Analytical Imaging Core

分析成像核心

基本信息

批准号：
8458592
负责人：
Hacene Boukari
金额：
$ 0.79万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8458592
关键词：
Address Biological Process Chemicals Clinical Research Commit Complex Core Facility Data Analyses Data Collection Development Diabetes Mellitus Educational process of instructing Electron Microscopy Equipment and supply inventories Evaluation Experimental Designs Freezing Goals Image Imagery Imaging Techniques Imaging technology Individual Label Laboratories Laboratory Research Metals Methodology Microscopy Mission Molecular Molecular Target Negative Staining Optics Organelles Patients Plant Resins Postdoctoral Fellow Preparation Principal Investigator Proteins Research Research Personnel Research Training Resolution Resources Sampling Scientist Services Shadowing (Histology)Students Techniques Training Work base cold temperature cost effective experience graduate student improved instrumentation light microscopy millisecond new technology nucleic acid structure pressure research study sample fixation

项目摘要

The mission of the Analytical Imaging Core Is to assist DRTC investigators in routine and complex imaging technologies for their particular research needs. This is accomplished at several levels. First, the facility has traditionally emphasized teaching new users, on an individual basis, the imaging techniques they require for successful execution of their experiments. Appropriate staff effort is expended to address the needs of these laboratories by assisting them in experimental design, data collection, quantitative analysis and presentation. Secondly, the facility offers customized full service sample preparation for electron microscopy ranging from chemical fixation, embedding in resin and ultrathin sectioning, negative staining, immunogold labeling, critical point drying and metal shadowing. In addition, the facility offers a full range of low temperature techniques for electron microscopy including quick freezing in the millisecond range by plunge, metal mirror or high pressure freezing. Thirdly, the Analytical Imaging Core develops novel technologies and state-of-the-art methodologies to continually address the ongoing needs of the Einstein DRTC research laboratories. To accomplish these goals, the Analytical Imaging Core will: 1) advise investigators, trainees and staff on the best techniques and instrumentation to address their specific research problem; 2) make available to investigators specialized light microscopy instrumentation for the analyses of cellular and molecular biological processes; 3) provide dedicated electron microscopy expertise, service and training for the high resolution visualization of cellular, organelle, protein and nucleic acid structures; 4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perform microscopy based analyses; 5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes; and 6) continue the development of cutting-edge imaging methodologies and instrumentation. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

分析成像核心的使命是协助DRTC研究人员为其特定的研究需求提供常规和复杂的成像技术。这是在多个层面上完成的。首先，该设施传统上强调了单独教授新用户，即成功执行实验所需的成像技术。花费适当的员工努力来满足这些需求实验室通过协助他们进行实验设计，数据收集，定量分析和演示。其次，该设施为电子显微镜提供了定制的全服务样品准备，从化学固定，树脂和超薄切片中的嵌入，负染色，免疫质量标签，关键点干燥和金属阴影。此外，该设施提供了全范围的低温技术用于电子显微镜，包括在毫秒范围内通过暴发，金属镜或高压冻结的快速冷冻。第三，分析成像核心开发了新型技术和最先进的方法，以不断满足爱因斯坦DRTC研究实验室的持续需求。为了实现这些目标，分析成像核心将：1）就最佳技术和仪器提供建议，以解决其特定研究问题； 2）向研究人员专门的光学显微镜仪器来分析细胞和分子生物学过程； 3）提供专用的电子显微镜专业知识，服务和培训，以高分辨率可视化细胞，细胞器，蛋白质和核酸结构； 4）提供学生，博士后研究员，调查人员和技术人员的实验室培训，以进行基于显微镜的分析所需的表演技术； 5）协助适当的数据分析评估分子靶标和生物过程； 6）继续开发前沿成像方法和仪器。所有这些服务均可提供新的糖尿病研究的调查人员，以及从事与糖尿病相关项目的研究人员，可以通过使用该核心的专业知识和设施来丰富和扩展。