Animal Tumor Models

动物肿瘤模型

基本信息

批准号：
8475453
负责人：
Ruby Yanru Chen-Tsai
金额：
$ 12.45万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8475453
关键词：
Animal Experimentation Animal Model Animals Antineoplastic Agents Area Brain Neoplasms Calendar Cancer Center Complex Consultations Cost Savings Data Data Analyses Data Collection Databases Development Embryo Etiology Funding Future Genes Goals Histology Histopathology Human Immunohistochemistry Individual Journals Kidney Neoplasms Knockout Mice Malignant Neoplasms Medicine Microinjections Modality Modeling Mus Mutate Neoplasm Metastasis Pathology Peer Review Pharmaceutical Preparations Procedures Process Production Prostatic Neoplasms Publications Research Research Personnel Resource Sharing Running Scientific Advances and Accomplishments Services Slide Speed Staining method Stains System Technology Testing Therapeutic Tissues Toxic effect Transgenic Mice Universities anticancer treatment cancer prevention cancer therapy comparative cost effective design embryonic stem cell in vivo medical schools meetings member mouse genome mouse model new technology oncology oncology service pancreatic neoplasm pre-clinical programs protein expression screening tissue processing tool treatment strategy tumor tumor growth

项目摘要

The Animal Tumor Models Shared Resource provides services in three major areas including transgenic and knockout mice, preclinical oncology and animal histopathology services. The transgenic and knockout mouse production services were established in 1996 at the School of Medicine with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry, and pathological interpretation. The preclinical oncology services are new additions to the Animal Tumor Models Shared Resource. These services were funded and established by the Cancer Center to support the development and testing of new compounds and to better meet the growing needs of the Cancer Center members for preclinical animal models and therapeutics. The objectives of the Shared Resource are: ¿ Consultation in all aspects of animal tumor models, including creation and analysis of tumor models and providing a database of tumor models in use by Stanford investigators ¿ Production of transgenic and knockout mice by embryo and embryonic stem cell manipulation and microinjection ¿ Full histopathology services for tissue processing, staining and pathological interpretation ¿ Preclinical oncology services for tumor induction, drug administration, toxicity studies, data collection and analysis Substantial cost savings, efficiencies and scientific advances accrue from providing these services through a centralized facility. The Shared Resource has generated over 500 transgenic and knockout mouse models in the last 5 years, each with multiple independent lines. Over 24,000 histology slides or tissue blocks were processed and over 670 pathology procedures were performed in 2008. The Shared Resource's services were acknowledged or co-authored in hundreds of publications in peer-reviewed journals. In calendar year 2008, Cancer Center members representing nine out of ten Programs utilized the Shared Resource. Future plans for the Shared Resource include implementation of tetraploid complementation to speed up knockout mouse production, expansion of the capacity of histopathology services, and offer services on complex tumor models such as orthotopic brain, prostate, pancreatic or renal tumor models.

动物肿瘤模型共享资源提供转基因等三大领域的服务和基因敲除小鼠、临床前肿瘤学和动物组织病理学服务。 1996年，医学院建立了基因敲除小鼠生产服务中心，总体规模目标是向斯坦福大学提供转基因小鼠模型和支持技术部兽医服务中心 (VSC) 的调查人员具有成本效益。比较医学负责共享资源专业的动物组织病理学服务部分。服务包括组织学、免疫组织化学和病理解释的肿瘤分析。临床前肿瘤学服务是动物肿瘤模型共享资源的新增内容。服务由癌症中心资助和建立，以支持开发和测试新化合物并更好地满足癌症中心成员对临床前日益增长的需求动物模型和治疗方法。共享资源的目标是： ¿动物肿瘤模型各方面的咨询，包括肿瘤模型的创建和分析并提供斯坦福大学研究人员使用的肿瘤模型数据库 ¿通过胚胎和胚胎干细胞操作生产转基因和基因敲除小鼠显微注射 ¿用于组织处理、染色和病理解释的全套组织病理学服务 ¿肿瘤诱导、药物管理、毒性研究、数据的临床前肿瘤学服务收集与分析通过提供这些服务可以节省大量成本、提高效率并取得科学进步共享资源已生成 500 多个转基因和基因敲除小鼠模型。在过去的 5 年里，每个都有超过 24,000 个组织学载玻片或组织块。 2008 年处理了 670 多例病理程序。共享资源的服务在同行评审期刊的数百篇出版物中得到承认或共同撰写。 2008 年，代表十分之九的癌症中心成员使用了共享资源。共享资源的未来计划包括实施四倍体互补以加速基因敲除小鼠生产，扩大组织病理学服务能力，并提供以下服务：复杂的肿瘤模型，例如原位脑、前列腺、胰腺或肾肿瘤模型。