Autoclave Improvements for UA Animal Facilities

UA 动物设施的高压灭菌器改进

• 批准号: 8712089
• 负责人: DAVID G BESSELSEN
• 金额: $ 47.89万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712089
• 关键词: Accounting; Address; Affect; Aging; American; Animal Experimentation; Animals; Area; Arizona; Arthritis; Award; Behavior; Biomedical Engineering; Cessation of life; Development; Diabetes Mellitus; Disease; Engineering; Equipment and supply inventories; Faculty; Frequencies; Funding; Future; Genetically Engineered Mouse; Genomics; Goals; Grant; Health; Health Sciences; Healthy People 2020; Heart Diseases; Immune; Individual; Infectious Disease Immunology; Injury; Life; Lung diseases; Maintenance; Malignant Neoplasms; Memory; Mus; Natural Resources; Pain; Personal Satisfaction; Physical environment; Population; Preventive; Principal Investigator; Quality of life; Quarantine; Research; Rodent; Services; Social Environment; Source; Staging; Sterility; Sterilization; Universities; Water; animal facility; disability; graduate student; improved; operation; premature; programs; repaired

SPECIFIC AIMS Research programs at the University of Arizona (UA) focus on the goals of the DHHS Healthy People 2020 initiative: attain high-quality, longer lives free of preventable disease, disability, injury, and premature death; eliminate disparities and improve the health of all groups; create social and physical environments that promote good health for all; and promote quality of life, healthy development, and healthy behaviors across all life stages. Specific areas of UA research excellence include infectious disease, immunology, cancer, heart disease, arthritis, diabetes, respiratory disease, memory and aging, pain alleviation, genomics, and biomedical engineering. Animal research is vital to these and other research programs that are growing at the UA. Animal usage has increased 30% over the past three years, and research funding and animal use is projected to at least double between now and 2020. Genetically engineered mice (GEM) and rodents used in research conducted at Animal Biosafety Level 2 (ABSL-2) are responsible for recent expansion, and will account for the vast majority of future expansion. Autoclaves are heavily relied upon to sterilize caging for GEM and to decontaminate ABSL-2 rodent caging. Autoclave reliability is a major programmatic concern, and increased autoclave capacity is needed to accommodate the rapidly expanding animal inventory at the UA. This project directly addresses these needs through replacement, addition, or upgrades of autoclaves in the three largest centralized animal facilities at the UA, affecting a total of 66,237 nasf space and impacting 106 PHS-funded grants awarded to 78 Principal Investigators with $46.8 million total annual funding. The project will vastly improve reliability, increase capacity, and provide redundancy of autoclaves in the facilities that maintain large rodent populations in sterile and ABSL-2 caging. The project will also significantly improve energy efficiency and preserve natural resources, particularly water. Improved animal facilities will aid in recruitment of research faculty, graduate students, and staff. Resulting research discoveries will enhance the health and well-being of all Americans. Specific Aim 1: Replace a 48 year old autoclave at the Arizona Health Sciences Center Animal Facility (AHSC) (47,000 gsf; 28,325 nasf). This is one of two large autoclaves located in the cage wash area that serves the largest UA animal facility and is at the end of its useful life. It is the primary autoclave used to sterilize rodent caging for GEM and immune deficient rodents for this facility. The repair frequency for this autoclave is high and parts have become extremely difficult to obtain. Specific Aim 2: Install a second autoclave at the Central Animal Facility (CAF) (40,254 gsf; 21,139 nasf). The only autoclave located in the CAF cage wash area is 25 years old and nearing the end of its useful life. It is used to sterilize rodent caging for GEM and to decontaminate non-approved source rodent quarantine and ABSL-2 rodent caging. The repair frequency for this autoclave is high, parts have become extremely difficult to obtain, one door is no longer functional, and prolonged downtime requires caging be transported one mile to and from the AHSC animal facility for sterilization. Installing a second autoclave provides redundancy in the near term and a replacement when the existing autoclave is no longer repairable. Specific Aim 3: Upgrade control units and door gaskets on two autoclaves in the BIO5 Animal Facility (BIO5) (23,040 gsf; 16,773 nasf). These two autoclaves serve the entire GEM barrier facility where only sterile mouse caging is used. Use of these autoclaves is frequently disrupted due to poorly engineered, proprietary control units that are not easily repaired, and door gaskets that must be removed and serviced at least once every 3 weeks. Together, these improvements will greatly enhance autoclave reliability, increase autoclave capacity needed for expansion, and provide redundancy to allow continued operations during preventive maintenance or repair of individual autoclaves at each of the three largest UA animal facilities.

