COBRE: Smooth Muscle Plasticity

COBRE：平滑肌可塑性

• 批准号: 8857510
• 负责人: KENTON M SANDERS
• 金额: $ 107.62万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8857510
• 关键词: COBRE; Smooth; Muscle; Plasticity

DESCRIPTION (provided by applicant): In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases l&ll. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Principal Investigator and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.

描述（由申请人提供）：在第三阶段 COBRE 中，我们将通过继续和加强 COBRE 第 l 和 ll 阶段开发的核心实验室服务，继续开发主题重点：平滑肌可塑性。平滑肌是包含多种细胞类型的复杂组织。将这些肌肉视为同质组织一直是评估基因表达以及在发育和衰老、糖尿病、重塑、炎症反应等条件下基因表达发生变化的常用方法。内华达州率先使用细胞特异性报告基因和表面抗原对平滑肌组织的细胞成分进行分类，然后对细胞特异性转录组进行全基因组评估。特定细胞类型中高度突出的基因正在使用 Fluidigm Biomark HD 技术进行评估，以确定随着平滑肌组织在发育或疾病状态中发生改变，基因表达的逐个细胞的定量变化。转录分析还伴随着使用核糖体分析技术主动翻译哪些转录本的评估。我们还创建了特定基因上调或下调的细胞系。核心 B（FACS/流式细胞术共享资源实验室）和核心 C（单细胞分子表达）将为 COBRE 的主题重点提供服务。提供了详细的计划，以增强整个大学这些核心的用户基础，并为该州的研究界创建长期可持续的核心设施。第三阶段将通过试点资助计划进行补充，该计划旨在增加大学内其他几个项目对核心的使用。试点补助金将授予合格的研究人员，以使用核心技术获取试点数据，这样核心的利用将增强补助金提案的强度，并在新的补助金资助时增加核心的使用。第三阶段由行政核心（核心 A）领导，该核心通过首席研究员和项目协调员提供领导，通过内部指导委员会提供质量控制，并通过评估协调员和外部咨询委员会提供年度评估。