COBRE: Smooth Muscle Plasticity

COBRE：平滑肌可塑性

• 批准号: 9115199
• 负责人: KENTON M SANDERS
• 金额: $ 107.62万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9115199
• 关键词: Advisory Committees; Aging; Applications Grants; Award; Behavior; Biology; Cell Line; Cells; Centers of Research Excellence; Communities; Complex; Core Facility; Data; Development; Diabetes Mellitus; Disease; Evaluation; Flow Cytometry Shared Resource; Funding; Gene Expression; Genes; Grant; Inflammation; Laboratories; Lead; Leadership; Molecular; Muscle; Nevada; Phase; Pilot Projects; Qualifying; Quality Control; Reporter; Research; Research Personnel; Research Project Grants; Services; Smooth Muscle; Sorting - Cell Movement; Surface Antigens; Techniques; Technology; Tissues; Transcript; Translating; Universities; base; cell type; design; genome-wide; programs; response; ribosome profiling; transcriptome

In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases I&II. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Program Director and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.

在第三阶段 COBRE 中，我们将继续开发主题重点：平滑肌可塑性， 继续并加强 COBRE 第一阶段和第二阶段开发的核心实验室服务。平滑肌 是包含多种细胞类型的复杂组织。将这些肌肉视为同质组织 是评估基因表达以及基因表达发生变化的常用方法 发育和衰老、糖尿病、重塑、炎症反应等状况。 内华达大学平滑肌生物学小组开创了细胞分类技术 使用细胞特异性报告基因和表面抗原来分析平滑肌组织的成分，然后执行 细胞特异性转录组的全基因组评估。特定细胞类型中高度突出的基因是 使用 Fluidigm Biomark HD 技术进行评估，以确定逐个细胞的定量变化 平滑肌组织在发育或疾病状态下改变基因表达。转录分析还伴随着使用核糖体主动翻译哪些转录本的评估 分析技术。我们还创建了特定基因上调或下调的细胞系。 核心 B（FACS/流式细胞术共享 资源实验室）和核心 C（单细胞分子表达）。提供详细计划以加强 这些核心的用户群遍布整个大学，并为这些核心创建持久的可持续核心设施 该州的研究界。第三阶段将通过试点资助计划进行补充，该计划是 旨在增加大学内多个项目对核心的使用。试点补助金将 授予合格的研究人员使用核心技术获取试点数据，以便利用 核心将增强赠款提案的强度，并在新赠款获得资助时增加核心的使用。 第三阶段由行政核心（核心 A）领导，该核心通过项目总监提供领导 和项目协调员，通过内部指导委员会进行质量控制，并通过 评估协调员和外部咨询委员会。