Alternative treatments for disc degeneration: Effects on matrix homeostasis

椎间盘退变的替代治疗：对基质稳态的影响

基本信息

批准号：
8208204
负责人：
Gwendolyn A Sowa
金额：
$ 12.49万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8208204
关键词：
1 year old Acids Address Affect Age Aging-Related Process Alternative Therapies Animal Model Animals Biochemical Pathway Biological Assay Biological Availability Biological Models Biomechanics Cartilage Catabolism Cell Culture Techniques Cells Centers for Disease Control and Prevention (U.S.)Chondroitin Clinical Research Clinical Treatment Collagen Data Development Diet Dinoprostone Environment Equilibrium Extracellular Matrix Factor Analysis Future Gene Expression Glucosamine Glucosamine Sulfate Goals Height Histology Homeostasis Human In Vitro Inflammation Inflammatory Instruction Intervention Intervertebral disc structure Investigation Laboratories Lead Low Back Pain Magnetic Resonance Imaging Measurement Measures Mechanical Stimulation Mechanical Stress Mechanics Mediator of activation protein Metabolism Metalloproteases Modeling Molecular Motion Movement Nitric Oxide Nutritional Omega-3 Fatty Acids Operative Surgical Procedures Oral Oryctolagus cuniculus Outcome Pathway interactions Patients Performance Pharmaceutical Preparations Physiological Process Proteoglycan Regulatory Pathway Reporting Research Robot Role Staging Stimulus Stress Structural Genes System Techniques Testing Tissues Training Translating Translations Treatment Protocols Vertebral column Water Whole Organism Work Yoga aggrecan aggrecanase alternative treatment base chronic back pain cyclooxygenase 2 design dietary supplements experience human NOS2A protein in vivo in vivo Model indexing indium arsenide inhibitor/antagonist intervertebral disk degeneration kinematics novel nucleus pulposus prevent programs protective effect response simulation therapeutic target

项目摘要

Intervertebral disc degeneration is characterized by progressive loss of disc height related to loss of water content and proteoglycans. Key mechanisms involved in this process include inflammatory and mechanical stress. It is biologically plausible that CAM therapies, such as yoga or nutritional supplements, may exert their beneficial actions on the spine through these pathways. The fundamental goals of this research are to investigate the mechanisms behind the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on matrix balance in the intervertebral disc through use of both in vitro and in vivo systems. The immediate impact of this work is in demonstrating the biologic effects of mechanical loading and nutritional supplements on the intervertebral disc using cell culture and animal model systems. This will provide us with a powerful experimental platform with which we can test the effect of various additional loading paradigms as well as nutritional supplements on disc matrix homeostasis. Upon elucidation of the effected biochemical pathways, future studies will correlate the model systems to human spine kinematics relevant to CAM motion based therapies. This will result in the ability to translate these findings into more mechanistically directed clinical studies and rationally targeted therapeutics, which represents the long term goal of the candidate. This work and the expertise gained through the proposed training plan will serve as initial building blocks for a research program in which CAM questions can be addressed at the molecular, tissue, and whole organism level. RELEVANCE (See instructions): In a CDC report from 2004, low back pain was identified as the most common condition for which patients sought alternative treatments such as yoga and oral supplements. To utilize these interventions most effectively, it is critical to understand how these therapies are facilitating their effects. The goals of this research are to understand the effects of motion based therapies, such as yoga, and oral supplements, including glusosamine, chondroitin, and omega-3 fatty acids, on the cartilage of the spine.

椎间盘退变的特点是与水分流失相关的椎间盘高度逐渐下降含量和蛋白多糖。该过程涉及的关键机制包括炎症和机械性压力。从生物学角度来看，CAM 疗法（例如瑜伽或营养补充剂）可能会发挥作用，这在生物学上是合理的。它们通过这些途径对脊柱产生有益的作用。这项研究的基本目标是研究基于运动的疗法（例如瑜伽和口腔疗法）效果背后的机制补充剂，包括葡萄糖胺、软骨素和 omega-3 脂肪酸，可促进体内基质平衡通过使用体外和体内系统来治疗椎间盘。这项工作的直接影响是展示机械负荷和营养补充剂对椎间的生物效应使用细胞培养和动物模型系统进行光盘。这将为我们提供一个强大的实验平台我们可以用它来测试各种额外负荷范例以及营养补充剂的效果关于椎间盘基质稳态。在阐明受影响的生化途径后，未来的研究将将模型系统与基于 CAM 运动的疗法相关的人体脊柱运动学相关联。这将导致能够将这些发现转化为更具机械性指导的临床研究并合理地靶向治疗，代表候选人的长期目标。这项工作和专业知识通过拟议的培训计划获得的成果将作为研究计划的初始组成部分，其中 CAM 问题可以在分子、组织和整个生物体水平上解决。相关性（参见说明）：在 2004 年 CDC 的一份报告中，腰痛被确定为患者最常见的病症。寻求替代疗法，例如瑜伽和口服补充剂。最充分地利用这些干预措施实际上，了解这些疗法如何促进其效果至关重要。本次活动的目标研究旨在了解基于运动的疗法（例如瑜伽）和口服补充剂的效果，包括脊柱软骨上的葡萄糖胺、软骨素和 omega-3 脂肪酸。