Autoclave Improvements for UA Animal Facilities

UA 动物设施的高压灭菌器改进

• 批准号: 8712089
• 负责人: DAVID G BESSELSEN
• 金额: $ 47.89万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712089
• 关键词: Accounting; Address; Affect; Aging; American; Animal Experimentation; Animals; Area; Arizona; Arthritis; Award; Behavior; Biomedical Engineering; Cessation of life; Development; Diabetes Mellitus; Disease; Engineering; Equipment and supply inventories; Faculty; Frequencies; Funding; Future; Genetically Engineered Mouse; Genomics; Goals; Grant; Health; Health Sciences; Healthy People 2020; Heart Diseases; Immune; Individual; Infectious Disease Immunology; Injury; Life; Lung diseases; Maintenance; Malignant Neoplasms; Memory; Mus; Natural Resources; Pain; Personal Satisfaction; Physical environment; Population; Preventive; Principal Investigator; Quality of life; Quarantine; Research; Rodent; Services; Social Environment; Source; Staging; Sterility; Sterilization; Universities; Water; animal facility; disability; graduate student; improved; operation; premature; programs; repaired

SPECIFIC AIMS Research programs at the University of Arizona (UA) focus on the goals of the DHHS Healthy People 2020 initiative: attain high-quality, longer lives free of preventable disease, disability, injury, and premature death; eliminate disparities and improve the health of all groups; create social and physical environments that promote good health for all; and promote quality of life, healthy development, and healthy behaviors across all life stages. Specific areas of UA research excellence include infectious disease, immunology, cancer, heart disease, arthritis, diabetes, respiratory disease, memory and aging, pain alleviation, genomics, and biomedical engineering. Animal research is vital to these and other research programs that are growing at the UA. Animal usage has increased 30% over the past three years, and research funding and animal use is projected to at least double between now and 2020. Genetically engineered mice (GEM) and rodents used in research conducted at Animal Biosafety Level 2 (ABSL-2) are responsible for recent expansion, and will account for the vast majority of future expansion. Autoclaves are heavily relied upon to sterilize caging for GEM and to decontaminate ABSL-2 rodent caging. Autoclave reliability is a major programmatic concern, and increased autoclave capacity is needed to accommodate the rapidly expanding animal inventory at the UA. This project directly addresses these needs through replacement, addition, or upgrades of autoclaves in the three largest centralized animal facilities at the UA, affecting a total of 66,237 nasf space and impacting 106 PHS-funded grants awarded to 78 Principal Investigators with $46.8 million total annual funding. The project will vastly improve reliability, increase capacity, and provide redundancy of autoclaves in the facilities that maintain large rodent populations in sterile and ABSL-2 caging. The project will also significantly improve energy efficiency and preserve natural resources, particularly water. Improved animal facilities will aid in recruitment of research faculty, graduate students, and staff. Resulting research discoveries will enhance the health and well-being of all Americans. Specific Aim 1: Replace a 48 year old autoclave at the Arizona Health Sciences Center Animal Facility (AHSC) (47,000 gsf; 28,325 nasf). This is one of two large autoclaves located in the cage wash area that serves the largest UA animal facility and is at the end of its useful life. It is the primary autoclave used to sterilize rodent caging for GEM and immune deficient rodents for this facility. The repair frequency for this autoclave is high and parts have become extremely difficult to obtain. Specific Aim 2: Install a second autoclave at the Central Animal Facility (CAF) (40,254 gsf; 21,139 nasf). The only autoclave located in the CAF cage wash area is 25 years old and nearing the end of its useful life. It is used to sterilize rodent caging for GEM and to decontaminate non-approved source rodent quarantine and ABSL-2 rodent caging. The repair frequency for this autoclave is high, parts have become extremely difficult to obtain, one door is no longer functional, and prolonged downtime requires caging be transported one mile to and from the AHSC animal facility for sterilization. Installing a second autoclave provides redundancy in the near term and a replacement when the existing autoclave is no longer repairable. Specific Aim 3: Upgrade control units and door gaskets on two autoclaves in the BIO5 Animal Facility (BIO5) (23,040 gsf; 16,773 nasf). These two autoclaves serve the entire GEM barrier facility where only sterile mouse caging is used. Use of these autoclaves is frequently disrupted due to poorly engineered, proprietary control units that are not easily repaired, and door gaskets that must be removed and serviced at least once every 3 weeks. Together, these improvements will greatly enhance autoclave reliability, increase autoclave capacity needed for expansion, and provide redundancy to allow continued operations during preventive maintenance or repair of individual autoclaves at each of the three largest UA animal facilities.

