Transgenic and Knockout Mouse Resource

转基因和基因敲除小鼠资源

• 批准号: 7826899
• 负责人: Ruby Yanru Chen-Tsai
• 金额: $ 4.44万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7826899
• 关键词: Accounting; Advanced Malignant Neoplasm; Animal Model; Biological; Cancer Center; Consult; Cost Savings; Derivation procedure; ES Cell Line; Embryo; Etiology; Foundations; Future; Gene Targeting; Genes; Goals; Human; Journals; Knockout Mice; Malignant Neoplasms; Microinjections; Molecular; Mus; Mutate; Pathogenesis; Peer Review; Plants; Production; Publications; Reagent; Research; Research Personnel; Resource Sharing; Resources; Role; Scientific Advances and Accomplishments; Services; Stem Cell Research; Stem cells; System; Technology; Transgenic Organisms; Universities; anticancer research; blastocyst; cost; embryonic stem cell; in vivo; medical schools; member; mouse genome; mouse model; programs; somatic cell nuclear transfer; tool

The Transgenic and Knockout Mouse Shared Resource was established in 1996 at the School of Medicine. The overall objective of this Shared Resource is to provide genetically modified mouse models and supporting technologies to Stanford Cancer Center investigators at cost-effective rates. This Shared Resource provides the necessary scientific and technical foundation and physical plant to conduct transgenic, gene targeting and embryonic stem cell research. The Shared Resource serves an important role in advancing cancer research by providing a wide spectrum of mouse embryo and stem cell manipulation and microinjection services to investigators as a highly specialized technology. The objectives of the Shared Resource are to: ¿ Consult in all aspects of creating and characterizing genetically modified mice ¿ Produce transgenic and knockout mice by microinjection ¿ Manipulate mouse embryonic stem cells for gene targeting ¿ Provide molecular and biological reagents associated with production of genetically modified mice Substantial cost savings, efficiencies and scientific advances accrue from providing these services through a centralized facility. The Shared Resource has generated over 500 mouse models in the last 5 years, each with multiple independent lines. The Shared Resource's services were acknowledged in more than 50 publications in peer-reviewed journals. In fiscal year 2005, there were 192 service requests, with Cancer Center members accounting for 56% of overall use. Cancer Center members representing eight out of ten Programs utilized the Shared Resource. Future plans for the Shared Resource include implementation of somatic cell nuclear transfer and tetraploid blastocyst complementation services, and derivation of new ES cell lines.

转基因和淘汰鼠标共享资源于1996年在学校建立 医学。共享资源的总体目标是提供基因修饰的鼠标 以具有成本效益的价格为斯坦福大学中央研究人员提供支持 共享资源提供了必要的科学技术基础和物理工厂 进行转基因，基因靶向和胚胎干细胞研究 通过提供广泛的小鼠胚胎和茎来推进癌症研究中的重要作用 细胞操作和对研究人员的微观注射作为高度专业化的技术 共享资源的目标是： 挥之不去在创建和表征转基因小鼠的各个方面咨询 挥之不去通过微型生产转基因和淘汰小鼠 挥之不去操纵小鼠胚胎干细胞进行基因靶向 挥之不去提供与遗传改性小鼠的产生相关的分子和生物试剂分配 提供这些服务的大量成本节省，效率和科学进步 通过集中式设施。 几年，都有多个独立线路。 2005财年的同行评审期刊中有50多个出版物，有192个服务请求， 癌症中心成员占整体使用的56％。 十分之八的计划利用了共享资源。 实施体细胞核转移和四倍体胚泡竞争服务，以及 新ES细胞系的推导。