Coordinated regulation of floral transition by protein and long noncoding RNA components

蛋白质和长非编码RNA成分对花转变的协调调控

基本信息

批准号：
9384248
负责人：
Sibum Sung
金额：
$ 30.4万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2021-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9384248
关键词：
Address Angiosperms Animals Arabidopsis Biological Biological Models Biological Process ChIP-seq Chromatin Chromatin Loop Code Complex Cues Data Data Analyses Development Drosophila genus Elements Environment Epigenetic Process Eukaryota Eukaryotic Cell Event Experimental Models Gene Expression Gene Expression Regulation Genes Genetic Transcription Genome Goals Histone H3 Human Immunoprecipitation In Vitro Knowledge Life Cycle Stages Lysine Mass Spectrum Analysis Mediating Methylation Modeling Molecular Molecular Conformation Nature Organism PHD Finger Phenotype Plant Roots Plants Play Polycomb Process Proteins RNA Regulation Regulator Genes Repression Research Role Series Study models System Testing Time Untranslated RNA Variant Work Yeasts chromatin immunoprecipitation chromatin modification chromatin remodeling deep sequencing demethylation design epigenetic regulation experimental study gene repression genetic approach genome-wide histone demethylase histone methyltransferase histone modification in vitro Assay insight plant growth/development predictive modeling programs protein expression response scaffold three dimensional structure transcriptome sequencing

项目摘要

Coordinated regulation of floral transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable organisms to adapt to developmental and environmental cues. Flowering is one of the major developmental commitments in the plant life cycle. Plants have evolved intricate regulatory networks to control flowering time in response to both endogenous and environmental conditions. For example, prolonged cold triggers vernalization, a process that accelerates flowering through epigenetic changes in genes involved in development. Therefore, the vernalization response in Arabidopsis is an excellent model system for study of complex epigenetic regulation of gene expression triggered by environmental cues in eukaryotes. Vernalization-mediated epigenetic changes include the formation of chromatin loops and alterations in chromatin modifications and in expression of long noncoding RNAs (lncRNAs). Changes in three-dimensional structure of genome, such as formation of local chromatin loops, are increasingly recognized as important gene regulatory events in eukaryotes; however, how chromatin modifications and lncRNAs coordinate to influence gene expression through changes in three-dimensional structure of genome is not well understood. Here we seek to understand the mechanistic details of how chromatin modifications and lncRNAs mediate gene regulation. Our overriding goal is to elucidate structural and regulatory components governing protein-lncRNA mediated epigenetic gene regulation during vernalization in Arabidopsis through three specific aims designed to: 1) elucidate the detailed mechanisms of lncRNA-mediated gene regulation focusing on two lncRNAs that we showed coordinate the repression of gene involved flowing through the formation of intragenic chromatin loop, 2) evaluate coordination between protein and lncRNA components that mediate chromatin modification, 3) perform functional characterization of additional loci that undergo epigenetic changes upon developmental transition. Our approach will reveal the mechanistic details of lncRNA-mediated epigenetic regulation as it occurs during a key process of plant development (i.e., flowering). These findings will further our understanding of the mechanism of epigenetic regulation of gene expression, which has a deep evolutionary root among eukaryotes.

通过蛋白质和长未编码RNA成分的花卉过渡的协调调节项目摘要表观遗传机制使生物可以适应发育和环境线索。开花是植物生命周期中的主要发展承诺。植物已经进化了复杂的调节网络以控制响应内源性和环境条件的开花时间。例如，长时间的冷触发器春化，这一过程通过涉及发育的基因的表观遗传变化加速开花。因此，拟南芥中的春化反应是研究复杂表观遗传的出色模型系统由真核生物中环境线索触发的基因表达的调节。春化介导的表观遗传变化包括染色质环的形成和染色质修饰的改变以及长期表达非编码RNA（LNCRNA）。基因组的三维结构的变化，例如局部染色质的形成循环越来越被认为是真核生物中重要的基因调节事件。但是，染色质修饰和LNCRNA坐标以通过变化的三维结构的变化来影响基因表达基因组不太了解。在这里，我们试图了解染色质修饰和如何修饰的机械细节 LNCRNA介导基因调节。我们的压倒性目标是阐明管理的结构和监管组件蛋白质lncRNA介导的表观遗传基因调节在拟南芥中通过三个特定目标设计为：1）阐明LNCRNA介导的基因调节的详细机制，重点是两个lncrnas 我们展示了基因的抑制，涉及通过基因内染色质环的形成流动的抑制作用，2）评估介导染色质修饰的蛋白质和lncRNA成分之间的协调，3）执行在发育过渡时经历表观遗传变化的其他基因座的功能表征。我们的方法将揭示LNCRNA介导的表观遗传调节的机械细节，因为它在关键过程中发生植物发育（即开花）。这些发现将进一步我们对表观遗传机制的理解基因表达的调节，在真核生物之间具有深层进化。