Coordinated regulation of developmental transition by protein and long noncoding RNA components

蛋白质和长链非编码RNA成分对发育转变的协调调控

基本信息

批准号：
10544333
负责人：
Sibum Sung
金额：
$ 32.11万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10544333
关键词：
26S proteasome 3-Dimensional Acceleration Address Affect Animals Arabidopsis Architecture Biological Biological Models Cell Nucleus Chromatin Chromatin Loop Chromatin Structure Complex Cues Data Development Drosophila genus Environment Epigenetic Process Eukaryota Eukaryotic Cell Event Experimental Models Flowers Gene Expression Gene Expression Regulation Genes Genetic Transcription Genome Goals HMGN Proteins Human Knowledge Light Mediating Modeling Molecular Conformation Nature Nucleosomes Organism Phenotype Photoreceptors Plants Play Polycomb Process Proteins Regulation Regulator Genes Repression Research Role SPT6 Protein Signal Transduction Stimulus Structure Study models System Temperature Testing Ubiquitin Untranslated RNA Work Yeasts bioinformatics pipeline chromatin modification chromatin protein design epigenetic regulation experimental study genetic regulatory protein genome-wide high throughput analysis histone modification in vivo phyB phytochrome predictive modeling programs protein degradation response three dimensional structure

项目摘要

Coordinated regulation of developmental transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable organisms to adapt to developmental and environmental cues. Plants have evolved intricate regulatory networks to control development in response environmental stimuli such as temperature and light. For example, prolonged cold triggers vernalization, a process that accelerates flowering through epigenetic changes in genes involved in development. Therefore, the vernalization response in Arabidopsis is an excellent model system for study of complex epigenetic regulation of gene expression triggered by environmental cues in eukaryotes. Vernalization-mediated epigenetic changes include the formation of chromatin loops and alterations in chromatin modifications and in expression of long noncoding RNAs (lncRNAs). Changes in the three-dimensional structure of the genome, such as formation of local chromatin loops, are increasingly recognized as important gene regulatory events in eukaryotes; however, how chromatin modifications and lncRNAs coordinate to influence gene expression through changes in the three-dimensional structure of the genome is not well understood. Here we seek to understand the mechanistic details of how the three-dimensional structure of the genome influences gene regulation in vivo using developmental changes in flowering and photomorphogenesis as phenotypical read- outs. Our overriding goal is to elucidate structural and regulatory components governing protein- and lncRNA-mediated epigenetic gene regulation in Arabidopsis through three specific aims designed to: 1) elucidate the detailed mechanisms of chromatin structure-mediated gene regulation focusing on two groups of chromatin architectural proteins that we showed coordinate gene regulation through the formation of chromatin loops, 2) characterize mechanisms underlying light signaling-mediated chromatin conformation changes, and 3) characterize genome-wide changes in chromatin structure induced by environmental stimuli. Our approach will reveal the mechanistic details of chromatin structure-based epigenetic regulation by both protein and lncRNA components during flowering and photomorphogenesis. These findings will further our understanding of the mechanism of epigenetic regulation of gene expression, which has a deep evolutionary root among eukaryotes.

通过蛋白质和长不编码RNA成分的协调调节发育过渡项目摘要表观遗传机制使生物可以适应发育和环境线索。植物有进化的复杂的调节网络以控制反应环境刺激的发展，例如温度和光。例如，长时间的冷触发因素，这一过程加速了通过涉及发育的基因的表观遗传变化开花。因此，春化拟南芥中的反应是研究基因复杂表观遗传调节的出色模型系统由真核生物中的环境提示触发的表达。春化介导的表观遗传变化包括染色质环的形成和染色质修饰的改变以及长期表达非编码RNA（LNCRNA）。基因组的三维结构的变化，例如形成局部染色质环越来越被认为是真核生物中重要的基因调节事件。但是，染色质修饰和LNCRNA如何通过基因组的三维结构的变化尚不清楚。我们在这里寻求了解基因组三维结构如何影响基因的机械细节使用开花和光形态发生的发展变化作为表型读取 - 出口。我们的压倒性目标是阐明管理蛋白质和蛋白质和调节的组件 LNCRNA介导的拟南芥中的表观遗传学基因调节通过三个特定目的：1）阐明染色质结构介导的基因调节的详细机制，重点是两组染色质结构蛋白，我们通过形成显示了坐标基因调节染色质环，2）表征光信号传导介导的染色质构象的机制变化和3）表征环境刺激引起的染色质结构的全基因组变化。我们的方法将通过两者都揭示基于染色质结构的表观遗传调节的机械细节在开花和显微形态发生过程中的蛋白质和lncRNA成分。这些发现将进一步了解基因表达的表观遗传调节的机制，该机制具有深层进化真核生物之间的根。