THE GLYCOME OF SIALYLATION MUTANTS

唾液酸化突变体的糖蛋白

• 批准号: 8363037
• 负责人: MICHAEL TIEMEYER
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363037
• 关键词: Cytidine Monophosphate N-Acetylneuraminic Acid; Detection; Drosophila genus; Drosophila melanogaster; Enzymes; Funding; Grant; Methods; Minor; National Center for Research Resources; Organism; Polysaccharides; Principal Investigator; Process; Production; Research; Research Infrastructure; Resources; Sialyltransferases; Source; Structure; System; Technology; Tissues; United States National Institutes of Health; cost; mutant; sialylation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans from small amount of tissue. As a challenge to these methods, we have undertaken the detection of sialylated glycan processing intermediates in wild-type and mutant strains of Drosophila melanogaster, an organism that produces very small amounts of sialylated glycans. The Panin lab has amassed mutants in enzymes necessary for the production, transport, and utilization of CMP-sialic acid, as well as in enzymes that make glycan structures that are acceptors for the Drosophila sialyltransferase. The Tiemeyer lab has analyzed glycan profiles for mutant tissues provided by the Panin lab.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 为支持 NCRR 生物医学糖组学资助目标而开发的技术适用于许多不同的系统。 用于聚糖表征的无偏差方法足够灵敏，可以检测少量组织中的次要聚糖。 作为对这些方法的挑战，我们在黑腹果蝇的野生型和突变株中检测唾液酸化聚糖加工中间体，果蝇是一种产生极少量唾液酸化聚糖的生物体。 Panin 实验室已经积累了 CMP 唾液酸生产、运输和利用所必需的酶的突变体，以及产生作为果蝇唾液酸转移酶受体的聚糖结构的酶的突变体。 Tiemeyer 实验室分析了 Panin 实验室提供的突变组织的聚糖谱。