具体目标 亚利桑那大学（UA）的研究计划专注于DHHS健康人2020年的目标 倡议：获得高质量，更长的寿命没有可预防的疾病，残疾，伤害和过早死亡； 消除差异并改善所有群体的健康；创建社会和物理环境 为所有人促进身体健康；并促进生活质量，健康发展和健康行为 生活阶段。 UA研究的特定领域包括传染病，免疫学，癌症，心脏 疾病，关节炎，糖尿病，呼吸道疾病，记忆和衰老，减轻疼痛，基因组学和生物医学 工程。动物研究对于在UA增长的这些和其他研究计划至关重要。 在过去的三年中，动物使用率增加了30％，研究资金和动物使用预计 从现在到2020年至少两倍。基因工程的小鼠（宝石）和研究中使用的啮齿动物 在动物生物安全2级（ABSL-2）进行的近期扩张责任，并将考虑 未来扩张的绝大多数。高压灭绝是用来对宝石进行消毒和 去污的ABSL-2啮齿动物笼。高压灭菌的可靠性是一个主要的程序化问题，并增加了 需要高压灭菌的能力来容纳UA快速扩张的动物库存。这个项目 直接通过更大的高压灭绝，加法或升级来解决这些需求 UA的集中动物设施，总共影响了66,237个NASF空间，并影响了106个PHS资助 授予78名主要调查员的赠款，年度为4680万美元。该项目将大大 提高可靠性，提高容量并在维持大型设施中提供高压奴的冗余 无菌和ABSL-2笼中的啮齿动物种群。该项目还将显着提高能源效率 并保存自然资源，尤其是水。改善的动物设施将有助于招募研究 教职员工，研究生和员工。最终的研究发现将增强 所有美国人。 特定目标1：在亚利桑那州健康科学中心动物设施中更换48岁的高压釜 （AHSC）（47,000 GSF； 28,325 NASF）。这是位于笼子清洗区域中的两个大型高压岩之一 为UA动物设施提供最大的服务，并在其使用寿命的尽头。它是曾经的主要高压灭菌器 将啮齿动物的笼子用于该设施的宝石和免疫缺陷啮齿动物。修复频率 高压釜很高，零件变得极为困难。 具体目标2：在中央动物设施（CAF）（40,254 GSF; 21,139 NASF）安装第二个高压灭菌器。这 只有位于Caf Cage Wash区域的高压釜已有25年的历史，并且接近其使用寿命的尽头。这是 用于将啮齿动物笼子灭入宝石，并净化未批准的源啮齿动物隔离和 ABSL-2啮齿动物笼。该高压釜的维修频率很高，零件变得极为困难 获得，一扇门不再起作用，延长的停机时间需要将笼子运输到1英里处 并从AHSC动物设施进行灭菌。安装第二个高压灭菌器可在 当现有的高压釜不再可以修复时，近期和更换。 特定目的3：在Bio5动物设施中的两个高压灭菌（BIO5）上升级控制装置和门垫圈（BIO5） （23,040 GSF; 16,773 NASF）。这两个高压奴隶为整个宝石屏障设施提供服务，只有无菌鼠标 使用笼子。由于工程较差，专有控制 不容易修复的单元，并且必须卸下和维修一次的门垫 几周。 这些改进将大大提高高压釜的可靠性，增加所需的高压灭菌能力 进行扩展，并提供冗余以允许在预防性维护或维修期间继续操作 三个最大的UA动物设施中的每个高压奴隶的单个高压杆。