Liposome-Encapsulated Biodefense Vaccines

脂质体封装的生物防御疫苗

基本信息

批准号：
7228144
负责人：
LOUISE BRAUD LAWSON
金额：
$ 4.96万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7228144
关键词：
Acquired Immunodeficiency Syndrome Adult Aging Animals Anthrax disease Antibiotics Antigen Targeting Antigens Autoimmune Diseases Bioterrorism Birds Carrier Proteins Cause of Death Cells Ceramides Cessation of life Chemicals Child Communicable Diseases Dendritic Cells Deposition Drug Formulations Drug resistance Electricity Encapsulated Enhancers Epidermis Face Family suidae Health Care Costs Healthcare Immune Immune response Immunity Immunization Immunologics Immunosuppressive Agents In Vitro Inbred BALB C Mice Invasive Investigation Label Life Lipids Liposomes Lyme Disease Malaria Malignant Neoplasms Methods Modeling Molecular Weight Morbidity - disease rate Mus Needles Numbers Penetration Phase Population Preparation Proteins Relative (related person)Risk Serum Skin Societies Specialist Stratum corneum System Techniques Technology Testing Therapeutic Tissue Transplantation Topical application Training Transdermal substance administration Tuberculosis Ultrasonography Vaccine Antigen Vaccines Vesicle Virus West Nile Fever anthrax protective factor base biodefense cost epidermis cell health care delivery immune function immunogenicity influenzavirus innovation killings mortality response uptake vaccine delivery

项目摘要

DESCRIPTION (provided by applicant): This objective of this investigation is to compare different liposome formulations for the ability to deliver vaccine antigens to the dendritic cells of the epidermis and initiate an effective immune response to target antigens following transcutaneous immunization. The choice of lipids used in liposome preparation should enhance penetration of the protein antigens through the stratum corneum into the epidermis, where immunologically-responsive cells exist. The first phase of the investigation will focus on uptake through the skin using an in vitro porcine model. Delivery of fluorescently-labeled liposomes and antigenic proteins to dendritic cells in the epidermis will be visualized as well. The second phase will determine the immunologic response, both serum and mucosal, to transcutaneously applied liposome-encapsulated Protective Antigen of Bacillus anthracis in a murine model. The immunogenicity of encapsulated versus free soluble protein will be compared. Lastly, the ability of a transcutaneously applied liposomal vaccine to protect immunized mice against an anthrax challenge will be assessed. Results will provide an understanding of influence of the lipid composition of liposomes on transport of proteins administered through the skin and the immunologic response to a transcutaneous liposomal vaccine, which could offer a new technique for protection against a number of infectious diseases.

描述（由申请人提供）：这项研究的目的是比较不同脂质体配方，以便能够将疫苗抗原传递给表皮的树突状细胞，并在经皮免疫后对目标抗原产生有效的免疫反应。脂质体制备中使用的脂质的选择应增强蛋白质抗原通过角质层的渗透到存在于免疫反应性细胞的表皮中。研究的第一阶段将使用体外猪模型集中于通过皮肤吸收。也将可视化荧光标记的脂质体和抗原蛋白到表皮中的树突状细胞。第二阶段将确定血清和粘膜的免疫学反应，以在鼠模型中经皮施用脂质体封装的保护性抗原。将比较封装与游离可溶性蛋白的免疫原性。最后，将评估经皮施用的脂质体疫苗保护免疫小鼠免受炭疽挑战的能力。结果将对脂质体的脂质组成对通过皮肤施用的蛋白质的脂质组成以及对经皮脂质体疫苗的免疫反应的影响，这可以提供一种新技术，以防止多种感染性疾病。