell Therapy; Cells; Clinical; Clinical Trials; Collagen; Cytoplasm; Data; Dermis; Development; Disease; Dose; Engraftment; Enrollment; Environment; Epithelium; Face; Fibroblasts; Freezing; Funding; Future; Gene Expression; Genes; Genetic Diseases; Goals; Grant; Histologic; Human; Infection; Injections; Intervention; Large Keratinocyte; Lead; Length; Limb structure; Maryland; Medicine; Mesenchyme; Modeling; Molecular; Morbidity - disease rate; Morphogenesis; Mus; Ontology; Pain; Patients; Phase; Population; Production; Property; Prosthesis; Prosthesis Design; Protein Deficiency; Proteins; Protocols documentation; Quality of life; Regenerative Medicine; Research; Schedule; Side; Site; Skin; Testing; Thick; Time; Tissues; Transduction Gene; Viral Vector; Work; decubitus ulcer; enzyme replacement therapy; foot sole; gene correction; gene therapy; healthy volunteer; human subject; human tissue; improved; in vivo; keratinocyte; next generation; novel therapeutics; organ growth; phase II trial; pressure; prevent; regenerative cell; resilience; single-cell RNA sequencing; stem cell therapy; transcriptome sequencing; treatment optimization; trial enrollment; wounded soldier

Project Summary Abstract The long term objective of the proposed research is to use cellular therapy to advance regenerative medicine. In particular, we wish to help amputees who have lost a limb. While major improvements have been made to prosthetics, the skin side of the interface at the stump site was not evolved to bear weight. The stump does not normally have the pressure adaptive abilities of the soles of the foot for example. The present grant proposes to biopsy, purify and expand fibroblasts from an existing palm or sole and inject them into the stump site of an amputee to improve their use of prosthetics. We also propose to study the basic biology behind cell therapy to understand how the injected cells change the native cells, and in turn how those native cells affect the injected cells. The results of this grant will hopefully lead to a new therapy to help not only amputees, but potentially prevent pressure ulcers and provide a platform for future cell therapies.

项目概要 摘要 拟议研究的长期目标是利用细胞疗法来推进再生医学。 我们特别希望帮助失去肢体的截肢者。虽然已经做出了重大改进 在假肢中，残肢部位界面的皮肤侧尚未进化到能够承受重量。树桩不 例如，通常具有脚底的压力适应能力。目前的赠款建议 对现有手掌或脚掌进行活检、纯化和扩增成纤维细胞，并将其注射到残肢部位 截肢者改善假肢的使用。我们还建议研究细胞疗法背后的基础生物学 了解注射的细胞如何改变天然细胞，以及这些天然细胞如何影响注射的细胞 细胞。这笔赠款的结果有望带来一种新的疗法，不仅可以帮助截肢者，而且有可能帮助 预防压疮并为未来的细胞疗法提供平台。