具体目标 亚利桑那大学 (UA) 的研究项目重点关注 DHHS 健康人群 2020 目标 倡议：实现高质量、更长的寿命，避免可预防的疾病、残疾、伤害和过早死亡； 消除差异并改善所有群体的健康；创造社会和物质环境 促进所有人的健康；促进所有人的生活质量、健康发展和健康行为 人生阶段。 UA 研究卓越的特定领域包括传染病、免疫学、癌症、心脏 疾病、关节炎、糖尿病、呼吸系统疾病、记忆与衰老、疼痛缓解、基因组学和生物医学 工程。动物研究对于这些以及 UA 正在开展的其他研究项目至关重要。 过去三年动物使用量增加了 30%，预计研究经费和动物使用量 从现在到 2020 年，这一数字至少增加一倍。 研究中使用的基因工程小鼠 (GEM) 和啮齿类动物 在动物生物安全 2 级 (ABSL-2) 进行的测试负责近期的扩展，并将考虑到 绝大多数未来的扩张。 GEM 的笼养过程严重依赖高压灭菌器进行消毒， 对 ABSL-2 啮齿动物笼具进行净化。高压灭菌器的可靠性是一个主要的规划问题，并且增加 需要高压灭菌器容量来适应 UA 迅速扩大的动物库存。这个项目 通过更换、添加或升级三个最大的高压灭菌器来直接满足这些需求 UA 的集中动物设施，总共影响 66,237 个 NASF 空间，并影响 106 个 PHS 资助的空间 向 78 名主要研究人员提供了年度资助总额 4680 万美元的资助。该项目将极大地 提高可靠性，增加容量，并在维护大型设备的设施中提供高压灭菌器的冗余 无菌和 ABSL-2 笼养的啮齿动物种群。该项目还将显着提高能源效率 保护自然资源，特别是水。改善动物设施将有助于招募研究人员 教师、研究生和工作人员。由此产生的研究发现将增强人们的健康和福祉 所有美国人。 具体目标 1：更换亚利桑那州健康科学中心动物设施中已有 48 年历史的高压灭菌器 (AHSC)（47,000 gsf；28,325 nasf）。这是位于笼清洗区域的两个大型高压灭菌器之一 为最大的 UA 动物设施提供服务，并且已达到其使用寿命。它是用于的主要高压灭菌器 对该设施的 GEM 啮齿动物笼舍和免疫缺陷啮齿动物进行消毒。此修复频率 高压釜成本很高，零件变得极其难以获得。 具体目标 2：在中央动物设施 (CAF)（40,254 gsf；21,139 nasf）安装第二个高压灭菌器。这 位于 CAF 笼清洗区域的唯一高压灭菌器已使用 25 年，即将达到其使用寿命。这是 用于对GEM的啮齿动物笼具进行消毒，以及对未经批准的来源啮齿动物检疫和消毒进行净化 ABSL-2 啮齿动物笼养。该高压釜维修频率高，零件极难维修 获得，一扇门不再起作用，并且长时间停机需要将笼子运输一英里才能到达 并来自 AHSC 动物设施进行绝育。安装第二个高压灭菌器可提供冗余 近期内，当现有高压灭菌器无法维修时进行更换。 具体目标 3：升级 BIO5 动物设施 (BIO5) 两个高压灭菌器的控制单元和门垫片 （23,040 gsf；16,773 nasf）。这两个高压灭菌器服务于整个 GEM 屏障设施，其中只有无菌小鼠 使用笼养。由于设计不当、专有控制不当，这些高压灭菌器的使用经常受到干扰 不易维修的装置，以及必须至少每 3 次拆下和维修一次的门垫圈 几周。 总之，这些改进将大大提高高压灭菌器的可靠性，增加所需的高压灭菌器容量 用于扩展，并提供冗余以允许在预防性维护或维修期间继续运行 三个最大的 UA 动物设施中每个都有独立的高压灭菌器。