Coordinated regulation of developmental transition by protein and long noncoding RNA components

蛋白质和长链非编码RNA成分对发育转变的协调调控

• 批准号: 10798906
• 负责人: Sibum Sung
• 金额: $ 5.07万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798906
• 关键词: Acceleration; Arabidopsis; Architecture; Biological Models; Chromatin; Chromatin Loop; Chromatin Structure; Complex; Cues; Development; Drosophila genus; Environment; Epigenetic Process; Eukaryota; Event; Flowers; Gene Expression; Gene Expression Regulation; Genes; Genome; Goals; Human; Knowledge; Light; Mediating; Molecular Conformation; Nature; Organism; Plants; Process; Proteins; Regulation; Regulator Genes; Signal Transduction; Stimulus; Study models; Temperature; Untranslated RNA; Yeasts; chromatin modification; design; epigenetic regulation; genome-wide; in vivo; programs; response; three dimensional structure

Coordinated regulation of developmental transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable organisms to adapt to developmental and environmental cues. Plants have evolved intricate regulatory networks to control development in response environmental stimuli such as temperature and light. For example, prolonged cold triggers vernalization, a process that accelerates flowering through epigenetic changes in genes involved in development. Therefore, the vernalization response in Arabidopsis is an excellent model system for study of complex epigenetic regulation of gene expression triggered by environmental cues in eukaryotes. Vernalization-mediated epigenetic changes include the formation of chromatin loops and alterations in chromatin modifications and in expression of long noncoding RNAs (lncRNAs). Changes in the three-dimensional structure of the genome, such as formation of local chromatin loops, are increasingly recognized as important gene regulatory events in eukaryotes; however, how chromatin modifications and lncRNAs coordinate to influence gene expression through changes in the three-dimensional structure of the genome is not well understood. Here we seek to understand the mechanistic details of how the three-dimensional structure of the genome influences gene regulation in vivo using developmental changes in flowering and photomorphogenesis as phenotypical read- outs. Our overriding goal is to elucidate structural and regulatory components governing protein- and lncRNA-mediated epigenetic gene regulation in Arabidopsis through three specific aims designed to: 1) elucidate the detailed mechanisms of chromatin structure-mediated gene regulation focusing on two groups of chromatin architectural proteins that we showed coordinate gene regulation through the formation of chromatin loops, 2) characterize mechanisms underlying light signaling-mediated chromatin conformation changes, and 3) characterize genome-wide changes in chromatin structure induced by environmental stimuli. Our approach will reveal the mechanistic details of chromatin structure-based epigenetic regulation by both protein and lncRNA components during flowering and photomorphogenesis. These findings will further our understanding of the mechanism of epigenetic regulation of gene expression, which has a deep evolutionary root among eukaryotes.

蛋白质和长链非编码RNA成分对发育转变的协调调控 项目概要 表观遗传机制使生物体能够适应发育和环境线索。植物有 进化出复杂的监管网络来控制发展以响应环境刺激，例如 温度和光照。例如，长时间的寒冷会引发春化，这一过程会加速 通过参与发育的基因的表观遗传变化来开花。因此，春化 拟南芥反应是研究基因复杂表观遗传调控的优秀模型系统 真核生物中由环境线索触发的表达。春化介导的表观遗传变化 包括染色质环的形成以及染色质修饰和长链表达的改变 非编码 RNA (lncRNA)。基因组三维结构的变化，例如形成 局部染色质环越来越被认为是真核生物中重要的基因调控事件； 然而，染色质修饰和lncRNA如何协调通过影响基因表达 基因组三维结构的变化尚不清楚。在这里我们寻求 了解基因组三维结构如何影响基因的机制细节 使用开花和光形态发生的发育变化作为表型读取的体内调节 出局。我们的首要目标是阐明控制蛋白质和蛋白质的结构和调控成分 拟南芥中 lncRNA 介导的表观遗传基因调控通过三个具体目标设计：1) 阐明染色质结构介导的基因调控的详细机制，重点关注两组 我们展示了染色质结构蛋白通过形成 染色质环，2) 表征光信号介导的染色质构象的机制 变化，3) 表征环境刺激引起的染色质结构的全基因组变化。 我们的方法将揭示基于染色质结构的表观遗传调控的机制细节 开花和光形态发生过程中的蛋白质和lncRNA成分。这些发现将进一步推动我们 了解基因表达的表观遗传调控机制，具有深刻的进化意义 真核生物中的根。