Integrative analysis of the oral microbiome of American Indian Infants and the impact of a childhood obesity-prevention home visiting program on early childhood caries

美洲印第安婴儿口腔微生物组的综合分析以及儿童肥胖预防家访计划对儿童早期龋齿的影响

• 批准号: 10058210
• 负责人: Catherine Gayle Sutcliffe
• 金额: $ 16.57万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10058210
• 关键词: 1 year old; 16S ribosomal RNA sequencing; 2 year old; Acids; Address; Adult; Affect; Age; Age-Months; American Indians; Child; Childhood; Clinical Trials; Collaborations; Communities; Consumption; Dental Enamel; Dental caries; Development; Diet; Dietary Sugars; Dietary intake; Education; Enrollment; Environment; Epidemiologist; Family; Food; Future; Goals; Health; Health Care Costs; Health Service Area; High Prevalence; Home visitation; Infant; Infection; Intake; Intervention; Investigation; Knowledge; Metabolic; Metagenomics; Mothers; Navajo; Nutritional; Oral; Oral health; Outcome; Pain; Pathogenicity; Pattern; Performance; Prevalence; Prevention program; Probiotics; Production; Race; Randomized Controlled Trials; Research; Research Personnel; Resources; Risk; Saliva; Sampling; Schools; Speech Delay; Speech Development; Testing; Therapeutic Intervention; Time; Tribes; United States; United States Indian Health Service; aged; bacterial community; base; carbohydrate metabolism; cariogenic bacteria; demineralization; drinking water; early childhood; experience; feeding; health disparity; high risk; intervention program; liquid chromatography mass spectrometry; metabolic profile; metabolome; metabolomics; metatranscriptomics; microbial; microbial community; microbiome; microbiome composition; microbiota; microbiota profiles; next generation; obesity in children; obesity prevention; oral bacteria; oral microbial community; oral microbiome; prebiotics; programs; self esteem; sugar; sweetened beverage

PROJECT SUMMARY/ABSTRACT Early childhood caries (ECC) has devastating and long-lasting health consequences. These include higher healthcare costs, pain, infection, lower self-esteem, delayed speech development, poor performance in school, and subsequent poor oral and overall health outcomes as adults. The prevalence of ECC in children of American Indian (AI) families is considerably higher compared to all races in the United States, with ECC occurrence as early as one year of age. ECC and the closely related health disparity early childhood obesity (ECO), are critical for young AI children, especially in the Navajo community that has limited access to nutritious food and clean, safe drinking water. Diet is a crucial contributing factor to ECC and ECO, where high dietary sugar intake and early initiation of sugar-sweetened beverages (SSBs) before six months of age contribute significantly to both conditions in Navajo infants. Frequent sugar consumption drives the selection of cariogenic bacteria and associated metabolic activities within the oral microbiota that ultimately results in caries development. Acquisition and proliferation of cariogenic bacteria before two years of age have been associated with an increased risk of caries. However, the development of the oral microbiome and the impact of the sugar intake reduction on the pattern of microbiota in high-risk Navajo infants remain largely unknown. Recently, an ECO prevention program, called Family Spirit Nurture, was implemented in the Navajo Shiprock community through a randomized controlled trial. The intervention focused on infant feeding practices and SSB intake reduction. The trial enrolled mother-infant dyads and collected longitudinal oral samples from infants aged 3 to 12 months, maternal and child confounding factors, and ECC status at 1 year of age. This trial provided an unparalleled opportunity for the longitudinal investigation of Navajo infants at an early age with two important specific aims: 1) evaluate the developing oral microbiome of Navajo infants in relation to maternal and child confounding factors and the potential impact of the sugar reduction intervention on the oral microbiome profiles and caries outcomes and 2) investigate the metabolome of Navajo infants’ oral microbial communities in correlation with ECC outcomes at 12 months of age. We hypothesize that 1) the sugar reduction intervention may influence the oral microbiome composition toward non-cariogenic species and reduce ECC outcome at 12 months of age, 2) there would be developmental changes in the oral microbiome of caries-free Navajo infants and pathogenic shifts in the oral microbiome pattern for those infants who eventually develop caries, 3) Microbial communities of the caries- affected group at 12 months of age may have a higher level of carbohydrate metabolism-related metabolites compared to the caries-free group, and 4) there exists caries-related metabolite signatures of Navajo infants. The goal of this study is to evaluate if the oral microbial and metabolic profiles of Navajo infants could be used to predict ECC and to develop a therapeutic intervention through future research plans.

项目概要/摘要 儿童早期龋齿 (ECC) 会对健康造成毁灭性且长期的影响。 更高的医疗费用、疼痛、感染、自尊心降低、言语发展迟缓、表现不佳 ECC 在儿童中的患病率。 与美国所有种族相比，美洲印第安人 (AI) 家庭的比例要高得多，具有 ECC ECC 的发生早在一岁时就与健康差距密切相关。 （ECO），对于人工智能儿童来说至关重要，特别是在纳瓦霍社区，因为他们获得的机会有限 营养食品和清洁、安全的饮用水是 ECC 和 ECO 的重要影响因素。 膳食糖摄入量和六个月前尽早开始饮用含糖饮料 (SSB) 频繁食用糖会导致纳瓦霍婴儿的这两种情况发生。 口腔微生物群内的致龋细菌和相关代谢活动最终导致龋齿 两岁前致龋细菌的获得和增殖相关。 然而，口腔微生物组的发展和糖的影响会增加。 减少摄入量对高危纳瓦霍婴儿微生物群模式的影响仍然很大程度上未知。 最近，纳瓦霍族实施了一项名为“家庭精神培育”的 ECO 预防计划 Shiprock 社区通过一项随机对照试验进行干预，重点关注婴儿喂养做法。 该试验招募了母婴二人并纵向收集口腔样本。 3 至 12 个月的婴儿、母婴混杂因素以及 1 岁时的 ECC 状况。 试验为对纳瓦霍婴儿的早期纵向调查提供了无与伦比的机会 两个重要的具体目标：1）评估纳瓦霍婴儿口腔微生物群的发育与 母婴混杂因素及减糖干预对口腔的潜在影响 微生物组概况和龋齿结果，2) 研究纳瓦霍婴儿口腔微生物的代谢组 社区与 12 个月龄时 ECC 结局的相关性。 我们发现 1) 减糖干预可能会影响口腔微生物组的组成 转向非致龋物种并减少 12 月龄时的 ECC 结果，2) 无龋纳瓦霍婴儿口腔微生物组的发育变化和口腔致病性变化 最终发生龋齿的婴儿的微生物组模式，3) 龋齿的微生物群落- 12个月大时受影响的群体可能具有较高水平的碳水化合物代谢相关代谢物 与无龋组相比，4）纳瓦霍婴儿存在与龋齿相关的代谢特征。 本研究的目的是评估纳瓦霍婴儿的口腔微生物和代谢特征是否可以使用 预测 ECC 并通过未来的研究计划制定治疗干预措施。