CORE--EXPERIMENTAL ANIMAL FACILITY

核心——实验动物设施

• 批准号: 6563871
• 负责人: DAVID G BESSELSEN
• 金额: $ 13.05万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563871
• 关键词: biomedical facility; gene targeting; genetically modified animals; laboratory mouse

APPLICANT'S DESCRIPTION: The goal of the experimental animal core is to provide relevant mouse models to study colon tumor development and to study apoptosis in colon carcinogenesis. The mouse models will be used to evaluate genetic and extracellular factors (dietary polyamines, bile acids) that affect the apoptotic pathway and to evaluate the effects of potential chemopreventive agents. Included in these models are mice that have defective APC, p53, and NOS-2 genes, genes that have been shown or are hypothesized to be involved in the oncogenesis of colon tumors in both humans and mice. These models will allow further clarification of the biochemical mechanisms involved in colon carcinogenesis and will identify additional molecular targets for chemoprevention. The specific aims of the core are: 1) to provide relevant murine models to study colon oncogenesis and the apoptotic pathway; 2) to provide treatments, carcinogens, and dietary supplements that promote tumor development in each appropriate model; 3) to administer chemopreventive agents; 4) to provide fresh tissues from the animal study protocols to each project; and 5) to perform gross and histologic analyses of intestinal tumors that develop in the murine models. The experimental animal core provides a means by which animals needed for studies in three different projects can be shared and the results of the studies are combinative. The advantages to having a experimental animal core are: less animals are needed than if each project performed the animal studies by themselves; there is uniformity and quality control in carrying out the study protocols across each project; data generated from each study is comparable given that the information is generated from the same sources with common controls; and the core allows examination of multiple rodent models of colon cancer that would otherwise be unaffordable or unavailable.

申请人的描述：实验动物核心的目标是 提供相关的小鼠模型来研究结肠肿瘤的发展并研究 结肠癌发生的凋亡。鼠标模型将用于评估 影响影响的遗传和细胞外因子（饮食多胺，胆汁酸） 凋亡途径并评估潜在化学预防的影响 代理商。这些模型中包括的小鼠APC，p53和 NOS-2基因，已显示或假设参与的基因 人和小鼠的结肠肿瘤的肿瘤发生。这些模型将 允许进一步阐明结肠涉及的生化机制 癌变，并将确定其他分子靶标的 化学预防。核心的具体目的是：1）提供相关 研究结肠肿瘤发生和凋亡途径的鼠模型； 2）到 提供促进肿瘤的治疗，致癌物和饮食补充剂 在每个适当的模型中开发； 3）管理化学预防 代理人； 4）提供从动物研究方案到每种的新鲜组织 项目; 5）对肠道肿瘤进行总体和组织学分析 在鼠模型中发展。实验动物核心提供 可以在三个不同项目中进行研究所需的动物的手段 共享和研究结果是组合性的。优势 具有实验动物核心是：比每个动物需要更少的动物 项目自己进行了动物研究；有统一性和 在每个项目中执行研究方案时的质量控制；数据 鉴于信息是 由具有共同控件的相同来源生成；核心允许 检查多种结肠癌的啮齿动物模型 负担不起或不可用。