DEVELOPMENT OF SPECIFIC MICROSPHERE-BASED PROTEASE ASSAYS

基于特定微球的蛋白酶测定的开发

• 批准号: 8361758
• 负责人: STEVEN W GRAVES
• 金额: $ 1.12万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361758
• 关键词: Biological Assay; Collaborations; Development; Flow Cytometry; Funding; Grant; Measurement; Microspheres; National Center for Research Resources; Peptide Hydrolases; Principal Investigator; Research; Research Infrastructure; Resources; Screening procedure; Sorting - Cell Movement; Source; United States National Institutes of Health; base; cost; small molecule libraries

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This collaboration is an effort to develop high throughput protease assays for use on the Hypercyte platform at UNM. At LANL we are developing microsphere based protease assays that use fluorescent substrates attached to microspheres and analyzing them by conventional flow cytometry. At UNM we are incorporating those assays into the Hypercyte platform for high throughput measurements and chemical library screening.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 这种合作是为了开发高吞吐量蛋白酶测定法，以在UNM的Hypercyte平台上使用。 在LANL，我们正在开发基于微球的蛋白酶测定法，该测定法使用与微球相连的荧光底物并通过常规流式细胞仪对其进行分析。 在UNM，我们将这些测定纳入高培养基平台，以进行高通量测量和化学库筛选。