PROTEIN O-GLYCOSYLATION IN CRYPTOCOCCUS NEOFORMANS

新型隐球菌中的蛋白质 O-糖基化

• 批准号: 8363117
• 负责人: MICHAEL TIEMEYER
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363117
• 关键词: Cryptococcus; Cryptococcus neoformans; Detection; Funding; Genes; Grant; Methods; Minor; Morphology; National Center for Research Resources; Polysaccharides; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; System; Technology; United States National Institutes of Health; capsule; cost; glycosylation; man; mutant; novel; phosphodiester

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans. The Doering lab has previously generated Cryptococcus mutants that present altered capsule morphology as a result of altered glycan synthesis. The glycan products of these mutant genes are not always known. We have used our glycomic technology to identify a novel phosphodiester linkage between Xyl and Man as a product of a specific xylosylphosphotransferase.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 为支持 NCRR 生物医学糖组学资助目标而开发的技术适用于许多不同的系统。 用于聚糖表征的无偏差方法足够灵敏，可以检测少量聚糖。 Doering 实验室之前已经产生了隐球菌突变体，由于聚糖合成的改变，其荚膜形态发生了改变。 这些突变基因的聚糖产物并不总是已知的。 我们使用我们的糖组技术来鉴定 Xyl 和 Man 之间的新型磷酸二酯键，作为特定木糖基磷酸转移酶的产物。