A Biological Small Angle X-ray Scattering Station (BioSAXS) for the Compact Light

紧凑型光源生物小角X射线散射站(BioSAXS)

• 批准号: 8648498
• 负责人: RODERICK J LOEWEN
• 金额: $ 18.94万
• 依托单位: LYNCEAN TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8648498
• 关键词: Architecture; Benchmarking; Biological; Biological Process; Cells; Collimator; Complement; Complex; Crystallography; Data; Disease; Elements; Engineering; Environment; Equipment; Future; Generations; Geometry; Goals; Grant; Image; Laboratories; Laboratory Research; Light; Macromolecular Complexes; Measurement; Mechanics; Methods; Modeling; Molecular; Molecular Structure; Motion; Noise; Optics; Output; Performance; Pharmaceutical Preparations; Phase; Play; Proteins; Renaissance; Research; Resolution; Roentgen Rays; Role; Sampling; Shapes; Signal Transduction; Small Business Innovation Research Grant; Solutions; Source; Specific qualifier value; Spottings; Staging; Structure; Synchrotrons; System; Techniques; Technology; Testing; Work; X-Ray Crystallography; base; beamline; design; detector; experience; frontier; instrument; macromolecule; protein structure; prototype; public health relevance; research study; structural biology; success; tool

7. Project Summary/Abstract We propose to develop a Biological Small-angle X-ray Scattering (BioSAXS) station for the Compact Light Source, a powerful new generation of X-ray sources, which will enable biologists and biochemists to pursue state-of-the-art research in macromolecular structure studies in their own laboratories. The frontier of structural biology is the determination of large macromolecular complexes-the machines that drive the workings of the cell. X-ray crystallography is the method of choice for revealing the structures of these complexes, and has had important successes. However, since many complexes have proven to be difficult or impossible to crystallize, alternative methods are required. Lower resolution methods like SAXS can provide overall shape information of the complex that can be used, together with known substructures, for modeling its architecture. The advent of synchrotron sources together with advanced computing has led to a renaissance for SAXS as a complementary technique to crystallography for understanding a variety of molecular complexes. By developing a BioSAXS beamline and endstation, the CLS will offer biologists a new tool to perform synchrotron-like quality SAXS in their own academic or research laboratories at resolutions currently unreachable using conventional source technology. The flux of this BioSAXS beamline will exceed presently available homelab SAXS beamlines by three orders of magnitude, providing results comparable to many productive SAXS beamlines at the synchrotrons. The BioSAXS beamline is expected to complement the existing crystallography endstation already developed for the CLS to provide a unique, combined product for leading-edge biophysical analysis.

7。项目摘要/摘要 我们建议开发一个生物小角度X射线散射（Biosaxs），以进行紧凑的光 来源，有力的新一代X射线资源，它将使生物学家和生物化学家能够 在自己的实验室中从事大分子结构研究的最先进研究。边境 结构生物学的是确定大型大分子复合物 - 驱动机器的机器 细胞的工作。 X射线晶体学是揭示这些结构的首选方法 复合体，并取得了重要的成功。但是，由于许多复合物已被证明是 难以结晶或不可能结晶，需要替代方法。较低的分辨率方法等萨克斯 可以提供可以与已知子结构一起使用的复合物的总体形状信息 用于建模其体系结构。同步源的出现以及高级计算 导致萨克斯族的复兴作为晶体学的补充技术，以理解 各种分子复合物。通过开发Biosaxs光束线和端域，CL将提供 生物学家在自己的学术或研究中执行类似同步加速器的质量萨克斯的新工具 目前使用常规源技术无法实现的决议实验室。这个磁通 Biosaxs光束线将超过目前可用的Homelab saxs束线 幅度，提供的结果与同步基因的许多生产性萨克斯风要媲美。这 Biosaxs光束线有望补充现有的晶体学终端端。 CLS为领先的生物物理分析提供独特的合并产品。