Core C

核心C

• 批准号: 6823521
• 负责人: J MICHAEL SHIPLEY
• 金额: $ 11.37万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6823521
• 关键词: biomedical facility; biotechnology; disease /disorder model; gene targeting; genetically modified animals; inflammation; laboratory mouse; lung; lung development; lung injury; microinjections; regeneration; smoke inhalation; tobacco; transfection /expression vector

The purpose of the Mouse Core will be to provide a centralized service oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The Mouse Core will also serve the projects by supporting the infrastructure of a lung injury model that will be extensively utilized by most of the projects, namely exposure to cigarette smoke. As most of the projects propose to make multiple transgenic or gene-targeted lines of mice and to test these lines in the smoking model, the Mouse Core will be an important part of this Program Project Grant. The two primary components of the Mouse Core, the transgenic/knockout production unit and the smoking facility, are both supported by dedicated space within our animal facility and are fully equipped. While not previously part of this Program Project Grant, this core was developed in our Pulmonary Division in 1995 by Dr. Steven D. Shapiro, a project leader on this application, and has been extensively utilized by Project Investigators and Core Directors on this application (Senior, Shapiro, Holtzman, Parks, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has generated over 75 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will facilitate collaborative interactions amongst Project Investigators. As this Program would support 50% of the transgenic/knockout production unit, which will be utilized by Program Investigators as well as other investigators at Washington University, this will allow us to realize an economy of scales and lower production costs per mouse generated.

小鼠核心的目的是提供面向集中服务的设施，以确保每个参与项目实验室的能力生产和维持转基因和基因脱离小鼠。小鼠核心还将通过支持肺部损伤模型的基础设施来为项目提供服务，该模型将被大多数项目广泛利用，即暴露于香烟烟雾中。正如大多数项目所建议的是制造多个转基因或基因靶向小鼠的线条，并在吸烟中测试这些线条 模型，鼠标核心将是该计划项目赠款的重要组成部分。小鼠核心的两个主要组成部分，转基因/基因敲除生产单元和吸烟设施，都得到了我们动物设施中的专用空间的支持，并且配备齐全。尽管以前不是该计划项目赠款的一部分，但该核心是由该应用程序的项目负责人史蒂文·D·夏皮罗（Steven D.它由该领域的人员组成，具有集体广泛的专业知识和生产力记录。核心已经生成了75多个独立的转基因和基因敲除线，每个芯有多个创始人/嵌合体。曾经并将由该核心进行的许多小鼠将有助于项目调查人员之间的协作互动。由于该计划将支持50％的转基因/淘汰生产单元，该单元将由计划调查员以及华盛顿大学的其他研究人员使用，这将使我们能够实现规模经济和每只鼠标产生的较低生产成本。