THE GLYCOME OF SIALYLATION MUTANTS

唾液酸化突变体的糖蛋白

• 批准号: 8170793
• 负责人: MICHAEL TIEMEYER
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170793
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Cytidine Monophosphate N-Acetylneuraminic Acid; Detection; Drosophila genus; Drosophila melanogaster; Enzymes; Funding; Grant; Institution; Methods; Minor; National Center for Research Resources; Organism; Polysaccharides; Process; Production; Research; Research Personnel; Resources; Sialyltransferases; Source; Structure; System; Technology; Tissues; United States National Institutes of Health; mutant; sialylation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans from small amount of tissue. As a challenge to these methods, we have undertaken the detection of sialylated glycan processing intermediates in wild-type and mutant strains of Drosophila melanogaster, an organism that produces very small amounts of sialylated glycans. The Panin lab has amassed mutants in enzymes necessary for the production, transport, and utilization of CMP-sialic acid, as well as in enzymes that make glycan structures that are acceptors for the Drosophila sialyltransferase. The Tiemeyer lab has analyzed glycan profiles for mutant tissues provided by the Panin lab.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 开发的技术是为了支持NCRR生物医学甘氨酸赠款的目标，适用于许多不同的系统。 聚糖表征的非偏置方法足够敏感，以允许从少量组织中检测出较小的聚糖。 作为对这些方法的挑战，我们已经进行了检测到果蝇的野生型和突变体菌株中的硫化聚糖加工中间体，这是一种生物，生产少量的硫化聚糖。 Panin Lab在生产，运输和利用CMP-sialic酸所需的酶中积聚了突变体，以及在使果蝇siallyllansferase的受体的聚糖结构的酶中。 Tiemeyer Lab已分析了Panin Lab提供的突变组织的聚糖谱。