Mouse Core

鼠标核心

• 批准号: 7150345
• 负责人: J MICHAEL SHIPLEY
• 金额: $ 14.98万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7150345
• 关键词: animal care; animal colony; asthma; biological signal transduction; biomedical facility; biotechnology; embryonic stem cell; gene targeting; genetically modified animals; immunoregulation; laboratory mouse; respiratory epithelium; tissue /cell preparation

The purpose of the Mouse Core will be to provide a centralized service-oriented facility to insure the capacity of each participating project to produce and maintain transgenic and gene-disrupted mice. The mouse core will also serve the projects of this Program by supporting the infrastructure of the BUXCO system for making invasive resistance and compliance measurements in mice, which will be utilized by most projects. The mouse core will also carry out airway dissections that will be utilized by each project. As all projects propose to utilize a combination of these services, the Mouse Core will be an important part of this Program Project Grant. The transgenic/knockout production unit is supported by dedicated space within our animal facility and is fully equipped. In addition, the Holtzman laboratory routinely utilizes the BUXCO system and carries out isolations of mouse airways, and a technician in his laboratory who is proficient in these techniques will be the individual doing these experiments for projects in this Program. This core was developed in our Pulmonary Division in 1995 and has been extensively utilized by Project Investigators, Co-Investigators, and Core Leaders on this application for many years (Holtzman, Green, Brody, Walter, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has been responsible for generating well over 100 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will further serve to facilitate collaborative interactions amongst Project Leaders. The Mouse Core is currently also supported in part by another Program Project Grant (Robert M. Senior, PI) and SCOR grant (Michael J. Holtzman, PI). The core is also utilized by other investigators at Washington University. Thus, this will allow us to realize an economy of scales and lower production costs per mouse generated.

Mouse Core 的目的是提供一个集中的面向服务的设施，以确保容量 每个参与项目的生产和维持转基因和基因破坏小鼠的费用。鼠标核心 还将通过支持 BUXCO 系统的基础设施来服务于该计划的项目，以实现 小鼠的侵入性抵抗力和顺应性测量，这将被大多数项目所利用。这 mouse core 还将进行气道解剖，供每个项目使用。正如所有项目所建议的那样 为了利用这些服务的组合，鼠标核心将成为该计划项目的重要组成部分 授予。转基因/基因敲除生产单元由我们动物设施内的专用空间提供支持 并且设施齐全。此外，Holtzman 实验室经常使用 BUXCO 系统并进行 小鼠气道的隔离，他的实验室中精通这些技术的技术人员将 是为本计划中的项目进行这些实验的个人。该核心是我们开发的 肺科于 1995 年提出，并已被项目研究者、联合研究者和 多年来该应用程序的核心领导者（Holtzman、Green、Brody、Walter、Shipley）。它的工作人员是 人员在该领域拥有广泛的专业知识和生产力记录。核心有 负责产生超过 100 个独立的转基因和敲除品系，其中有多个 每个的创始人/嵌合体。许多已经和将要由该核心制造的老鼠将进一步 旨在促进项目负责人之间的协作互动。鼠标核心目前也 部分由另一项计划项目拨款（Robert M. Senior，PI）和 SCOR 拨款（Michael J. 霍尔兹曼，PI）。华盛顿大学的其他研究人员也使用了该核心。因此，这将允许 我们能够实现规模经济并降低每只小鼠的生产成本。