Probing polymicrobial synergy using high throughput genomics

使用高通量基因组学探索多种微生物的协同作用

• 批准号: 8579262
• 负责人: Richard J Lamont
• 金额: $ 39.52万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-19 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8579262
• 关键词: Affect; American; Bacterial Infections; Biological Models; Coculture Techniques; Communities; Community Developments; Data; Development; Functional RNA; Gene Expression Profile; Gene Expression Regulation; Generations; Genes; Genetic Determinism; Genome; Genomics; Goals; Growth; Human; Human body; In Vitro; Infection; Libraries; Maps; Microbe; Modeling; Molecular; Mono-S; Natural Immunity; Operon; Oral; Oral Microbiology; Oral cavity; Organism; Pathogenesis; Periodontal Diseases; Porphyromonas gingivalis; Process; Research; Research Proposals; Resistance; Resources; Site; Study models; System; Techniques; Testing; Therapeutic; Virulence; base; fitness; in vivo; microbial; mutant; novel therapeutics; oral bacteria; oral pathogen; oral streptococci; pathogen; promoter; public health relevance; response; synergism; transcriptome sequencing

DESCRIPTION (provided by applicant): Most bacterial pathogenesis studies have focused on mono-culture infections; however, it is clear that many bacterial infections are not simply the result of colonization with a single species, but rather ensue from the action of polymicrobial communities. Microbes within polymicrobial infections often display synergistic interactions that result in enhanced colonization and persistence in the infection site. Such interactions have been particularly noted in infections of the oral cavity, although the molecular processes controlling these synergistic interactions are generally not known. Detailed mechanistic studies elucidating the polymicrobial interactions necessary for enhanced persistence in vivo is critical for a comprehensive understanding of synergy, and a necessary first step towards developing therapeutics to treat polymicrobial infections. The overall goal of this research plan is to examine, from a mechanistic standpoint, how interactions between oral bacteria impact community development and in vivo persistence. To accomplish this goal, high-throughput genomics techniques will be employed to identify microbial virulence genes that are uniquely expressed during co-infection and/or required for polymicrobial synergy.

描述（由申请人提供）：大多数细菌发病机理研究都集中在单养殖感染上；但是，很明显，许多细菌感染不仅是与单个物种定殖的结果，而且还来自多数菌群群落的作用。多生物感染中的微生物通常显示出协同的相互作用，从而增强了感染部位的定殖和持久性。尽管通常未知控制这些协同相互作用的分子过程，但这种相互作用在口腔的感染中尤其值得注意。详细的机理研究阐明了增强体内持续性所需的多肌体性相互作用对于对协同作用的全面理解至关重要，也至关重要，也是开发治疗多数菌感染的治疗剂的必要第一步。该研究计划的总体目标是从机械的角度检查口腔细菌之间的相互作用如何影响社区发展和体内持久性。为了实现这一目标，将采用高通量基因组学技术来识别在共感染期间唯一表达和/或多生物粒子协同作用的微生物毒力基因。