Compact X-ray Station for Protein Crystallography

用于蛋白质晶体学的紧凑型 X 射线站

• 批准号: 7849375
• 负责人: RODERICK J LOEWEN
• 金额: $ 257.26万
• 依托单位: LYNCEAN TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7849375
• 关键词: Arts; Award; Charge; Communities; Computer software; Coupling; Crystallography; Data Collection; Disease; Electron Beam; Elements; Engineering; Funding; Grant; Ice; Laboratories; Lasers; Lead; Light; Mechanics; Motion; Operative Surgical Procedures; Optics; Performance; Phase; Physiologic pulse; Progress Reports; Proteins; Recovery; Research; Research Personnel; Roentgen Rays; Role; Small Business Innovation Research Grant; Source; Spottings; Structural Biologist; Structure; Synchrotrons; System; Systems Integration; Technology; Testing; Time; United States National Institutes of Health; design; improved; instrument; public health relevance; research study; response; system architecture; tool

DESCRIPTION (provided by applicant): In this Competitive Grant Revision to the Fast-Track SBIR: "A Compact X-ray Station for Protein Crystallography (CXS)," we describe proposed enhancements and upgrades to the Compact Light Source (CLS) that will result in a substantial increase in x-ray flux to the CXS. The CXS is a project to design and build a combination of x-ray optics, endstations, and software that use the monochromatic, tunable x-ray beam produced by the CLS. The CXS project, though not yet complete, has already resulted in an almost turnkey scientific instrument - a synchrotron-like x-ray system - for structural biologists at academic and corporate research centers. In order to make a significant reduction in data collection time, especially for small crystals, enhancements to the CLS are needed. In response to the NIH Notice: "NIH Announces the Availability of Recovery Act Funds for Competitive Revision Applications" - NOT-OD-09-058, we plan to expand the scope of the CXS grant to include several hardware upgrades to the CLS and to fund additional operations time. These efforts will result in significantly more x-ray flux and better reliability of the CLS, which together will greatly improve the impact and performance of the CXS. PUBLIC HEALTH RELEVANCE: The Compact X-ray Station for Protein Crystallography (CXS), along with the Compact Light Source (CLS), provides the technology to bring state-of-the-art macromolecular crystallography to structural biologists in their own local or regional laboratories. This tool will allow a wider community of researchers to conduct a broad range of structure determination experiments that will lead to better understanding of the role of proteins and other macromolecular assemblages involved in disease and therapy.

描述（由申请人提供）：在对快速轨道SBIR的这项竞争性赠款修订中：“一个紧凑型X射线晶体晶体学（CXS）”，我们描述了提议的增强功能和升级到紧凑的光源（CLS），这将导致X射线量增大，从而使CXS大幅增加。 CXS是一个设计和构建X射线光学，端站和软件的组合，该项目使用CLS生产的单色，可调X射线光束。 CXS项目虽然尚未完成，但已经为学术和企业研究中心的结构生物学家提供了一种几乎是交钥匙的科学仪器（一种类似于同步加速器的X射线系统）。为了大大减少数据收集时间，尤其是对于小晶体，需要对CLS进行增强。为了回应NIH通知：“ NIH宣布为竞争性修订申请提供恢复法案的可用性” - NOT-OD-09-058，我们计划扩大CXS赠款的范围，以在CLS中包括多个硬件升级并资助其他操作时间。这些努力将导致CLS的X射线通量和更好的可靠性，这将大大改善CXS的影响和性能。 公共卫生相关性：紧凑的蛋白质晶体学X射线电台（CXS）以及紧凑的光源（CLS）提供了一种技术，可以将最先进的大分子晶体学带给自己本地或区域实验室的结构生物学家。该工具将使更广泛的研究人员社区能够进行广泛的结构确定实验，从而更好地了解蛋白质和其他涉及疾病和治疗的大分子组合的作用。