NEPHROLITHIASIS PHENONOTYPING AND BIOBANK CORE

肾结石表型和生物样本库核心

• 批准号: 8907764
• 负责人: John C Lieske
• 金额: $ 14.1万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8907764
• 关键词: Affect; Bioinformatics; Biological Assay; Biopsy; Blood; Calculi; Cell Communication; Cells; Crystallization; Data; Data Set; Development; Disease; Electrolytes; Event; Explosion; Future; Growth; Individual; Instruction; Kidney; Kidney Calculi; Knowledge; Measurement; Metabolic; Mission; Nephrolithiasis; Pain; Patients; Phenotype; Pilot Projects; Population; Prevention; Process; Proteins; Proteomics; Renal Tissue; Research; Research Personnel; Resources; Risk; Sampling; Specimen; Staging; Staining method; Stains; Stratification; Techniques; Technology; Urine; Urology; biobank; calcification; economic cost; improved; inhibitor/antagonist; mass spectrometer; new therapeutic target; research study; tool; treatment strategy; urinary

PROJECT SUMMARY (See instructions): Nephrolithiasis is common, affecting up to 10% of the population. Symptomatic stone episodes produce significant pain and suffering, as well as great individual and societal economic costs. Much effort has been directed towards understanding metabolic abnormalities that can increase urinary supersaturation, which is clearly one key factor that initiates and favors stone growth. However, crystal-crystal and crystal-cell interactions also appear to be critical events during the early stages of stone formation. Therefore, increased understanding of the factors that modulate the interface of urinary proteins and crystals, and hence their subsequent interaction with other crystals or cells, is a necessary prerequisite for identifying new therapeutic targets. Such knowledge will in turn enable development of new strategies for the treatment and prevention of renal stone disease. Therefore, the urinary phenotyping core will serve 4 key functions in support of this Urology O'Brien Center: 1) Quantification of urinary components of the supersaturation profile 2) Quantification of urinary inhibitor activity 3) Renal biopsy processing, including staining of sections, and quantification of calcifications 3) Biobanking of urine, blood, renal tissues, and stones The Urinary Phenotyping Core will directly support Projects 2 and 3, and it will likely be of value for pilot projects initiated during the Center's activities that require biobanked samples or urinary assays.

项目摘要（请参阅说明）： 肾结石病很常见，影响多达10％的人口。有症状的石头发作会产生严重的疼痛和痛苦，以及巨大的个人和社会经济成本。 Much effort has been directed towards understanding metabolic abnormalities that can increase urinary supersaturation, which is clearly one key factor that initiates and favors stone growth.然而，在石材形成的早期阶段，晶体晶体和晶体细胞相互作用似乎也是关键事件。 Therefore, increased understanding of the factors that modulate the interface of urinary proteins and crystals, and hence their subsequent interaction with other crystals or cells, is a necessary prerequisite for identifying new therapeutic targets.这种知识反过来又可以制定用于治疗和预防肾脏疾病的新策略。 因此，尿液表型核心将提供4个关键功能，以支持该泌尿科中心： 1）定量过饱和度的尿成分 2）定量尿液抑制剂活性 3）肾脏活检加工，包括切片的染色和钙化的定量 3）尿液，血液，肾组织和石头的生物群 The Urinary Phenotyping Core will directly support Projects 2 and 3, and it will likely be of value for pilot projects initiated during the Center's activities that require biobanked samples or urinary assays.