Thalamic reticular nucleus modulation of auditory thalamocortical function

丘脑网状核对听觉丘脑皮质功能的调节

基本信息

批准号：
9096739
负责人：
DANIEL A LLANO
金额：
$ 19.83万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-06-23 至 2018-09-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9096739
关键词：
Acoustics Action Potentials Address Amygdaloid structure Attention Auditory Auditory area Brain Calcium Calcium Channel Blockers Cell Nucleus Cells Cognitive Development Disease Dyslexia Electrophysiology (science)Emotional Flavoproteins Future Health Hearing Image Individual Inferior Colliculus Investigation Lasers Lead Light Literature Location Measurement Measures Medial geniculate body Mus Neurons Normal Range Optics Output Pathway interactions Physiology Play Population Prefrontal Cortex Preparation Process Research Personnel Role Schizophrenia Sensory Process Series Signal Transduction Slice Structure Synapses System T-Type Calcium Channels Techniques Testing Thalamic structure Time Tinnitus Work basal forebrain base capsule cognitive function design fluorescence imaging frontal lobe gamma-Aminobutyric Acid insight meetings neurophysiology neurotransmission novel patch clamp relating to nervous system response selective attention sound synaptic depression targeted treatment tool transmission process

项目摘要

DESCRIPTION (provided by applicant): The thalamic reticular nucleus (TRN) is a capsule of GABAergic neurons that partially envelops the thalamus and has been speculated to play a role in normal cognitive functions, such as selective attention, and in pathological states, such as tinnitus and schizophrenia. Therefore, understanding the function of the auditory portion of the TRN will unlock secrets not only about normal hearing, but also provide insights about how thalamocortical systems become dysfunctional in disease. Unfortunately, the impact of the TRN on sensory processing has been particularly inscrutable because of its small size and deep location. Until now, there has not been an experimental preparation that permitted measurement of thalamic and cortical activity at the cellular level while allowing simultaneous manipulation of the TRN and afferent input to the thalamus. We have recently overcome this hurdle by developing a brain slice preparation in the mouse that contains robust connectivity between the inferior colliculus, auditory thalamus, auditory TRN and the auditory cortex (Llano et al. J Neurophysiology 2014, 111(1):197). Herein, we combine the use of this new preparation with a range of optical, electrophysiological and pharmacological tools to test a novel hypothesis: that the TRN produces paradoxical rate- dependent enhancement of the transmission of neural signals as they pass through the colliculo-thalamocortical pathway. This hypothesis is based upon recent findings that the auditory TRN receives non-reciprocal input from wide-ranging neural structures, such as the frontal cortex and amygdala, and that GABAergic input from the TRN produces short windows of hyperexcitability in thalamic neurons by de-inactivating T-type calcium currents. Successful completion of this work will open the doors to future studies that examine the impact of the frontal cortex and amygdala on auditory thalamocortical systems through the TRN, and therefore will shed light on how cognitive and emotional states influence acoustic processing.

描述（由适用提供）：丘脑网状核（TRN）是GABA能神经元的囊，部分包围了丘脑，并被推测在正常的认知功能中起作用，例如选择性注意力，例如选择性注意力，例如tinnnitus and Schizizophrenia。因此，了解TRN的听觉部分的功能不仅会解锁正常听力的秘密，而且还提供了有关丘脑皮质系统如何在疾病中功能失调的见解。不幸的是，由于其尺寸较小，位置很小，因此TRN对感官处理的影响特别不足。到目前为止，还没有进行实验制剂，可以在细胞水平上测量丘脑和皮质活性，同时允许简单地操纵 TRN和传入输入到丘脑。最近，我们通过在小鼠中开发脑切片制剂来克服这一障碍，该脑切片制剂包含下丘，听觉丘脑，听觉TRN和听觉皮层之间的牢固连通性（Llano等人，J Neurophysyology 2014，111（111（1）（1）：197）。在本文中，我们将这种新制剂的使用与一系列光学，电生理和药物工具结合使用，以检验一种新的假设：TRN在神经元通过Colliculo-Thalamoportical途径时会产生矛盾的率依赖性速率增强神经元的传播。该假设是基于最近的发现，即听觉TRN从宽范围的神经元（例如额叶皮层和杏仁核）中接收到非循环的输入，并且通过去激发T-t-tymim carcements carcements carcements carcements carcarestents carcements carcements carcements carccarcements carcements carcements carcements carcements carcements carcements carcements carccium cartents calctents carccium cartents carcements。这项工作的成功完成将为未来的研究打开大门，以研究额叶皮层和杏仁核对通过TRN的听觉丘脑皮层系统的影响，因此将阐明认知和情感状态如何影响声学处理。