Mucoadhesive Polymers for Sublingual Vaccine Delivery

用于舌下疫苗递送的粘膜粘附聚合物

• 批准号: 8446264
• 负责人: LOUISE BRAUD LAWSON
• 金额: $ 22.58万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8446264
• 关键词: Adjuvant; Animals; Antibodies; Antibody Formation; Antigens; Cells; Cessation of life; Child; Communicable Diseases; Data; Development; Disease; Disease Outbreaks; Dose; Drug Formulations; Effectiveness; Feces; Film; Foundations; Health Personnel; Immune; Immune response; Immunization; Immunologics; Injection of therapeutic agent; Intestines; Investigation; Life; Liquid substance; Mass Vaccinations; Modeling; Mucosal Immunity; Mus; Needles; Polymers; Role; Safety; Serum; Surface; Syringes; Testing; Training; Vaccine Antigen; Vaccines; Virus; cost; disease transmission; in vivo; influenza virus vaccine; novel; pandemic disease; programs; protective efficacy; response; uptake; vaccine delivery

DESCRIPTION (provided by applicant): Despite great strides in development of effective vaccines, over two million deaths each year are due to vaccine-preventable diseases, indicating the need for increased accessibility to existing vaccines. In the proposed studies, we will explore a novel platform for mucosal immunization - sublingual immunization using mucoadhesive polymer films. Our hypothesis is that sublingual vaccine delivery will induce significant and protective immune responses and incorporation within mucoadhesive dissolvable polymer films containing mucosal adjuvants can enhance this response. Using inactivated influenza vaccine as a model antigen, we will perform in vivo immunization and challenge studies in a murine model to assess antibody and cellular immune responses, virus neutralization, and protective efficacy. We will determine the effectiveness of delivering influenza vaccine plus coadministered adjuvant in a single-dose mucoadhesive dissolvable film. We expect that the mucoadhesive film will result in a reduction in the amount of antigen needed to induce an immune response that is protective upon challenge. We will also better understand the role of mucosal adjuvants in modifying the immune response. These results will provide a foundation for further exploration of dissolvable films for co-administering adjuvants with inactivated influenza vaccines and other potential vaccine formulations for induction of broad mucosal protection.

描述（由申请人提供）：尽管有效疫苗的开发方面取得了长足的进步，但每年有超过200万人死亡是由于可预防疫苗的疾病引起的，这表明需要增加对现有疫苗的可及性。在拟议的研究中，我们将探索 粘膜免疫的新型平台 - 使用粘附聚合物膜使用舌下免疫。我们的假设是，舌下疫苗的递送将诱导明显的保护性免疫反应，并在含有粘膜佐剂的粘附性溶解的聚合物膜中掺入可以增强这种反应。使用被灭活的流感疫苗作为模型抗原，我们将在鼠模型中进行体内免疫和挑战研究，以评估抗体和细胞免疫反应，病毒中和和保护效率。我们将确定在单剂量粘附性溶解膜中输送流感疫苗加上共同辅助的佐剂的有效性。我们预计粘膜粘附膜将减少诱导挑战时具有保护性的免疫反应所需的抗原量。我们还将更好地了解粘膜佐剂在修饰免疫反应中的作用。这些结果将为进一步探索可溶解的薄膜提供基础，用于与毒素隔离的流感疫苗和其他潜在的疫苗配方，以诱导广泛的粘膜保护。