Mouse Core

鼠标核心

• 批准号: 7150345
• 负责人: J MICHAEL SHIPLEY
• 金额: $ 14.98万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7150345
• 关键词: animal care; animal colony; asthma; biological signal transduction; biomedical facility; biotechnology; embryonic stem cell; gene targeting; genetically modified animals; immunoregulation; laboratory mouse; respiratory epithelium; tissue /cell preparation

The purpose of the Mouse Core will be to provide a centralized service-oriented facility to insure the capacity of each participating project to produce and maintain transgenic and gene-disrupted mice. The mouse core will also serve the projects of this Program by supporting the infrastructure of the BUXCO system for making invasive resistance and compliance measurements in mice, which will be utilized by most projects. The mouse core will also carry out airway dissections that will be utilized by each project. As all projects propose to utilize a combination of these services, the Mouse Core will be an important part of this Program Project Grant. The transgenic/knockout production unit is supported by dedicated space within our animal facility and is fully equipped. In addition, the Holtzman laboratory routinely utilizes the BUXCO system and carries out isolations of mouse airways, and a technician in his laboratory who is proficient in these techniques will be the individual doing these experiments for projects in this Program. This core was developed in our Pulmonary Division in 1995 and has been extensively utilized by Project Investigators, Co-Investigators, and Core Leaders on this application for many years (Holtzman, Green, Brody, Walter, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has been responsible for generating well over 100 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will further serve to facilitate collaborative interactions amongst Project Leaders. The Mouse Core is currently also supported in part by another Program Project Grant (Robert M. Senior, PI) and SCOR grant (Michael J. Holtzman, PI). The core is also utilized by other investigators at Washington University. Thus, this will allow us to realize an economy of scales and lower production costs per mouse generated.

鼠标核心的目的将是提供一个集中的面向服务的设施来确保容量 每个参与项目的生产和维持转基因和基因脱离小鼠的项目。鼠标核心 还将通过支持BUXCO系统的基础架构来为该计划的项目提供服务 小鼠的侵入性抵抗和合规性测量，大多数项目都将使用。这 鼠标核心还将进行每个项目将使用的呼吸道解剖。正如所有项目所建议的 要利用这些服务的组合，鼠标核心将是该程序项目的重要组成部分 授予。转基因/淘汰生产单元得到了我们动物设施中的专用空间 并设备齐全。此外，Holtzman实验室通常会使用Buxco系统并携带 隔离鼠标航空公司，以及精通这些技术的实验室中的技术人员 成为该计划中项目的个人进行这些实验。这个核心是在我们的 1995年的肺部划分，已被项目调查人员，共同研究人员和 该申请的核心领导者多年（Holtzman，Green，Brody，Walter，Shipley）。它由 在该领域具有集体广泛专业知识和生产力的集体记录的人员。核心有 负责产生超过100多个单独的转基因和敲除线，有多个 每个的创始人/嵌合体。曾经并将由这个核心制造的许多老鼠将进一步 有助于促进项目负责人之间的协作互动。鼠标核心当前也是 另一个计划项目Grant（Robert M. Senior，PI）和SCOR Grant（Michael J. 霍尔茨曼，pi）。华盛顿大学的其他研究人员还使用了核心。因此，这将允许 我们要实现规模的经济性和每只鼠标产生的生产成本较低。