MICROFABRICATION FOR SORTING LARGE PARTICLES

用于分选大颗粒的微加工

• 批准号: 8361777
• 负责人: STEVEN W GRAVES
• 金额: $ 1.12万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361777
• 关键词: Caliber; Collaborations; Development; Devices; Dimensions; Flow Cytometry; Fluorescence; Funding; Grant; Microfabrication; Microfluidics; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Sorting - Cell Movement; Source; Techniques; Technology; United States National Institutes of Health; Universities; cost; operation; particle; research and development; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This collaboration with Dr. Mehmet Toner of the BioMEMS Center at Harvard University (a P41 Resource) is focused on applying microfabrication techniques to the development of a high-throughput large particle sorter in R&D Project 2. We are investigating passive particle manipulation within microfluidic channels. Using a variety of specific channel orientations, the BioMEMS Center has been able to show particle focusing, periodic particle spacing, and parallel streamlines with no external fields. We are investigating the use of this technology to focus particles in the 100-micron diameter range. We expect this approach to easily scale to this dimension and fabrication of multiple focusing channels on a single substrate should be straight- forward. As such we have obtained a few sample chips and analyzed focused particles in a simple fluorescence analysis device, with some success. The advantages of this approach include robustness, reproducible operation and no need for any external particle manipulation field.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 与哈佛大学BioMems中心的Mehmet Toner博士（P41资源）的这种合作旨在将微加工技术应用于R＆D Project 2中的高通量大粒子分道术的开发。我们正在研究微流体通道内的无源粒子操纵。 使用多种特定的通道方向，生物元中心已经能够显示粒子聚焦，周期性粒子间距和平行流线，而没有外部场。 我们正在研究该技术将颗粒聚焦在100微米直径范围内。 我们希望这种方法可以轻松地扩展到该维度和在单个基板上的多个焦点通道的制造。 因此，我们获得了一些样品芯片，并在简单的荧光分析设备中分析了聚焦颗粒，并获得了一些成功。 这种方法的优点包括鲁棒性，可再现的操作以及不需要任何外部粒子操纵场。